Isolation and characterization of a 1.7-kb transposable element from a mutator line of maize.

We have cloned and sequenced a 1.7-kb Mu element from a Mutator line of maize and compared its structure to Mu1, a 1.4-kb element. With the exception of a 385-bp block of DNA present in the 1.7-kb element, these transposable elements are structurally similar, sharing terminally inverted and internal direct repeated sequences. Derivation of 1.4-kb elements from the 1.7-kb class via deletion of internal sequence is suggested by the finding that a portion of the extra DNA in Mu1.7 is part of a truncated direct repeat sequence in the 1.4-kb element. An abundant poly(A)+ RNA homologous to a portion of this extra DNA is present in several tissues of both Mutator and non-Mutator lines. Analysis of transcripts from an unstable mutant bronze 1 (bz) allele containing a Mu1.7 element inserted in an exon of the gene detects three species of poly(A)+ RNA that hybridize to a Bz1 (Bronze) gene probe: the largest contains the entire Mu1.7 element in the Bz1 gene transcript; another appears to be a spliced, chimeric transcript; the smallest is normal size Bz1 mRNA. The latter is most likely encoded by the normal-size alleles detected by Southern analysis of tissue expressing purple pigment, suggesting that normal gene function is restored by excision of the Mu1.7 element.

[1]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[2]  V. Walbot,et al.  Expression of genes transferred into monocot and dicot plant cells by electroporation. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[3]  N. Fedoroff,et al.  Deletions within a defective suppressor-mutator element in maize affect the frequency and developmental timing of its excision from the bronze locus. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[4]  J. Strommer,et al.  Insertion of an unstable element in an intervening sequence of maize Adh1 affects transcription but not processing. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[5]  V. Walbot,et al.  A deletion adjacent to the maize transposable element Mu‐1 accompanies loss of Adh1 expression. , 1985, The EMBO journal.

[6]  H. Saedler,et al.  Transposition in plants: a molecular model , 1985, The EMBO journal.

[7]  H. Saedler,et al.  Genetic and molecular analysis of the Enhancer (En) transposable element system of Zea mays , 1985, The EMBO journal.

[8]  J. Bennetzen,et al.  Nucleotide sequence of the maize transposable element Mul. , 1984, Nucleic acids research.

[9]  G. Rubin,et al.  Analysis of P transposable element functions in drosophila , 1984, Cell.

[10]  J. Bennetzen,et al.  DNA insertion in the first intron of maize Adh1 affects message levels: cloning of progenitor and mutant Adh1 alleles. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[11]  O. Nelson,et al.  Characterization of an spm-controlled bronze-mutable allele in maize. , 1984, Genetics.

[12]  G. Rubin,et al.  Structures of P transposable elements and their sites of insertion and excision in the Drosophila melanogaster genome , 1983, Cell.

[13]  J. Bennetzen,et al.  Regulatory mutants of the maize Adh1 gene caused by DNA insertions , 1982, Nature.

[14]  B. Seed.  Diazotizable arylamine cellulose papers for the coupling and hybridization of nucleic acids. , 1982, Nucleic acids research.

[15]  Stephen M. Mount,et al.  A catalogue of splice junction sequences. , 1982, Nucleic acids research.

[16]  M. Freeling,et al.  Identification of a genetic element that controls the organ-specific expression of adh1 in maize. , 1981, Genetics.

[17]  David R. Setzer,et al.  Size heterogeneity in the 3′ end of dihydrofolate reductase messenger RNAs in mouse cells , 1980, Cell.

[18]  F. Sanger,et al.  Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing. , 1980, Journal of molecular biology.

[19]  H. Dooner,et al.  Heterogeneous flavonoid glucosyltransferases in purple derivatives from a controlling element-suppressed bronze mutant in maize. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[20]  P. T. Englund.  Analysis of nucleotide sequences at 3' termini of duplex deoxyribonucleic acid with the use of the T4 deoxyribonucleic acid polymerase. , 1971, The Journal of biological chemistry.

[21]  B. Mcclintock.  Mutable Loci in Maize , 1951 .

[22]  V. Walbot,et al.  Stable transformation of maize after gene transfer by electroporation , 1986, Nature.

[23]  M. Freeling,et al.  The Mu transposable elements of maize: evidence for transposition and copy number regulation during development. , 1986, Genetics.

[24]  V. Walbot,et al.  Properties of Mutable Alleles Recovered from Mutator Stocks of Zea Mays L. , 1986 .

[25]  N. Fedoroff,et al.  Isolation of Spm controlling elements from maize. , 1984, Cold Spring Harbor symposia on quantitative biology.