Zymography methods for visualizing hydrolytic enzymes

[1]  B. Cravatt,et al.  Activity-Based Labeling of Matrix Metalloproteinases in Living Vertebrate Embryos , 2012, PloS one.

[2]  F. Van Breusegem,et al.  Natural substrates of plant proteases: how can protease degradomics extend our knowledge? , 2012, Physiologia plantarum.

[3]  Regent Lee Letter by Lee regarding article, "Matrix metalloproteinase-9 in an exploratory trial of intravenous minocycline for acute stroke". , 2011, Stroke.

[4]  A. Miller-Larsson,et al.  TGFβ-induced matrix production by bronchial fibroblasts in asthma: budesonide and formoterol effects. , 2011, Respiratory Medicine.

[5]  P. Riccio,et al.  2-D zymographic analysis of Broccoli (Brassica oleracea L. var. Italica) florets proteases: follow up of cysteine protease isotypes in the course of post-harvest senescence. , 2011, Journal of plant physiology.

[6]  G. Verleden,et al.  Azithromycin decreases MMP-9 expression in the airways of lung transplant recipients. , 2011, Transplant immunology.

[7]  J. Switzer,et al.  Matrix Metalloproteinase-9 in an Exploratory Trial of Intravenous Minocycline for Acute Ischemic Stroke , 2011, Stroke.

[8]  N. Lu,et al.  Inhibitory effects of GL-V9 on the invasion of human breast carcinoma cells by downregulating the expression and activity of matrix metalloproteinase-2/9. , 2011, European Journal of Pharmaceutical Sciences.

[9]  D. Nakayama,et al.  Interleukin-lβ induces migration of rat arterial smooth muscle cells through a mechanism involving increased matrix metalloproteinase-2 activity. , 2011, The Journal of surgical research.

[10]  C. Egas,et al.  Expression of genes encoding extracellular matrix macromolecules and metalloproteinases in avian tibial dyschondroplasia. , 2011, Journal of comparative pathology.

[11]  J. del Valle,et al.  Role of matrix metalloproteinase‐9 (MMP‐9) in striatal blood–brain barrier disruption in a 3‐nitropropionic acid model of Huntington's disease , 2011, Neuropathology and applied neurobiology.

[12]  V. Belo,et al.  Consistent alterations of circulating matrix metalloproteinases levels in untreated hypertensives and in spontaneously hypertensive rats: a relevant pharmacological target. , 2011, Basic & clinical pharmacology & toxicology.

[13]  B. Klanke,et al.  Effect of the plasminogen-plasmin system on hypertensive renal and cardiac damage , 2011, Journal of hypertension.

[14]  Wan‐Lin Wu,et al.  Advanced glycation end products cause collagen II reduction by activating Janus kinase/signal transducer and activator of transcription 3 pathway in porcine chondrocytes. , 2011, Rheumatology.

[15]  J. Connor,et al.  Increased cellular iron levels affect matrix metalloproteinase expression and phagocytosis in activated microglia , 2011, Neuroscience Letters.

[16]  A. Hill,et al.  Electrophoretic transfer protein zymography. , 2011, Analytical biochemistry.

[17]  B. Yoon,et al.  Silver-stained fibrin zymography: separation of proteases and activity detection using a single substrate-containing gel , 2011, Biotechnology Letters.

[18]  B. D. Crawford,et al.  Differential in vivo zymography: a method for observing matrix metalloproteinase activity in the zebrafish embryo. , 2011, Matrix biology : journal of the International Society for Matrix Biology.

[19]  D. Hahm,et al.  Synthesis activity-based zymography for detection of lipases and esterases , 2011, Biotechnology Letters.

[20]  P. Herdewijn,et al.  Gelatin degradation assay reveals MMP-9 inhibitors and function of O-glycosylated domain. , 2011, World journal of biological chemistry.

[21]  N. Nagai,et al.  Profibrinolytic effect of Enzamin, an extract of metabolic products from Bacillus subtilis AK and Lactobacillus , 2011, Journal of Thrombosis and Thrombolysis.

