Reliable cloning of functional antibody variable domains from hybridomas and spleen cell repertoires employing a reengineered phage display system.

[1]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[2]  D. Desplancq,et al.  Selectively-infective phage (SIP): a mechanistic dissection of a novel in vivo selection for protein-ligand interactions. , 1997, Journal of molecular biology.

[3]  J. McCafferty,et al.  Antibody engineering: a practical approach , 1997 .

[4]  R. Zinkernagel,et al.  Monovalent single‐chain Fv fragments and bivalent miniantibodies bound to vesicular stomatitis virus protect against lethal infection , 1996, European journal of immunology.

[5]  J. Kwak,et al.  Cloning and characterization of cDNAs coding for heavy and light chains of a monoclonal antibody (MabB23) specific for human plasma apolipoprotein B-100. , 1996, Gene.

[6]  H. Michel,et al.  Improved cloning of antibody variable regions from hybridomas by an antisense-directed RNase H digestion of the P3-X63-Ag8.653 derived pseudogene mRNA. , 1996, Nucleic acids research.

[7]  Tristan J. Vaughan,et al.  Human Antibodies with Sub-nanomolar Affinities Isolated from a Large Non-immunized Phage Display Library , 1996, Nature Biotechnology.

[8]  C. Berger,et al.  Spectroscopic, calorimetric, and kinetic demonstration of conformational adaptation in peptide-antibody recognition. , 1995, Biochemistry.

[9]  A. Plückthun,et al.  Co‐selection of cognate antibody‐antigen pairs by selectively‐infective phages , 1995, FEBS letters.

[10]  D R Burton,et al.  CDR walking mutagenesis for the affinity maturation of a potent human anti-HIV-1 antibody into the picomolar range. , 1995, Journal of molecular biology.

[11]  C. Barbas,et al.  Synthetic Human Antibodies: Selecting and Evolving Functional Proteins , 1995 .

[12]  J. Engberg,et al.  pFab60: a new, efficient vector for expression of antibody Fab fragments displayed on phage. , 1995, Protein engineering.

[13]  R. Ames,et al.  Neutralizing murine monoclonal antibodies to human IL-5 isolated from hybridomas and a filamentous phage Fab display library. , 1995, Journal of immunology.

[14]  C. E. Miller,et al.  A murine antibody to Shigella dysenteriae type 1 employs V-genes that contain a rearranged codon for the lambda light chain. , 1995, Molecular immunology.

[15]  H. Ohmoto,et al.  An improved phage display antibody cloning system using newly designed PCR primers optimized for Pfu DNA polymerase. , 1995, Journal of biochemistry.

[16]  S. Halford,et al.  The SfiI restriction endonuclease makes a four-strand DNA break at two copies of its recognition sequence. , 1995, Journal of molecular biology.

[17]  J. Jackson,et al.  In vitro antibody maturation. Improvement of a high affinity, neutralizing antibody against IL-1 beta. , 1995, Journal of immunology.

[18]  A. Plückthun,et al.  Tetravalent miniantibodies with high avidity assembling in Escherichia coli. , 1995, Journal of molecular biology.

[19]  J. Chauveau,et al.  Paratope characterization by structural modelling of two anti-cortisol single-chain variable fragments produced in E. coli. , 1995, Molecular immunology.

[20]  H. Michel,et al.  Crystals of an antibody FV fragment against an integral membrane protein diffracting to 1.28 Å resolution , 1995, Proteins.

[21]  A. Plückthun,et al.  An improved affinity tag based on the FLAG peptide for the detection and purification of recombinant antibody fragments. , 1994, BioTechniques.

[22]  A. Lawson,et al.  Multimerization behaviour of single chain Fv variants for the tumour-binding antibody B72.3. , 1994, Protein engineering.

[23]  Thomas Mr.  Simple, effective cleanup of DNA ligation reactions prior to electro-transformation of E. coli. , 1994 .

[24]  I. Pastan,et al.  Stabilization of the Fv fragments in recombinant immunotoxins by disulfide bonds engineered into conserved framework regions. , 1994, Biochemistry.

