miR-142-5p Inhibits Cell Invasion and Migration by Targeting DNMT1 in Breast Cancer

Abnormal cell proliferation caused by abnormal transcription regulation mechanism seems to be one of the reasons for the progression of breast cancer and also the pathological basis. MicroRNA-142-5p (miR-142-5p) is a low-expressed miRNA in breast cancer. The role of MKL-1’s regulation of DNMT1 in breast cancer cell proliferation and migration is still unclear. MKL-1 (myocardin related transcription factor A) can bind to the conserved cis-regulatory element CC (A/T) 6GG (called CarG box) in the promoter to regulate the transcription of miR-142-5p. The expressions of miR-142-5p and MKL-1 are positively correlated. In addition, it has been proved that DNMT1 is the target of miR-142-5p, which inhibits the expression of DNMT1 by targeting the 3′-UTR of DNMT1, thereby forming a feedback loop and inhibiting the migration and proliferation of breast cancer. Our data provide important and novel insights into the MKL-1/miR-142-5p/DNMT1/maspin signaling pathway and may become a new idea for breast cancer diagnosis, treatment, and prognosis.

[1]  M. Primig,et al.  Nuclear translocation of MRTFA in MCF7 breast cancer cells shifts ERα nuclear/genomic to extra-nuclear/non genomic actions , 2021, Molecular and Cellular Endocrinology.

[2]  M. Haris,et al.  Epigenetic and Breast Cancer Therapy: Promising Diagnostic and Therapeutic Applications. , 2020, Seminars in cancer biology.

[3]  M. Karimzadeh,et al.  Regulation of DNA methylation machinery by epi-miRNAs in human cancer: emerging new targets in cancer therapy , 2020, Cancer Gene Therapy.

[4]  K. Wong.  DNMT1 as a therapeutic target in pancreatic cancer: mechanisms and clinical implications , 2020, Cellular Oncology.

[5]  Keng Wong Kah.  DNMT1: A Key Drug Target in Triple-Negative Breast Cancer. , 2020, Seminars in cancer biology.

[6]  G. MacLennan,et al.  Role of class I histone deacetylases in the regulation of maspin expression in prostate cancer , 2020, Molecular carcinogenesis.

[7]  Genfen Wang,et al.  MKL1/miR-5100/CAAP1 loop regulates autophagy and apoptosis in gastric cancer cells , 2020, Neoplasia.

[8]  Cheng‐Yi Chen,et al.  Radiosensitization of Hepatocellular Carcinoma through Targeting Radio-Associated MicroRNA , 2020, International journal of molecular sciences.

[9]  A. Meeker,et al.  Pervasive promoter hypermethylation of silenced TERT alleles in human cancers , 2020, Cellular Oncology.

[10]  S. Zanata,et al.  Identification of miRNAs Enriched in Extracellular Vesicles Derived from Serum Samples of Breast Cancer Patients , 2020, Biomolecules.

[11]  O. Bogdanović,et al.  Enhancer DNA methylation: implications for gene regulation. , 2019, Essays in biochemistry.

[12]  C. Vallot,et al.  High-throughput single-cell ChIP-seq identifies heterogeneity of chromatin states in breast cancer , 2019, Nature Genetics.

[13]  Xiangshu Jin,et al.  ERα is required for suppressing OCT4‐induced proliferation of breast cancer cells via DNMT1/ISL1/ERK axis , 2019, Cell proliferation.

[14]  Shou-Jiang Gao,et al.  Oncogenic KSHV-encoded interferon regulatory factor upregulates HMGB2 and CMPK1 expression to promote cell invasion by disrupting a complex lncRNA-OIP5-AS1/miR-218-5p network , 2019, PLoS pathogens.

[15]  A. Hoffman,et al.  A novel FLI1 exonic circular RNA promotes metastasis in breast cancer by coordinately regulating TET1 and DNMT1 , 2018, Genome Biology.

[16]  G. Botti,et al.  MicroRNA‐143‐3p inhibits growth and invasiveness of melanoma cells by targeting cyclooxygenase‐2 and inversely correlates with malignant melanoma progression , 2018, Biochemical pharmacology.

[17]  Hongbo Liu,et al.  Specific breast cancer prognosis‐subtype distinctions based on DNA methylation patterns , 2018, Molecular oncology.

