Biological treatment of acid mine drainage under sulphate-reducing conditions with solid waste materials as substrate

Organic waste materials were tested as the electron donors for sulphate reducers in treating acid mind drainage. They are oak chips (OC), spent oak from shiitake farms (SOS), spent mushroom compost (SMC), sludge from a wastepaper recycling plant (SWP) and organic-rich soil (ORS). The materials were placed in column reactors, which were inoculated by anaerobic digester fluid. The number of sulphate-reducers increased to 108–109 cells/ml in 2 weeks in the reactors. The reactors were continuously fed with simulated mine drainage for 35 weeks to determine sulphate reduction and metal removal. SOS, SMC and SWP served better as electron donors than OC and ORS. Metal removal efficiency of the reactor with OC was lower than those with SOS, SMC and SWP at the beginning, but became comparable at a later stage. The solvent extractable of OC inhibited the growth of sulphate-reducers. Cellulose polysaccharides were the main components of the waste materials consumed in the reactors.

[1]  A. E. Greenberg,et al.  Standard methods for the examination of water and wastewater : supplement to the sixteenth edition , 1988 .

[2]  R. C. Weast.  CRC Handbook of Chemistry and Physics , 1973 .

[3]  C. Breuil,et al.  Biological degradation of resin acids in wood chips by wood-inhabiting fungi , 1995, Applied and environmental microbiology.

[4]  H. Yamazaki,et al.  Use of Cellulosic Substrates for the Microbial Treatment of Acid Mine Drainage , 1994 .

[5]  A. Zehnder.  Biology of anaerobic microorganisms , 1988 .

[6]  J. Doré,et al.  Transformation of (sup14)C-Lignin-Labeled Cell Walls of Wheat by Syntrophococcus sucromutans, Eubacterium oxidoreducens, and Neocallimastix frontalis , 1995, Applied and environmental microbiology.

[7]  R. Bartha,et al.  The Sulphate-Reducing Bacteria , 1979 .

[8]  Robert S. Hedin,et al.  Passive Treatment of Acid Mine Drainage with Limestone , 1994 .

[9]  Byung Hong Kim,et al.  CO Fermentation of Eubacterium limosum KIST612 , 1998 .

[10]  A. Wilkins,et al.  The fate of resin acids-2. The fate of resin acids and resin acid derived neutral compounds in anaerobic sediments , 1997 .

[11]  A. Zehnder,et al.  Geochemistry and biogeochemistry of anaerobic habitats. , 1988 .

[12]  Morton A. Barlaz,et al.  Testing Anaerobic Biodegradability of Polymers in a Laboratory-Scale Simulated Landfill , 1998 .

[13]  T. Lien,et al.  Treatment of acid mine water by sulfate-reducing bacteria; results from a bench scale experiment , 1996 .

[14]  R. Hedin,et al.  Treatment of metal‐contaminated water using bacterial sulfate reduction: Results from pilot‐scale reactors , 1992, Biotechnology and bioengineering.

[15]  R. Hammack,et al.  Treatment of water from an open-pit copper mine using biogenic sulfide and limestone: A feasibility study , 1994 .

[16]  Quinn McNemar,et al.  On Growth Measurement , 1958 .

[17]  Y. Katayama,et al.  Solubilization of lignin by the ruminal anaerobic fungus Neocallimastix patriciarum , 1994, Applied and environmental microbiology.

[18]  J. Lynch,et al.  The chemical components and decomposition of wheat straw leaves, internodes and nodes , 1981 .

[19]  P. Gerhardt,et al.  Methods for general and molecular bacteriology , 1994 .

[20]  G. Gadd,et al.  Mixed sulphate-reducing bacterial cultures for bioprecipitation of toxic metals : factorial and response-surface analysis of the effects of dilution rate, sulphate and substrate concentration , 1996 .