An ultrasensitive electrochemical "turn-on" label-free biosensor for Hg2+with AuNP-functionalized reporter DNA as a signal amplifier.

A highly selective electrochemical biosensor for the ultrasensitive detection of Hg2+ in aqueous solution has been developed based on the strong and specific binding of Hg2+ by two DNA thymine bases (T-Hg2+-T) and the use of AuNP-functionalized reporter DNA to achieve signal amplification.

[1]  Anant Kumar Singh,et al.  Selective detection of mercury (II) ion using nonlinear optical properties of gold nanoparticles. , 2008, Journal of the American Chemical Society.

[2]  Shu Wang,et al.  A Reversible and Highly Selective Fluorescent Sensor for Mercury(II) Using Poly(thiophene)s that Contain Thymine Moieties , 2006 .

[3]  G. Shen,et al.  Aptamer-based rolling circle amplification: a platform for electrochemical detection of protein. , 2007, Analytical chemistry.

[4]  Kevin W Plaxco,et al.  Rapid, sequence-specific detection of unpurified PCR amplicons via a reusable, electrochemical sensor. , 2006, Proceedings of the National Academy of Sciences of the United States of America.

[5]  Xiaogang Liu,et al.  One-step, room temperature, colorimetric detection of mercury (Hg2+) using DNA/nanoparticle conjugates. , 2008, Journal of the American Chemical Society.

[6]  Remo Rohs,et al.  Methylene Blue Binding to DNA with Alternating GC Base Sequence: A Modeling Study , 2000 .

[7]  Chad A Mirkin,et al.  Nonenzymatic detection of bacterial genomic DNA using the bio bar code assay. , 2007, Analytical chemistry.

[8]  Chad A Mirkin,et al.  Colorimetric detection of mercuric ion (Hg2+) in aqueous media using DNA-functionalized gold nanoparticles. , 2007, Angewandte Chemie.

[9]  Chuan He,et al.  A general strategy to convert the MerR family proteins into highly sensitive and selective fluorescent biosensors for metal ions. , 2004, Journal of the American Chemical Society.

[10]  A. Heeger,et al.  Label-free electrochemical detection of DNA in blood serum via target-induced resolution of an electrode-bound DNA pseudoknot. , 2007, Journal of the American Chemical Society.

[11]  Bang-Ce Ye,et al.  Highly sensitive detection of mercury(II) ions by fluorescence polarization enhanced by gold nanoparticles. , 2008, Angewandte Chemie.

[12]  Guo-Li Shen,et al.  Reusable electrochemical sensing platform for highly sensitive detection of small molecules based on structure-switching signaling aptamers. , 2007, Analytical chemistry.

[13]  Chad A Mirkin,et al.  Chip-based scanometric detection of mercuric ion using DNA-functionalized gold nanoparticles. , 2008, Analytical chemistry.

[14]  Yi Lu,et al.  Highly sensitive "turn-on" fluorescent sensor for Hg2+ in aqueous solution based on structure-switching DNA. , 2008, Chemical communications.

[15]  Bin Liu,et al.  Highly sensitive and selective detection of Hg(2+) in aqueous solution with mercury-specific DNA and Sybr Green I. , 2008, Chemical communications.

[16]  Jian-hui Jiang,et al.  Highly specific and sensitive electrochemical genotyping via gap ligation reaction and surface hybridization detection. , 2009, Journal of the American Chemical Society.

[17]  Chuan He,et al.  Design of an emission ratiometric biosensor from MerR family proteins: a sensitive and selective sensor for Hg2+. , 2007, Journal of the American Chemical Society.

[18]  Zusing Yang,et al.  Synthesis of highly fluorescent gold nanoparticles for sensing mercury(II). , 2007, Angewandte Chemie.

[19]  Elizabeth M. Nolan,et al.  Tools and tactics for the optical detection of mercuric ion. , 2008, Chemical reviews.

[20]  Ronghua Yang,et al.  Gold nanoparticle-based colorimetric and "turn-on" fluorescent probe for mercury(II) ions in aqueous solution. , 2008, Analytical chemistry.

[21]  Ciara K O'Sullivan,et al.  Reagentless, reusable, ultrasensitive electrochemical molecular beacon aptasensor. , 2006, Journal of the American Chemical Society.

[22]  Chunhai Fan,et al.  A target-responsive electrochemical aptamer switch (TREAS) for reagentless detection of nanomolar ATP. , 2007, Journal of the American Chemical Society.

[23]  B. Nordén,et al.  Sequence-Specific Interactions of Methylene Blue with Polynucleotides and DNA: A Spectroscopic Study , 1994 .

[24]  E. Ferapontova,et al.  An RNA aptamer-based electrochemical biosensor for detection of theophylline in serum. , 2008, Journal of the American Chemical Society.

[25]  Guo-Li Shen,et al.  An optical fiber chemical sensor for mercury ions based on a porphyrin dimer. , 2002, Analytical chemistry.

[26]  Guo-Li Shen,et al.  Electrochemical aptasensor based on proximity-dependent surface hybridization assay for single-step, reusable, sensitive protein detection. , 2007, Journal of the American Chemical Society.

[27]  C. Mirkin,et al.  Nanoparticle-Based Bio-Bar Codes for the Ultrasensitive Detection of Proteins , 2003, Science.

[28]  Takashi Fujimoto,et al.  MercuryII-mediated formation of thymine-HgII-thymine base pairs in DNA duplexes. , 2006, Journal of the American Chemical Society.

[29]  Chih-Ching Huang,et al.  Oligonucleotide-based fluorescence probe for sensitive and selective detection of mercury(II) in aqueous solution. , 2008, Analytical chemistry.

[30]  Chad A Mirkin,et al.  Nanostructures in biodiagnostics. , 2005, Chemical reviews.

[31]  A. Heeger,et al.  Label-free electronic detection of thrombin in blood serum by using an aptamer-based sensor. , 2005, Angewandte Chemie.

[32]  Chunhai Fan,et al.  Optical Detection of Mercury(II) in Aqueous Solutions by Using Conjugated Polymers and Label‐Free Oligonucleotides , 2007 .

[33]  Yi Lu,et al.  Rational design of "turn-on" allosteric DNAzyme catalytic beacons for aqueous mercury ions with ultrahigh sensitivity and selectivity. , 2007, Angewandte Chemie.

[34]  Chojiro Kojima,et al.  15N-15N J-coupling across Hg(II): direct observation of Hg(II)-mediated T-T base pairs in a DNA duplex. , 2007, Journal of the American Chemical Society.

[35]  Zhiwei Zhu,et al.  Electrochemical DNAzyme sensor for lead based on amplification of DNA-Au bio-bar codes. , 2008, Analytical chemistry.

[36]  Chih-Ching Huang,et al.  Detection of mercury(II) based on Hg2+ -DNA complexes inducing the aggregation of gold nanoparticles. , 2008, Chemical communications.

[37]  Itamar Willner,et al.  Optical analysis of Hg2+ ions by oligonucleotide-gold-nanoparticle hybrids and DNA-based machines. , 2008, Angewandte Chemie.

[38]  A. Ono,et al.  Highly selective oligonucleotide-based sensor for mercury(II) in aqueous solutions. , 2004, Angewandte Chemie.