[22]  B. Cauwe,et al.  Intracellular substrate cleavage: a novel dimension in the biochemistry, biology and pathology of matrix metalloproteinases , 2010, Critical reviews in biochemistry and molecular biology.

[23]  E. V. Van Bockstaele,et al.  HIV-1 gp120-Induced Injury to the Blood-Brain Barrier: Role of Metalloproteinases 2 and 9 and Relationship to Oxidative Stress , 2010, Journal of neuropathology and experimental neurology.

[24]  M. Tien,et al.  Identification of proteins involved in lignocellulose degradation using in gel zymogram analysis combined with mass spectroscopy‐based peptide analysis of gut proteins from larval Asian longhorned beetles, Anoplophora glabripennis , 2010 .

[25]  Amy E Herr,et al.  Quantitative enzyme activity determination with zeptomole sensitivity by microfluidic gradient-gel zymography. , 2010, Analytical chemistry.

[26]  P. Ferdinandy,et al.  Matrix metalloproteinase activity assays: Importance of zymography. , 2010, Journal of pharmacological and toxicological methods.

[27]  M. Terol,et al.  Matrix metalloproteinase-9 promotes chronic lymphocytic leukemia b cell survival through its hemopexin domain. , 2010, Cancer cell.

[28]  B. D. Crawford,et al.  The zebrafish embryo: a powerful model system for investigating matrix remodeling. , 2009, Zebrafish.

[29]  J. Wilkesman,et al.  Protease analysis by zymography: a review on techniques and patents. , 2009, Recent patents on biotechnology.

[30]  P. E. Van den Steen,et al.  “Reverse degradomics”, monitoring of proteolytic trimming by multi‐CE and confocal detection of fluorescent substrates and reaction products , 2009, Electrophoresis.

[31]  Peng Tao,et al.  DFT studies of the ring-opening mechanism of SB-3CT, a potent inhibitor of matrix metalloproteinase 2. , 2009, Organic Letters.

[32]  Joong-Su Kim,et al.  Mixed‐substrate (glycerol tributyrate and fibrin) zymography for simultaneous detection of lipolytic and proteolytic enzymes on a single gel , 2009, Electrophoresis.

[33]  J. Lakritz,et al.  Kinetics of inhibition of purified bovine neutrophil matrix metalloproteinase 9 by low-molecular-weight inhibitors. , 2009, American journal of veterinary research.

[34]  J. Song,et al.  Multiple-layer substrate zymography for detection of several enzymes in a single sodium dodecyl sulfate gel. , 2009, Analytical biochemistry.

[35]  W. Dietrich Aprotinin: 1 year on , 2009, Current opinion in anaesthesiology.

[36]  Oliver Schilling,et al.  Proteome-derived, database-searchable peptide libraries for identifying protease cleavage sites , 2008, Nature Biotechnology.

[37]  L. Matrisian,et al.  Optical Imaging of Matrix Metalloproteinase-7 Activity in Vivo Using a Proteolytic Nanobeacon , 2008, Molecular imaging.

[38]  V. Labas,et al.  One- and two-dimensional SDS-PAGE zymography with quenched fluorogenic substrates provides identification of biological fluid proteases by direct mass spectrometry. , 2008, Analytical biochemistry.

[39]  J. Tanus-Santos,et al.  Silicate increases the release of MMP-9 forms in peripheral blood: why gelatin zymography differs significantly in citrate plasma and serum obtained with or without clot activators. , 2007, Clinical chemistry.

[40]  Caitlin Smith Keeping tabs on fluorescent tags , 2007, Nature Methods.

[41]  C. Löwik,et al.  Whole-Body Optical Imaging in Animal Models to Assess Cancer Development and Progression , 2007, Clinical Cancer Research.

[42]  Jialiang Hu,et al.  Matrix metalloproteinase inhibitors as therapy for inflammatory and vascular diseases , 2007, Nature Reviews Drug Discovery.

[43]  Christopher M Overall,et al.  Proteomics Discovery of Metalloproteinase Substrates in the Cellular Context by iTRAQ™ Labeling Reveals a Diverse MMP-2 Substrate Degradome*S , 2007, Molecular & Cellular Proteomics.