[25]  D. Webster,et al.  Antibody design: beyond the natural limits. , 1994, Trends in biotechnology.

[26]  S. Benkovic,et al.  Catalytic antibodies: perusing combinatorial libraries. , 1994, Trends in biochemical sciences.

[27]  K. Ansell,et al.  Isolation of tumor cell‐specific single‐chain Fv from immunized mice using phage‐antibody libraries and the re‐construction of whole antibodies from these antibody fragments , 1994, European journal of immunology.

[28]  C. MacKenzie,et al.  Selection of antibody single-chain variable fragments with improved carbohydrate binding by phage display. , 1994, The Journal of biological chemistry.

[29]  T. Holak,et al.  Structural and dynamic properties of the Fv fragment and the single-chain Fv fragment of an antibody in solution investigated by heteronuclear three-dimensional NMR spectroscopy. , 1994, Biochemistry.

[30]  R. Fisher,et al.  Optimization of primer sequences for mouse scFv repertoire display library construction. , 1994, Nucleic acids research.

[31]  A. Plückthun,et al.  Improved Bivalent Miniantibodies, with Identical Avidity as Whole Antibodies, Produced by High Cell Density Fermentation of Escherichia coli , 1993, Nature Biotechnology.

[32]  V. G. Johnson,et al.  An improved method for generating single-chain antibodies from hybridomas. , 1993, Journal of immunological methods.

[33]  P. S. Andersen,et al.  Efficient method for constructing comprehensive murine Fab antibody libraries displayed on phage. , 1993, Nucleic acids research.

[34]  L. Riechmann,et al.  Phage display and selection of a site-directed randomized single-chain antibody Fv fragment for its affinity improvement. , 1993, Biochemistry.

[35]  J. Saldanha,et al.  Optimization of primers for cloning libraries of mouse immunoglobulin genes using the polymerase chain reaction , 1993, European journal of immunology.

[36]  U. Weidle,et al.  A general method for chimerization of monoclonal antibodies by inverse polymerase chain reaction which conserves authentic N-terminal sequences. , 1992, Gene.

[37]  Andrew D. Griffiths,et al.  By–Passing Immunization: Building High Affinity Human Antibodies by Chain Shuffling , 1992, Bio/Technology.

[38]  C. Milstein,et al.  Original and artificial antibodies , 1992, Nature.

[39]  R. Mocikat,et al.  Rapid isolation of immunoglobulin variable genes from cell lysates of rat hybridomas by polymerase chain reaction. , 1992, Hybridoma.

[40]  H R Hoogenboom,et al.  By-passing immunization. Human antibodies from V-gene libraries displayed on phage. , 1991, Journal of molecular biology.

[41]  S. Bass,et al.  Selecting high-affinity binding proteins by monovalent phage display. , 1991, Biochemistry.

[42]  C. Barbas,et al.  Assembly of combinatorial antibody libraries on phage surfaces: the gene III site. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[43]  T. Clackson,et al.  Making antibody fragments using phage display libraries , 1991, Nature.

[44]  H R Hoogenboom,et al.  Multi-subunit proteins on the surface of filamentous phage: methodologies for displaying antibody (Fab) heavy and light chains. , 1991, Nucleic acids research.

[45]  C. Barbas,et al.  Linkage of recognition and replication functions by assembling combinatorial antibody Fab libraries along phage surfaces. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[46]  G. Winter,et al.  Phage antibodies: filamentous phage displaying antibody variable domains , 1990, Nature.

[47]  D. De Bellis,et al.  Regulated expression of foreign genes fused to lac: control by glucose levels in growth medium. , 1990, Nucleic acids research.

[48]  P. Bross,et al.  Dissection of functional domains in phage fd adsorption protein. Discrimination between attachment and penetration sites. , 1990, Journal of molecular biology.

[49]  B. Gutte,et al.  Preparation and characterization of polyclonal and monoclonal antibodies against the insecticide DDT. , 1990, Biochemical and biophysical research communications.

[50]  R. Glockshuber,et al.  A comparison of strategies to stabilize immunoglobulin Fv-fragments. , 1990, Biochemistry.

