Ultrastructural changes in red blood cells following pulsed irradiation in vitro.

The careful choice of a combination of laser parameters such as wavelength, pulse duration, and dose has provided a means for confining laser energy to specific targets within tissue such as oxyhemoglobin within the cutaneous microvasculature. In the process of achieving such vascular selectivity, certain ultrastructural changes in red blood cell (RBC) cytoplasm have been observed, such as the generation of intracytoplasmic electron-lucent spherical structures. These structures, ranging in size from 80 to 1000A, were seen in RBCs exposed to laser doses at and above threshold, and appeared to represent a morphologically novel form of highly-specific tissue injury. This in vitro study using RBC in phosphate buffered saline (PBS) was undertaken to better understand the mechanism(s) that could have been responsible for these unique morphologic changes. We conclude that the intracytoplasmic electron-lucent spherical structures seen within RBCs were heat-fixed molds formed around vaporized water bubbles and were not produced by the release of oxygen from the oxyhemoglobin moiety during 577-nm laser irradiation.

[1]  John A. Parrish,et al.  Optical Properties of Human Skin , 1982 .

[2]  J. Parrish,et al.  Ultrastructural changes in human skin after exposure to a pulsed laser. , 1985, The Journal of investigative dermatology.

[3]  R.R. Anderson,et al.  Selective photothermolysis: precise microsurgery by selective absorption of pulsed radiation. , 1983, Science.

[4]  J. Parrish,et al.  The pulsed dye laser: its use at 577 nm wavelength. , 1987, The Journal of dermatologic surgery and oncology.

[5]  J. Parrish,et al.  Tunable pulsed dye laser for the treatment of benign cutaneous vascular ectasia. , 1987, Dermatologica.

[6]  J. Parrish,et al.  The effect of epidermal pigmentation on selective vascular effects of pulsed laser , 1984 .

[7]  R. Anderson,et al.  Histologic responses of port-wine stains treated by argon, carbon dioxide, and tunable dye lasers. A preliminary report. , 1986, Archives of dermatology.

[8]  G. Dohrmann,et al.  Dark and light epithelial cells in the choroid plexus of mammals. , 1970, Journal of ultrastructure research.

[9]  A J Welch,et al.  Spotsize effects on guinea pig skin following pulsed irradiation. , 1988, The Journal of investigative dermatology.

[10]  J. Parrish,et al.  Tunable dye laser (577 nm) treatment of port wine stains , 1986, Lasers in surgery and medicine.

[11]  J M Garden,et al.  Effect of dye laser pulse duration on selective cutaneous vascular injury. , 1986, The Journal of investigative dermatology.

[12]  G. Murphy,et al.  Pulsed dye laser (577 nm) treatment of portwine stains: ultrastructural evidence of neovascularization and mast cell degranulation in healed lesions. , 1988, The Journal of investigative dermatology.

[13]  J. Parrish,et al.  Effect of skin temperature on selective vascular injury caused by pulsed laser irradiation. , 1985, The Journal of investigative dermatology.

[14]  J. Parrish,et al.  Ultrastructure: effects of melanin pigment on target specificity using a pulsed dye laser (577 nm). , 1987, The Journal of investigative dermatology.