Granulocyte-macrophage colony-stimulating factor, interleukin-3 (IL-3), and IL-5 greatly enhance the interaction of human eosinophils with opsonized particles by changing the affinity of complement receptor type 3.

Eosinophil functions can be modulated by several cytokines such as granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-3 (IL-3), and IL-5. We have investigated the modulatory role of these cytokines on the interaction of human eosinophils with opsonized particles (serum-treated zymosan [STZ]). Addition of STZ to eosinophils isolated from the peripheral blood of normal human donors resulted in an interaction of the STZ particles with only 15% to 25% of the cells. Treatment of the eosinophils with GM-CSF, IL-3, or IL-5 strongly enhanced both the rate of particle binding and the percentage of eosinophils binding STZ. The effect of the cytokines is most likely mediated by a change in affinity of the complement receptor type 3 (CR3) on the eosinophils for the complement fragment iC3b on the STZ particles. This is indicated by the observation that (1) the effect of the cytokines on STZ binding was prevented by a monoclonal antibody against the iC3b-binding site on CR3 and (2) the enhanced binding was already apparent before upregulation of CR3 on the cell surface was observed. In a previous study, similar results were obtained with platelet-activating factor (PAF)-primed eosinophils. Because we found that the cytokines strongly enhanced the STZ-induced PAF synthesis, we investigated the role of both released PAF and cell-associated PAF in the priming phenomenon by the cytokines. Cytokine priming appeared to be largely independent of the synthesis of PAF.

[1]  T. Andersson,et al.  Signaling properties of CR3 (CD11b/CD18) and CR1 (CD35) in relation to phagocytosis of complement-opsonized particles. , 1993, Journal of immunology.

[2]  M. Diamond,et al.  A subpopulation of Mac-1 (CD11b/CD18) molecules mediates neutrophil adhesion to ICAM-1 and fibrinogen , 1993, The Journal of cell biology.

[3]  D. Roos,et al.  Priming of human eosinophils by platelet-activating factor enhances the number of cells able to bind and respond to opsonized particles. , 1992, Journal of immunology.

[4]  L. Olgiati,et al.  Migration of primed human eosinophils across cytokine-activated endothelial cell monolayers. , 1992, Blood.

[5]  L. Koenderman,et al.  Release of platelet-activating factor is important for the respiratory burst induced in human eosinophils by opsonized particles. , 1992, Blood.

[6]  C. Page.  Mechanisms of hyperresponsiveness: platelet-activating factor. , 1992, The American review of respiratory disease.

[7]  P. Weller,et al.  Cytokine regulation of eosinophil function. , 1992, Clinical immunology and immunopathology.

[8]  M. Vadas,et al.  GM-CSF, IL-3 and IL-5: cross-competition on human haemopoietic cells. , 1992, Immunology today.

[9]  S. Bischoff,et al.  IL-3 and IL-5 prime normal human eosinophils to produce leukotriene C4 in response to soluble agonists. , 1991, Journal of immunology.

[10]  J. Lowe,et al.  Molecular cloning of a human fucosyltransferase gene that determines expression of the Lewis x and VIM-2 epitopes but not ELAM-1-dependent cell adhesion. , 1991, The Journal of biological chemistry.

[11]  T. Kuijpers,et al.  Membrane surface antigen expression on neutrophils: a reappraisal of the use of surface markers for neutrophil activation. , 1991, Blood.

[12]  T. Kuijpers,et al.  Characteristics of CR3-mediated aggregation in human eosinophils: effect of priming by platelet-activating factor. , 1991, The Journal of allergy and clinical immunology.

[13]  Paul,et al.  Modulation and induction of eosinophil chemotaxis by granulocyte-macrophage colony-stimulating factor and interleukin-3. , 1991, Blood.

[14]  L. Koenderman,et al.  Priming of the respiratory burst in human eosinophils is accompanied by changes in signal transduction. , 1990, Journal of immunology.

[15]  A. Wardlaw,et al.  IgG-dependent generation of platelet-activating factor by normal and low density human eosinophils. , 1990, Journal of immunology.

[16]  A. Wardlaw,et al.  IL-5 enhances the in vitro adhesion of human eosinophils, but not neutrophils, in a leucocyte integrin (CD11/18)-dependent manner. , 1990, Immunology.