[51]  G. Winter,et al.  Cloning immunoglobulin variable domains for expression by the polymerase chain reaction. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[52]  S. Ho,et al.  Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension. , 1989, Gene.

[53]  S. Levy,et al.  Hybridoma fusion cell lines contain an aberrant kappa transcript. , 1988, Molecular immunology.

[54]  W. J. Dower,et al.  High efficiency transformation of E. coli by high voltage electroporation , 1988, Nucleic Acids Res..

[55]  R. Souhami,et al.  Chapter 1 The first international workshop on small cell lung cancer antigens , 1988 .

[56]  S. Weiss,et al.  Somatic point mutations in unrearranged immunoglobulin gene segments encoding the variable region of lambda light chains. , 1987, The EMBO journal.

[57]  G. Kroemer,et al.  Complete variable region sequence of a nonfunctionally rearranged kappa light chain transcribed in the nonsecretor P3-X63-Ag8.653 myeloma cell line. , 1987, Nucleic acids research.

[58]  J. Taylor,et al.  Sequence of the gene for alkaline phosphatase from Escherichia coli JM83. , 1986, Nucleic acids research.

[59]  S. Munro,et al.  An hsp70-like protein in the ER: Identity with the 78 kd glucose-regulated protein and immunoglobulin heavy chain binding protein , 1986, Cell.

[60]  K. Rajewsky,et al.  A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines. , 1979, Journal of immunology.

[61]  C. Milstein,et al.  Derivation of specific antibody‐producing tissue culture and tumor lines by cell fusion , 1976, European journal of immunology.

[62]  E. Kremmer,et al.  Specific detection of his-tagged proteins with recombinant anti-His tag scFv-phosphatase or scFv-phage fusions. , 1997, BioTechniques.

[63]  A. Plückthun,et al.  Inclusion of an upstream transcriptional terminator in phage display vectors abolishes background expression of toxic fusions with coat protein g3p. , 1996, Gene.

[64]  C. Borrebaeck,et al.  Light chain shuffling of a high affinity antibody results in a drift in epitope recognition. , 1996, Molecular immunology.

[65]  A. Plückthun,et al.  Engineered turns of a recombinant antibody improve its in vivo folding. , 1995, Protein engineering.

[66]  J. Schlager,et al.  A phage display vector with improved stability, applicability and ease of manipulation. , 1995, Gene.

[67]  P. S. Andersen,et al.  Phage display used for gene cloning of human recombinant antibody against the erythrocyte surface antigen, rhesus D. , 1995, Journal of immunological methods.

[68]  G. Winter,et al.  Making antibodies by phage display technology. , 1994, Annual review of immunology.

[69]  L. Duan,et al.  Elimination of endogenous aberrant kappa chain transcripts from sp2/0-derived hybridoma cells by specific ribozyme cleavage: utility in genetic therapy of HIV-1 infections. , 1994, Nucleic acids research.

[70]  M. R. Thomas.  Simple, effective cleanup of DNA ligation reactions prior to electro-transformation of E. coli. , 1994, BioTechniques.

[71]  J. Huston,et al.  Medical applications of single-chain antibodies. , 1993, International reviews of immunology.

[72]  P. Lindner.  Purification of native proteins from the cytoplasm and periplasm of Escherichia coli using IMAC and histidine tails: A comparison of proteins and protocols , 1992 .

[73]  A. Zelenetz.  Construction of complex directional complementary DNA libraries in SfiI. , 1992, Methods in enzymology.

[74]  D. Güssow,et al.  Humanization of monoclonal antibodies. , 1991, Methods in enzymology.

[75]  E. Kabat,et al.  Sequences of proteins of immunological interest , 1991 .

[76]  K. Fry,et al.  Recombinant antibodies. , 1991, Human antibodies and hybridomas.

[77]  J. Chirgwin,et al.  Isolation of RNA. , 1989, Methods in enzymology.

[78]  M. Ashburner.  A Laboratory manual , 1989 .

[79]  A. Riggs,et al.  Immunoglobulin transcripts and molecular history of a hybridoma that produces antibody to carcinoembryonic antigen. , 1985, Gene.