Antigenotoxicity protection of Carapa guianensis oil against mitomycin C and cyclophosphamide in mouse bone marrow.

The aim of this study was to evaluate the possible protective of C. guianensis oil against MMC and CP, which are direct- and indirect-acting chemical mutagens, using the micronucleus test. Three experiments were performed. First the C. guianensis oil was co-administered to mice at doses of 250, 500 and 1000 mg/kg bw with 4 mg/kg bw MMC or 50 mg/kg bw CP. Second, the mutagenic drug (CP) was administered ip 50 mg/kg bw and after 6 and 12 hours 250 and 500 mg/kg bw of C. guianensis oil were administered. In the last, C. guianensis oil was administrated (250 and 500 mg/kg bw) during five days and after it was administered ip 50 mg/kg bw CP. The results obtained showed that the C. guianensis oil is not cytotoxic neither genotoxic to mouse bone marrow. Regarding the antimutagenic effect, all doses of C. guianensis oil were significantly (p < 0.05) effective in reducing the frequency of micronucleated polychromatic erythrocytes, when compared with MMC or CP alone. Based on these results, our results suggest that the C. guianensis oil shows medicinal potential as an antimutagenic agent, modulating the mutagenicity caused by both direct- and indirect-acting chemical mutagens, in a mammalian model.

[1]  G. Jovtchev,et al.  Lilium compounds kaempferol and jatropham can modulate cytotoxic and genotoxic effects of radiomimetic zeocin in plants and human lymphocytes In vitro , 2016, Environmental toxicology.

[2]  S. Jaggi,et al.  Buccal Micronucleus Cytome Assay- A Biomarker of Genotoxicity , 2015 .

[3]  Peter F Surai.  Silymarin as a Natural Antioxidant: An Overview of the Current Evidence and Perspectives , 2015, Antioxidants.

[4]  A. Al-Asmari,et al.  A Review of Hepatoprotective Plants Used in Saudi Traditional Medicine , 2014, Evidence-based complementary and alternative medicine : eCAM.

[5]  Hesham A El-Beshbishy,et al.  Antimutagenic and anticancer activity of Al Madinah Alhasawy mint (Mentha longifolia) leaves extract. , 2014, Pakistan journal of biological sciences : PJBS.

[6]  N. Mimica-Dukić,et al.  Essential Oil of Eucalyptus Gunnii Hook. As a Novel Source of Antioxidant, Antimutagenic and Antibacterial Agents , 2014, Molecules.

[7]  Udayan Bhattacharya,et al.  Antimutagenic and anticancer activity of Darjeeling tea in multiple test systems , 2014, BMC Complementary and Alternative Medicine.

[8]  B. Dhananjaya,et al.  Antimutagenic Effect of Dioscorea Pentaphylla on Genotoxic Effect Induced By Methyl Methanesulfonate in the Drosophila Wing Spot Test , 2014, Toxicology international.

[9]  M. Eberlin,et al.  In vitro and in vivo anti-malarial activity of limonoids isolated from the residual seed biomass from Carapa guianensis (andiroba) oil production , 2014, Malaria Journal.

[10]  Jennifer A. Higgins,et al.  Anthocyans as tertiary chemopreventive agents in bladder cancer: anti-oxidant mechanisms and interaction with mitomycin C. , 2014, Mutagenesis.

[11]  A. Ahmadi,et al.  Prophylactic Efficacy of Melatonin on Cyclophosphamide-Induced Liver Toxicity in Mice , 2014, BioMed research international.

[12]  J. Dogné,et al.  Erratum to “Preventive Strategies against Bleeding due to Nonvitamin K Antagonist Oral Anticoagulants” , 2014, BioMed research international.

[13]  T. C. Machado,et al.  Assessment of the cytotoxic, genotoxic, and antigenotoxic activities of Celtis iguanaea (Jacq.) in mice. , 2013, Anais da Academia Brasileira de Ciencias.

[14]  L. Chen-Chen,et al.  Protective effects of steroidal alkaloids isolated from Solanum paniculatum L. against mitomycin cytotoxic and genotoxic actions. , 2013, Anais da Academia Brasileira de Ciencias.

[15]  M. Póvoa,et al.  Antiplasmodial activity of the andiroba (Carapa guianensis Aubl., Meliaceae) oil and its limonoid-rich fraction. , 2012, Journal of ethnopharmacology.

[16]  S. Lyu,et al.  Mutagenicity and anti-mutagenicity of Acanthopanax divaricatus var. albeofructus. , 2011, The Journal of toxicological sciences.

