Enhanced lipase recovery through RSM integrated differential evolutionary approach from the fermented biomass

The aim of this work was to apply a modeling integrated optimisation approach for a complex, highly nonlinear system for an extracellular lipase extraction process. The model was developed using mutation, crossover and selection variables of Differential Evolution (DE) based on central composite design of Response Surface Methodology. The experimentally validated model was optimized by DE, a robust evolutionary optimization tool. A maximum lipase activity of 134.13 U/gds (more than 36.28 U/gds compared to one variable at a time approach) was observed with the DE-stated optimum values of 25.01% dimethyl sulfoxide concentration, 40 mM buffer, 128.52 min soaking time and 35oC with the DE control parameters, namely number of population, generations, crossover operator and scaling factor as 20, 50, 0.5 and 0.25, respectively. The use of DE approach improved the optimization capability and decision speed, resulting in an improved yield of 36.28 U/gds compared to the one variable at a time approach for the extracellular lipase activity under the non-optimized conditions. The developed mathematical model and optimization were generic in nature, which seemed to be useful for the scale-up studies of maximum recovery of lipase from the fermented biomass.

[1]  S. Adachi,et al.  Synthesis of esters by immobilized-lipase-catalyzed condensation reaction of sugars and fatty acids in water-miscible organic solvent. , 2005, Journal of bioscience and bioengineering.

[2]  T. S. Porto,et al.  Reversed micellar extraction of an extracellular protease from Nocardiopsis sp. fermentation broth , 2005 .

[3]  R. Banerjee,et al.  Optimization of extraction parameters for recovery of alpha-amylase from the fermented bran of Bacillus circulans GRS313 , 2001 .

[4]  Sung Ok Han,et al.  Biodiesel production by a mixture of Candida rugosa and Rhizopus oryzae lipases using a supercritical carbon dioxide process. , 2011, Bioresource technology.

[5]  Vijay Kumar Garlapati,et al.  Lipase mediated isoamyl acetate synthesis in solvent-free system using vinyl acetate as acyl donor. , 2009 .

[6]  Vijay Kumar Garlapati,et al.  Enzymatic synthesis of fruit flavor esters by immobilized lipase from Rhizopus oligosporus optimized with response surface methodology , 2009 .

[7]  C. F. Prutton,et al.  Principles of Physical Chemistry , 1965 .

[8]  Y. Gargouri,et al.  Production of butyl acetate ester by lipase from novel strain of Rhizopus oryzae. , 2007, Journal of bioscience and bioengineering.

[9]  Yan Xu,et al.  Novel minor lipase from Rhizopus chinensis during solid-state fermentation: biochemical characterization and its esterification potential for ester synthesis. , 2009, Bioresource technology.

[10]  Akihiko Kondo,et al.  Lipase localization in Rhizopus oryzae cells immobilized within biomass support particles for use as whole-cell biocatalysts in biodiesel-fuel production. , 2006, Journal of bioscience and bioengineering.

[11]  Karl-Erich Jaeger,et al.  Lipases for biotechnology. , 2002, Current opinion in biotechnology.

[12]  Dharmendra S Dheeman,et al.  Purification and properties of Amycolatopsis mediterranei DSM 43304 lipase and its potential in flavour ester synthesis. , 2011, Bioresource technology.

[13]  B. Chiang,et al.  Optimization of reversed micellar extraction of chitosanases produced by Bacillus cereus , 2006 .

[14]  R. Verger,et al.  N‐terminal peptide of Rhizopus oryzae lipase is important for its catalytic properties , 2005, FEBS letters.

[15]  Ramesh Chander Kuhad,et al.  Production and recovery of an alkaline exo-polygalacturonase from Bacillus subtilis RCK under solid-state fermentation using statistical approach. , 2008, Bioresource technology.

[16]  S. Renganathan,et al.  Optimization of L-asparaginase production by Aspergillus terreus MTCC 1782 using response surface methodology and artificial neural network-linked genetic algorithm , 2012 .