[17]  L. Koenderman,et al.  Continuous cell activation is necessary for stable interaction of complement receptor type 3 with its counter‐structure in the aggregation response of human neutrophils , 1990, European journal of immunology.

[18]  C. Hack,et al.  Complement fragments C3b and iC3b coupled to latex induce a respiratory burst in human neutrophils. , 1990, Molecular immunology.

[19]  H. Kita,et al.  Regulatory effect of cytokines on eosinophil degranulation. , 1990, Journal of immunology.

[20]  S. Wright,et al.  Two leukocyte receptors (CD11a/CD18 and CD11b/CD18) mediate transient adhesion to endothelium by binding to different ligands. , 1989, Journal of immunology.

[21]  J. Pujol,et al.  Identification of an alveolar macrophage-derived activity in bronchial asthma that enhances leukotriene C4 generation by human eosinophils stimulated by ionophore A23187 as a granulocyte-macrophage colony-stimulating factor. , 1989, The American review of respiratory disease.

[22]  W. Owen,et al.  IL-5-dependent conversion of normodense human eosinophils to the hypodense phenotype uses 3T3 fibroblasts for enhanced viability, accelerated hypodensity, and sustained antibody-dependent cytotoxicity. , 1989, Journal of immunology.

[23]  C. Sanderson,et al.  Human interleukin-5 (IL-5) regulates the production of eosinophils in human bone marrow cultures: comparison and interaction with IL-1, IL-3, IL-6, and GMCSF. , 1989, Blood.

[24]  O. Götze,et al.  Clustering of ligand on the surface of a particle enhances adhesion to receptor-bearing cells. , 1988, The Journal of biological chemistry.

[25]  L. Koenderman,et al.  An Improved Method for the Isolation of Eosinophilic Granulocytes From Peripheral Blood of Normal Individuals , 1988, Journal of leukocyte biology.

[26]  W. Owen,et al.  Human eosinophils have prolonged survival, enhanced functional properties, and become hypodense when exposed to human interleukin 3. , 1988, The Journal of clinical investigation.

[27]  T. Suda,et al.  Highly purified murine interleukin 5 (IL-5) stimulates eosinophil function and prolongs in vitro survival. IL-5 as an eosinophil chemotactic factor , 1988, The Journal of experimental medicine.

[28]  J. Harlan,et al.  Increased surface expression of CD11b/CD18 (Mac-1) is not required for stimulated neutrophil adherence to cultured endothelium. , 1988, The Journal of clinical investigation.

[29]  J. Gamble,et al.  Recombinant human interleukin 5 is a selective activator of human eosinophil function , 1988, The Journal of experimental medicine.

[30]  S. Wright,et al.  Aggregation of complement receptors on human neutrophils in the absence of ligand , 1987, The Journal of cell biology.

[31]  W. Owen,et al.  Regulation of human eosinophil viability, density, and function by granulocyte/macrophage colony-stimulating factor in the presence of 3T3 fibroblasts , 1987, The Journal of experimental medicine.

[32]  J. David,et al.  The regulation of human eosinophil function by cytokines. , 1987, Immunology today.

[33]  S. Wright,et al.  Phorbol esters cause sequential activation and deactivation of complement receptors on polymorphonuclear leukocytes. , 1986, Journal of immunology.

[34]  C. Anderson,et al.  Human platelet Fc receptor for immunoglobulin G. Identification as a 40,000-molecular-weight membrane protein shared by monocytes. , 1985, The Journal of clinical investigation.

[35]  P. Lachmann,et al.  Membrane complement receptor type three (CR3) has lectin-like properties analogous to bovine conglutinin as functions as a receptor for zymosan and rabbit erythrocytes as well as a receptor for iC3b. , 1985, Journal of immunology.

[36]  M. Kazatchkine,et al.  Mouse monoclonal antibodies to the human C3b receptor. , 1985, Molecular immunology.

[37]  C. Figdor,et al.  Biochemical and functional characteristics of the human leukocyte membrane antigen family LFA‐1, Mo‐1 and p!50,95 , 1985, European journal of immunology.

[38]  D. Lenihan,et al.  Increased biosynthesis of platelet-activating factor in activated human eosinophils. , 1984, The Journal of biological chemistry.