[18]  Weixing Wang,et al.  MicroRNA-142-5p modulates breast cancer cell proliferation and apoptosis by targeting phosphatase and tensin homolog , 2018, Molecular medicine reports.

[19]  A. Vincent-Salomon,et al.  miR200-regulated CXCL12β promotes fibroblast heterogeneity and immunosuppression in ovarian cancers , 2018, Nature Communications.

[20]  C. Lengner,et al.  MiR-31 promotes mammary stem cell expansion and breast tumorigenesis by suppressing Wnt signaling antagonists , 2017, Nature Communications.

[21]  W. Guo,et al.  MiR‐93‐5p inhibits the EMT of breast cancer cells via targeting MKL‐1 and STAT3 , 2017, Experimental cell research.

[22]  S. Sheng,et al.  The Opportunity of Precision Medicine for Breast Cancer With Context‐Sensitive Tumor Suppressor Maspin , 2017, Journal of cellular biochemistry.

[23]  T. Ochiya,et al.  The role of extracellular vesicle microRNAs in cancer biology , 2017, Clinical chemistry and laboratory medicine.

[24]  S. Patra,et al.  DNA methylation and not H3K4 trimethylation dictates the expression status of miR-152 gene which inhibits migration of breast cancer cells via DNMT1/CDH1 loop. , 2016, Experimental cell research.

[25]  C. Heeschen,et al.  DNMT1 Inhibition Reprograms Pancreatic Cancer Stem Cells via Upregulation of the miR-17-92 Cluster. , 2016, Cancer research.

[26]  Z. Chang,et al.  The correlation between DNMT1 and ERα expression and the methylation status of ERα, and its clinical significance in breast cancer , 2016, Oncology letters.

[27]  K. Emmons,et al.  Study protocol for Young & Strong: a cluster randomized design to increase attention to unique issues faced by young women with newly diagnosed breast cancer , 2015, BMC Public Health.

[28]  M. Filip,et al.  A Comprehensive View of the Epigenetic Landscape. Part II: Histone Post-translational Modification, Nucleosome Level, and Chromatin Regulation by ncRNAs , 2014, Neurotoxicity Research.

[29]  Susan R. Quinn,et al.  The Role of Ets2 Transcription Factor in the Induction of MicroRNA-155 (miR-155) by Lipopolysaccharide and Its Targeting by Interleukin-10* , 2013, The Journal of Biological Chemistry.

[30]  D. Dhawan,et al.  DNMT1: an emerging target in the treatment of invasive urinary bladder cancer. , 2013, Urologic oncology.

[31]  S. Orkin,et al.  Corepressor-dependent silencing of fetal hemoglobin expression by BCL11A , 2013, Proceedings of the National Academy of Sciences.

[32]  Qing Xu,et al.  A regulatory circuit of miR-148a/152 and DNMT1 in modulating cell transformation and tumor angiogenesis through IGF-IR and IRS1. , 2013, Journal of molecular cell biology.

[33]  Hiromu Suzuki,et al.  DNA methylation and microRNA dysregulation in cancer , 2012, Molecular oncology.

[34]  M. Parmacek.  Myocardin-related transcription factor-A: mending a broken heart. , 2010, Circulation research.

[35]  A. Mackay-Sim,et al.  EdU, a new thymidine analogue for labelling proliferating cells in the nervous system , 2009, Journal of Neuroscience Methods.

[36]  Heather Fawcett,et al.  A rapid non-radioactive technique for measurement of repair synthesis in primary human fibroblasts by incorporation of ethynyl deoxyuridine (EdU) , 2008, Nucleic acids research.

[37]  S. Lau,et al.  5-Aza-2′-deoxycytidine-mediated reductions in G9A histone methyltransferase and histone H3 K9 di-methylation levels are linked to tumor suppressor gene reactivation , 2007, Oncogene.

[38]  W. Dean,et al.  DNA methylation in mammalian development and disease. , 2005, Birth defects research. Part C, Embryo today : reviews.

[39]  A. Hartmann,et al.  Elevated nuclear maspin expression is associated with microsatellite instability and high tumour grade in colorectal cancer , 2005, The Journal of pathology.