[17]  S. Boufi,et al.  hysical immobilization of Rhizopus oryzae lipase onto cellulose substrate : ctivity and stability studies aha , 2008 .

[18]  Benjamin S. Duran,et al.  Statistical Methods for Engineers and Scientists , 1985 .

[19]  O. Barbosa,et al.  Effect of the immobilization protocol on the properties of lipase B from Candida antarctica in organic media: Enantiospecifc production of atenolol acetate , 2011 .

[20]  Rintu Banerjee,et al.  Enzymatic transesterification of Jatropha oil , 2009, Biotechnology for biofuels.

[21]  Yan Xu,et al.  Rhizopus chinensis lipase: Gene cloning, expression in Pichia pastoris and properties , 2009 .

[22]  A. P. Júnior,et al.  Optimization of β-xylosidase recovery by reversed micelles using response surface methodology , 2003 .

[23]  A. Kademi,et al.  Lipases and their industrial applications , 2004, Applied biochemistry and biotechnology.

[24]  V. Thangavelu,et al.  Statistical experimental design for evaluation of medium components for lipase production by Rhizopus arrhizus MTCC 2233 , 2009 .

[25]  Comeau,et al.  Purification and characterization of an extracellular lipase from a thermophilic Rhizopus oryzae strain isolated from palm fruit. , 2000, Enzyme and microbial technology.

[26]  Robert V. Brill,et al.  Applied Statistics and Probability for Engineers , 2004, Technometrics.

[27]  M. Reetz.  Lipases as practical biocatalysts. , 2002, Current opinion in chemical biology.

[28]  M. Ayub,et al.  Extraction optimization of xylanases obtained by solid-state cultivation of Bacillus circulans BL53 , 2005 .

[29]  T. Rocha-Santos,et al.  The effectiveness of a biological treatment with Rhizopus oryzae and of a photo-Fenton oxidation in the mitigation of toxicity of a bleached kraft pulp mill effluent. , 2009, Water research.

[30]  R. Banerjee,et al.  Some studies on optimization of extraction process for protease production in SSF , 1999 .

[31]  Abdul Hameed,et al.  Industrial applications of microbial lipases , 2006 .

[32]  H. Fukuda,et al.  Purification and characterization of lipase from Rhizopus chinensis cells. , 1999, Journal of bioscience and bioengineering.

[33]  Margaret J. Robertson,et al.  Design and Analysis of Experiments , 2006, Handbook of statistics.

[34]  R. Banerjee,et al.  Kinetics of solvent-free geranyl acetate synthesis by Rhizopus oligosporus NRRL 5905 lipase immobilized on to cross-linked silica , 2009 .

[35]  Rainer Storn,et al.  Differential Evolution – A Simple and Efficient Heuristic for global Optimization over Continuous Spaces , 1997, J. Glob. Optim..

[36]  R. Rodrigues,et al.  Optimization of transglutaminase extraction produced by Bacillus circulans BL32 on solid-state cultivation , 2008 .

[37]  R. M. Bethea,et al.  Statistical Methods for Engineers and Scientists. , 1985 .

[38]  Samia A. Ahmed.  Optimization of Production and Extraction Parameters of Bacillus megaterium Levansucrase Using Solid-state Fermentation , 2008 .

[39]  Rintu Banerjee,et al.  Comparative study of thermostabilty and ester synthesis ability of free and immobilized lipases on cross linked silica gel , 2008, Bioprocess and biosystems engineering.

[40]  L. Castilho,et al.  Recovery of pectolytic enzymes produced by solid state culture of Aspergillus niger , 1999 .

[41]  Y. Zhuang,et al.  Efficient extraction of intracellular reduced glutathione from fermentation broth of Saccharomyces cerevisiae by ethanol. , 2009, Bioresource technology.

[42]  R. Storn,et al.  Differential Evolution: A Practical Approach to Global Optimization (Natural Computing Series) , 2005 .

[43]  Douglas C. Montgomery,et al.  Response Surface Methodology: Process and Product Optimization Using Designed Experiments , 1995 .