The effects of embedding material, loading rate and magnitude, and penetration depth in nanoindentation of trabecular bone.

Understanding the pathophysiology of metabolic bone disease requires the characterization of both the quantity as well as the quality (i.e., microarchitecture and material properties) of the bone tissue. Nanoindentation provides a powerful yet simple method to measure the nano/micro mechanical properties of bone, but no uniform testing methodology exists. This study examines the effects of embedding materials, rate and depth of indentation, and storage time on the measured modulus. Nineteen trabecular bone samples were evaluated for the study. Although there was an 8-fold increase in the stiffness of the soft to hard epoxy, bone tissue modulus was not affected by the stiffness of the embedding materials, but hardness was affected by both the embedding material modulus, for example from 0.70 +/- 0.20 GPa (ME(low)) to 0.45 +/- 0.21 GPa (ME(Med)) (p < 0.01), and viscosity (p < 0.01). No significant differences were found with regard to the tested rates and depths of indentation for either elastic modulus or hardness. The tissue modulus tested at the 6-month time point was significantly greater in comparison with that tested at 0 or 3 months (p < 0.01). The hardness, however, did not significantly change over the span of 6 months. The results show that while nanoindentation is powerful, it is particularly sensitive to certain testing variables.

[1]  G. Pharr,et al.  Elastic properties of human cortical and trabecular lamellar bone measured by nanoindentation. , 1997, Biomaterials.

[2]  S A Goldstein,et al.  Heterogeneity of bone lamellar-level elastic moduli. , 2000, Bone.

[3]  M. Bouxsein Bone quality: an old concept revisited , 2003, Osteoporosis International.

[4]  P. Zysset,et al.  Nanoindentation discriminates the elastic properties of individual human bone lamellae under dry and physiological conditions. , 2002, Bone.

[5]  S. Goldstein,et al.  Elastic modulus and hardness of cortical and trabecular bone lamellae measured by nanoindentation in the human femur. , 1999, Journal of biomechanics.

[6]  P. Zysset,et al.  A combined atomic force microscopy and nanoindentation technique to investigate the elastic properties of bone structural units. , 2001, European cells & materials.

[7]  J Y Rho,et al.  Anisotropic properties of human tibial cortical bone as measured by nanoindentation , 2002, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[8]  Wei Lin,et al.  The influence of cortical end-plate on broadband ultrasound attenuation measurements at the human calcaneus using scanning confocal ultrasound. , 2005, The Journal of the Acoustical Society of America.

[9]  G. Pharr,et al.  The elastic properties of trabecular and cortical bone tissues are similar: results from two microscopic measurement techniques. , 1999, Journal of biomechanics.

[10]  F Peyrin,et al.  How is the indentation modulus of bone tissue related to its macroscopic elastic response? A validation study. , 2003, Journal of biomechanics.

[11]  Stefan Judex,et al.  Combining high-resolution micro-computed tomography with material composition to define the quality of bone tissue , 2003, Current osteoporosis reports.

[12]  G. Pharr,et al.  Mechanical and morphological variation of the human lumbar vertebral cortical and trabecular bone. , 1999, Journal of biomedical materials research.

[13]  G Berger,et al.  In vitro assessment of the relationship between acoustic properties and bone mass density of the calcaneus by comparison of ultrasound parametric imaging and quantitative computed tomography. , 1997, Bone.

[14]  K. J. Goodwin,et al.  Material properties of interstitial lamellae reflect local strain environments , 2002, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[15]  J. Rho,et al.  Effects of viscoelasticity and time-dependent plasticity on nanoindentation measurements of human cortical bone. , 2003, Journal of biomedical materials research. Part A.

[16]  J. Rho,et al.  Atomic force microscopy and nanoindentation characterization of human lamellar bone prepared by microtome sectioning and mechanical polishing technique. , 2003, Journal of biomedical materials research. Part A.

[17]  M. Grabois,et al.  Health Professional's Guide to Rehabilitation of the Patient with Osteoporosis , 2003, Osteoporosis International.