Serological diagnosis of acute delta hepatitis

Sixty‐three intravenous drug addicts with HBsAg positive hepatitis were studied to evaluate the diagnostic usefulness of hepatitis delta virus (HDV) markers for diagnosis of acute HDV infection. Patients were tested for HBsAg, anti‐HBc‐IgM, and anti‐HD‐IgM by radioimmunoassay (RIA), and for hepatitis delta antigen (HD‐Ag) by a commercial enzyme‐linked immunoassay (ELISA). At least two serum samples at a mean interval of 4 wk were examined from each patient. HDV markers were found in 41 cases. In the first serum sample (obtained within 1–5 wk after onset of illness) HD‐Ag was found in 32 cases and was the only HDV marker in 22; in the remaining 10 cases, HD‐Ag was found along with total anti‐HD, and in 6 of them anti‐HD‐IgM was also detected. Five additional patients were only positive for total anti‐HD, and anti‐HD of the IgM class was the only marker in one patient. HD‐Ag was found more often in the patients studied during the first 2 wk of illness.

[1]  H. Thomas.  The delta agent comes of age. , 1985, Gut.

[2]  G. Raimondo,et al.  Delta agent infection in acute hepatitis and chronic HBsAg carriers with and without liver disease. , 1984, Gut.

[3]  I. Gust,et al.  Delta agent infection in melbourne , 1984, Journal of medical virology.

[4]  A. Shattock,et al.  Sensitive enzyme immunoassay for the detection of delta antigen and anti‐delta, Using serum as the delta antigen source , 1984, Journal of medical virology.

[5]  M. Rizzetto,et al.  The Delta Agent , 2007, Hepatology.

[6]  N. Caporaso,et al.  Delta hepatitis in inapparent carriers of hepatitis B surface antigen. A disease simulating acute hepatitis B progressive to chronicity. , 1983, Gastroenterology.

[7]  J. Peutherer,et al.  HIGH INCIDENCE OF DELTA ANTIGEN IN SERUM , 1983, The Lancet.

[8]  M. Rizzetto,et al.  Epidemiologic patterns of infection with the hepatitis B virus-associated delta agent in Italy. , 1983, American journal of epidemiology.

[9]  A. Widell,et al.  Infection with delta agent in Sweden: introduction of a new hepatitis agent. , 1982, The Journal of infectious diseases.

[10]  G. Raimondo,et al.  Radioimmunoassay detection of igm antibodies to the hbv‐associated delta (δ) antigen: Clinical significance in δ infection , 1982 .

[11]  M. Rizzetto,et al.  Enzyme-linked immunosorbent assay for detection of antibody to the hepatitis B surface antigen-associated delta antigen , 1981, Journal of clinical microbiology.

[12]  M. Rizzetto,et al.  The hepatitis B virus-associated delta antigen: isolation from liver, development of solid-phase radioimmunoassays for delta antigen and anti-delta and partial characterization of delta antigen. , 1980, Journal of immunology.

[13]  R. Purcell,et al.  INCIDENCE AND SIGNIFICANCE OF ANTIBODIES TO DELTA ANTIGEN IN HEPATITIS B VIRUS INFECTION , 1979, The Lancet.

[14]  P. Mccormack.  BUMETANIDE IN RENAL FAILURE , 1976, The Lancet.

[15]  J. Mackeith.  DISCHARGE OF VIOLENT PATIENTS , 1975, The Lancet.