[17]  T. Mott,et al.  Diet of Ameerega braccata (Steindachner, 1864) (Anura: Dendrobatidae) from Chapada dos Guimarães and Cuiabá, Mato Grosso State, Brazil. , 2011, Brazilian journal of biology = Revista brasleira de biologia.

[18]  E. Palombo.  Traditional Medicinal Plant Extracts and Natural Products with Activity against Oral Bacteria: Potential Application in the Prevention and Treatment of Oral Diseases , 2011, Evidence-based complementary and alternative medicine : eCAM.

[19]  Sideney Becker Onofre,et al.  Atividade antimicrobiana do óleo-resina produzido pela copaiba - Copaifera multijuga Hayne (Leguminosae) , 2009 .

[20]  S. Hosseinimehr,et al.  Chemoprotective effects of hesperidin against genotoxicity induced by cyclophosphamide in mice bone marrow cells , 2008, Archives of pharmacal research.

[21]  G. Gajski,et al.  Efficacy of HUMN criteria for scoring the micronucleus assay in human lymphocytes exposed to a low concentration of p,p'-DDT. , 2008, Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas.

[22]  F Bakkali,et al.  Biological effects of essential oils--a review. , 2008, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[23]  M. Henriques,et al.  Antiinflammatory effects of natural tetranortriterpenoids isolated from Carapa guianensis Aublet on zymosan-induced arthritis in mice , 2006, Inflammation Research.

[24]  D. Averbeck,et al.  Antigenotoxic effects of three essential oils in diploid yeast (Saccharomyces cerevisiae) after treatments with UVC radiation, 8-MOP plus UVA and MMS. , 2006, Mutation research.

[25]  A. Collins.  Antioxidant intervention as a route to cancer prevention. , 2005, European journal of cancer.

[26]  M. Henriques,et al.  Anti-allergic effects of natural tetranortriterpenoids isolated from Carapa guianensis Aublet on allergen-induced vascular permeability and hyperalgesia , 2005, Inflammation Research.

[27]  A. Giuliani,et al.  Oxygen free radical and antioxidant defense mechanism in cancer. , 2004, Frontiers in bioscience : a journal and virtual library.

[28]  L. Antunes,et al.  Effects of the olive, extra virgin olive and canola oils on cisplatin-induced clastogenesis in Wistar rats. , 2004, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[29]  J. Lazutka,et al.  Genotoxicity of dill (Anethum graveolens L.), peppermint (Menthaxpiperita L.) and pine (Pinus sylvestris L.) essential oils in human lymphocytes and Drosophila melanogaster. , 2001, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[30]  D. Kritchevsky.  Antimutagenic and some other effects of conjugated linoleic acid , 2000, British Journal of Nutrition.

[31]  M. Fenech,et al.  The HUman MicroNucleus Project--An international collaborative study on the use of the micronucleus technique for measuring DNA damage in humans. , 1999, Mutation research.

[32]  S. De Flora,et al.  Modulation of genotoxic and related effects by carotenoids and vitamin A in experimental models: mechanistic issues. , 1999, Mutagenesis.

[33]  M. Mirotsou,et al.  Insecticidal and genotoxic activities of oregano essential oils. [Erratum: Apr 1998, v. 46 (4), p. 1694.] , 1998 .

[34]  E. Johns,et al.  Tapping an Amazônian plethora: four medicinal plants of Marajó Island, Pará (Brazil). , 1993, Journal of ethnopharmacology.

[35]  A. Bellotti,et al.  Studies on the genotoxic properties of essential oils with Bacillus subtilis rec-assay and Salmonella/microsome reversion assay. , 1991, Planta medica.

[36]  R. Kuttan,et al.  Chemopreventive activity of turmeric essential oil and possible mechanisms of action. , 2014, Asian Pacific journal of cancer prevention : APJCP.

[37]  M. Fenech,et al.  Molecular mechanisms of micronucleus, nucleoplasmic bridge and nuclear bud formation in mammalian and human cells. , 2011, Mutagenesis.

[38]  M. Henriques,et al.  Modulation of T lymphocyte and eosinophil functions in vitro by natural tetranortriterpenoids isolated from Carapa guianensis Aublet. , 2011, International immunopharmacology.

[39]  J. B. Vilar,et al.  Antimutagenicity Protection of Ginkgo Biloba Extract (egb 761) against Mitomycin C and Cyclophosphamide in Mouse Bone Marrow , 2008 .

[40]  I. Zanandrea,et al.  Atividade do óleo essêncial de orégano contra fungos patogênicos do arroz: crescimentos micelial em placas , 2004 .

[41]  P. Ringwald,et al.  In vitro antimalarial activity of limonoids from Khaya grandifoliola C.D.C. (Meliaceae). , 2000, Journal of ethnopharmacology.