[44]  G. Opdenakker,et al.  Matrix metalloproteinases, tissue inhibitors of MMPs and TACE in experimental cerebral malaria , 2006, Laboratory Investigation.

[45]  R. Dwek,et al.  The Hemopexin and O-Glycosylated Domains Tune Gelatinase B/MMP-9 Bioavailability via Inhibition and Binding to Cargo Receptors* , 2006, Journal of Biological Chemistry.

[46]  G. Opdenakker,et al.  Dystroglycan is selectively cleaved at the parenchymal basement membrane at sites of leukocyte extravasation in experimental autoimmune encephalomyelitis , 2006, The Journal of experimental medicine.

[47]  D. Pilgrim,et al.  Ontogeny and regulation of matrix metalloproteinase activity in the zebrafish embryo by in vitro and in vivo zymography. , 2005, Developmental biology.

[48]  F. Ibarra-Velarde,et al.  Fasciola hepatica proteolytic activity in liver revealed by in situ zymography , 2005, Parasitology Research.

[49]  R. Weissleder,et al.  Optical zymography for specific detection of urokinase plasminogen activator activity in biological samples. , 2005, Analytical biochemistry.

[50]  B. Nielsen,et al.  Detection of plasminogen activators in oral cancer by laser capture microdissection combined with zymography. , 2004, Oral oncology.

[51]  R. Harrison,et al.  Antibody zymography: a novel adaptation of zymography to determine the protease-neutralising potential of specific antibodies and snake antivenoms. , 2004, Journal of immunological methods.

[52]  Nasreen S Jessani,et al.  Activity-based probes for the proteomic profiling of metalloproteases. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[53]  P. Maeng,et al.  Nano-scale proteomics approach using two-dimensional fibrin zymography combined with fluorescent SYPRO ruby dye. , 2004, Journal of biochemistry and molecular biology.

[54]  X. Puente,et al.  Protease degradomics: mass spectrometry discovery of protease substrates and the CLIP-CHIP, a dedicated DNA microarray of all human proteases and inhibitors , 2004, Biological chemistry.

[55]  T. Schirmeister,et al.  Cysteine protease inhibitors containing small rings. , 2003, Mini reviews in medicinal chemistry.

[56]  V. Kaberdin,et al.  Expanding the use of zymography by the chemical linkage of small, defined substrates to the gel matrix. , 2003, Genome research.

[57]  E. Blomme,et al.  In Situ Zymography: A Molecular Pathology Technique to Localize Endogenous Protease Activity in Tissue Sections , 2003, Veterinary pathology.

[58]  F. Mannello Effects of blood collection methods on gelatin zymography of matrix metalloproteinases. , 2003, Clinical chemistry.

[59]  P. Gettins Serpin structure, mechanism, and function. , 2002, Chemical reviews.

[60]  G. Opdenakker,et al.  Analysis of Gelatinases in Complex Biological Fluids and Tissue Extracts , 2002, Laboratory Investigation.

[61]  C. López-Otín,et al.  Protease degradomics: A new challenge for proteomics , 2002, Nature Reviews Molecular Cell Biology.

[62]  J. Gatti,et al.  Comparison, Characterization, and Identification of Proteases and Protease Inhibitors in Epididymal Fluids of Domestic Mammals. Matrix Metalloproteinases Are Major Fluid Gelatinases1 , 2002, Biology of reproduction.

[63]  H. Hatch,et al.  Casein zymography of calpains using a 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid-imidazole buffer. , 2002, Analytical biochemistry.

[64]  S. Hattori,et al.  Real-time zymography and reverse zymography: a method for detecting activities of matrix metalloproteinases and their inhibitors using FITC-labeled collagen and casein as substrates. , 2002, Analytical biochemistry.

[65]  Seung Ho Kim,et al.  A functional proteomic analysis of secreted fibrinolytic enzymes from Bacillus subtilis 168 using a combined method of two‐dimensional gel electrophoresis and zymography , 2002, Proteomics.

[66]  Pauline M. Rudd,et al.  Biochemistry and Molecular Biology of Gelatinase B or Matrix Metalloproteinase-9 (MMP-9) , 2002, Critical reviews in biochemistry and molecular biology.

