Identification and determination of the flavonoids from Ginkgo biloba by high-performance liquid chromatography

Abstract Qualitative and quantitative reversed-phase high-performance liquid chromatographic methods have been developed for the separation and determination of the flavonoids found in the leaves and therapeutically used extracts of Ginkgo biloba. The first method includes hydrolysis of the flavonoids and subsequent quantitative chromatographic assay of the obtained aglycones and qualitative analysis of the biflavones. The second method is a “fingerprint” procedure to identify unambiguously 33 flavonoids of Ginkgo biloba.