[67]  Ralph Weissleder,et al.  In vivo molecular target assessment of matrix metalloproteinase inhibition , 2001, Nature Medicine.

[68]  W. Linss,et al.  Zymography with caseogram prints: quantification of pepsinogen. , 2001, Analytical biochemistry.

[69]  J. Geesin,et al.  Incorporation of fluorescent enzyme substrates in agarose gel for in situ zymography. , 2001, Analytical biochemistry.

[70]  C. Pollitt,et al.  In situ zymography: topographical considerations. , 2001, Journal of Biochemical and Biophysical Methods.

[71]  Y. Tamura,et al.  Inhibition of gelatinolytic activity in tumor tissues by synthetic matrix metalloproteinase inhibitor: application of film in situ zymography. , 2000, Clinical cancer research : an official journal of the American Association for Cancer Research.

[72]  H. Welgus,et al.  A versatile assay for gelatinases using succinylated gelatin. , 2000, Matrix biology : journal of the International Society for Matrix Biology.

[73]  L. Kotra,et al.  Potent and Selective Mechanism-Based Inhibition of Gelatinases , 2000 .

[74]  S. H. Kim,et al.  Two fibrin zymography methods for analysis of plasminogen activators on gels. , 2000, Analytical biochemistry.

[75]  J. Perrot,et al.  Quantitative zymography of matrix metalloproteinases by measuring hydroxyproline: Application to gelatinases A and B , 1999, Electrophoresis.

[76]  Z. Werb,et al.  Matrix Metalloproteinase-9/Gelatinase B Is Required for Process Outgrowth by Oligodendrocytes , 1999, The Journal of Neuroscience.

[77]  T. Kaku,et al.  In situ detection of gelatinolytic activity in developing craniofacial tissues , 1999, Anatomy and Embryology.

[78]  J. Quigley,et al.  Alpha2-macroglobulin: an evolutionarily conserved arm of the innate immune system. , 1999, Developmental and comparative immunology.

[79]  R. Weissleder,et al.  In vivo imaging of tumors with protease-activated near-infrared fluorescent probes , 1999, Nature Biotechnology.

[80]  A. Harris,et al.  Phase I study of intrapleural batimastat (BB-94), a matrix metalloproteinase inhibitor, in the treatment of malignant pleural effusions. , 1999, Clinical cancer research : an official journal of the American Association for Cancer Research.

[81]  Seung Ho Kim,et al.  Fibrin zymography: a direct analysis of fibrinolytic enzymes on gels. , 1998, Analytical biochemistry.

[82]  W. Hornebeck,et al.  Collagen zymography as a sensitive and specific technique for the determination of subpicogram levels of interstitial collagenase. , 1998, Analytical biochemistry.

[83]  E. Roets,et al.  The gelatinase inhibitory activity of tetracyclines and chemically modified tetracycline analogues as measured by a novel microtiter assay for inhibitors. , 1996, Biochemical pharmacology.

[84]  P. Libby,et al.  Microscopic localization of active proteases by in situ zymography: detection of matrix metalloproteinase activity in vascular tissue , 1995, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[85]  T. Lenarz,et al.  Ultrathin horizontal sodium dodecyl sulfate zymography. , 1995, Analytical Biochemistry.

[86]  S. Yamagata,et al.  Analysis of glycosaminoglycan-degrading enzymes by substrate gel electrophoresis (zymography). , 1995, Analytical biochemistry.

[87]  P. Libby,et al.  Increased expression of matrix metalloproteinases and matrix degrading activity in vulnerable regions of human atherosclerotic plaques. , 1994, The Journal of clinical investigation.

[88]  G. Opdenakker,et al.  Evaluation of gelatinases and IL-6 in the cerebrospinal fluid of patients with optic neuritis, multiple sclerosis and other inflammatory neurological diseases , 1994, Journal of Neuroimmunology.

[89]  W. Stetler-Stevenson,et al.  Quantitative zymography: detection of picogram quantities of gelatinases. , 1994, Analytical biochemistry.

[90]  M. Ingelman-Sundberg,et al.  Differential regulation of gelatinase b and tissue‐type plasminogen activator expression in human bowes melanoma cells , 1993, International journal of cancer.

[91]  L. Liotta,et al.  Urinary type IV collagenase: elevated levels are associated with bladder transitional cell carcinoma. , 1992, Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology.

[92]  R. Beynon,et al.  Synthetic analogues of chymostatin. Inhibition of chymotrypsin and Streptomyces griseus proteinase A. , 1992, Biochemical Journal.

[93]  G. M. Liuzzi,et al.  Identification of water-soluble proteases in myelin preparations. , 1992, Biochemical and biophysical research communications.

[94]  G. Opdenakker,et al.  Purification and identification of 91-kDa neutrophil gelatinase. Release by the activating peptide interleukin-8. , 1991, European journal of biochemistry.

[95]  G. Opdenakker,et al.  Human hepatoma cells produce an 85 kDa gelatinase regulated by phorbol 12-myristate 13-acetate. , 1990, Biochimica et biophysica acta.

[96]  N. Tarasova,et al.  Detection of proteases by clotting of casein after gel electrophoresis. , 1985, Analytical biochemistry.

[97]  Z. Werb,et al.  Purification and characterization of an elastinolytic proteinase secreted by cercariae of Schistosoma mansoni. , 1985, The Journal of biological chemistry.

[98]  J. Seyer,et al.  Biochemical and immunological characterization of the secreted forms of human neutrophil gelatinase. , 1985, The Journal of biological chemistry.

[99]  G. Opdenakker,et al.  Mechanism of the Stimulatory Effect of Phorbol 12-Myristate 13-Acetate on Cellular Production of Plasminogen Activator , 1984, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[100]  R. Beynon,et al.  Inhibition of hepatic protein degradation by synthetic analogues of chymostatin. , 1983, The Journal of biological chemistry.

[101]  D. Every Quantitative measurement of protease activities in slab polyacrylamide gel electrophoretograms. , 1981, Analytical biochemistry.

[102]  E. Dowdle,et al.  Electrophoretic analysis of plasminogen activators in polyacrylamide gels containing sodium dodecyl sulfate and copolymerized substrates. , 1980, Analytical biochemistry.

[103]  A. Granelli‐Piperno,et al.  A study of proteases and protease-inhibitor complexes in biological fluids , 1978, The Journal of experimental medicine.

[104]  J. Vassalli,et al.  Secretion of plasminogen activator by human polymorphonuclear leukocytes. Modulation by glucocorticoids and other effectors , 1977, The Journal of experimental medicine.

[105]  T. Aoyagi,et al.  Letter: Elastatinal, a new elastase inhibitor produced by actinomycetes. , 1973, The Journal of antibiotics.

[106]  J. Dingle,et al.  The inhibition of tissue acid proteinases by pepstatin. , 1972, The Biochemical journal.

[107]  F. Markwardt,et al.  Comparative studies on the inhibition of trypsin, plasmin, and thrombin by derivatives of benzylamine and benzamidine. , 1968, European journal of biochemistry.

[108]  C. Lapière,et al.  Collagenolytic activity in amphibian tissues: a tissue culture assay. , 1962, Proceedings of the National Academy of Sciences of the United States of America.

[109]  J. W. Von den Hoff,et al.  Zymographic techniques for the analysis of matrix metalloproteinases and their inhibitors. , 2005, BioTechniques.

[110]  W. Stetler-Stevenson,et al.  Quantitative reverse zymography: analysis of picogram amounts of metalloproteinase inhibitors using gelatinase A and B reverse zymograms. , 1997, Analytical biochemistry.

[111]  G. Opdenakker On the roles of extracellular matrix remodeling by gelatinase B. , 1997, Verhandelingen - Koninklijke Academie voor Geneeskunde van Belgie.

[112]  I. Talbot,et al.  Matrix metalloprotease 2 (MMP-2) and matrix metalloprotease 9 (MMP-9) type IV collagenases in colorectal cancer. , 1996, Cancer research.

[113]  A. R. Penheiter,et al.  Anomalous estimation of protease molecular weights using gelatin-containing Sds-Page. , 1996, Analytical biochemistry.