The determination of phospholipids in middle ear effusion from experimental otitis media in the chinchilla.

Phospholipid types were clarified both in eustachian tube secretion (ETS) and middle ear effusions (MEEs) from chinchilla middle ear. ETS was obtained from normal healthy animals and from animals with experimentally induced purulent otitis media (POM). MEE was obtained from experimentally induced serous and purulent otitis media (SOM and POM). In normal ETS, phosphatidylcholine (36.4%) was predominant followed by phosphatidylethanolamine (30.6%), sphingomyelin (18.0%), phosphatidylinositol (7.2%), phosphatidylglycerol (8.8%) and phosphatidylserine (2.5%). A significant loss of phosphatidylcholine was observed in ETS and MEE from experimentally induced otitis media. These findings suggest that both POM and SOM might be caused by a relative decrease of phospholipids, which is a major component of surfactants. Furthermore, the analysis of phospholipids in MEE may yield baseline information necessary to specify the factors responsible for chronicity or the tendency of recurrence of otitis media.

[1]  T. Kumazawa,et al.  Ultracytochemical demonstration of phospholipids in the surface layer of the guinea pig eustachian tube. , 1989, Acta oto-laryngologica.

[2]  M. Iwamori,et al.  Biochemical Characterization of Middle Ear Effusions from Children and Adult Patients , 1989 .

[3]  T. Brask,et al.  Secretory otitis media--a question of surface activity in the eustachian tube? , 1988, Acta oto-laryngologica.

[4]  A. Grace,et al.  Surfactant in Middle Ear Effusions , 1987, Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery.

[5]  D. Stokes,et al.  Surfactant phospholipids and lavage phospholipase A2 in experimental Pneumocystis carinii pneumonia. , 1986, The American review of respiratory disease.

[6]  B. Hills.  Hydrophobic lining of the eustachian tube imparted by surfactant. , 1984, Archives of otolaryngology.

[7]  R. Lumb,et al.  Rat eustachian tube synthesizes disaturated phosphatidylcholine. , 1984, Biochimica et biophysica acta.

[8]  B. Hills.  Analysis of eustachian surfactant and its function as a release agent. , 1984, Archives of otolaryngology.

[9]  J. Masliah,et al.  Phospholipase activities of bronchoalveolar lavage fluid in rat Pneumocystis carinii pneumonia. , 1983, British journal of experimental pathology.

[10]  R. Spragg,et al.  Evidence of lung surfactant abnormality in respiratory failure. Study of bronchoalveolar lavage phospholipids, surface activity, phospholipase activity, and plasma myoinositol. , 1982, The Journal of clinical investigation.

[11]  G. S. Patil,et al.  Surface-Active Substances of the Guinea Pig Tubotympanum: A Chemical and Physical Analysis , 1981, Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery.

[12]  S. Juhn,et al.  Pathogenesis of Otitis Media , 1977, The Annals of otology, rhinology, and laryngology.

[13]  D. Lim,et al.  Functional Morphology of the Lining Membrane of the Middle Ear and Eustachian Tube , 1974, The Annals of otology, rhinology, and laryngology.

[14]  K. Brookler,et al.  Surface Tension Lowering Substance of the Canine Eustachian Tube , 1972, The Annals of otology, rhinology, and laryngology.

[15]  F. Bauer.  The use of urea in exudative otitis media. , 1970, Otolaryngologic clinics of North America.

[16]  H. Perlman.  Normal tubal function. , 1967, Archives of otolaryngology.

[17]  S. Ingelstedt,et al.  The valve and "locking" mechanisms of the eustachian tube. , 1963, Acta oto-laryngologica. Supplementum.

[18]  W. J. Dyer,et al.  A rapid method of total lipid extraction and purification. , 1959, Canadian journal of biochemistry and physiology.

[19]  R. E. Pattle,et al.  Properties, Function and Origin of the Alveolar Lining Layer , 1955, Nature.

[20]  L. F. Edwards,et al.  THE EUSTACHIAN TUBE: A REVIEW OF ITS DESCRIPTIVE, MICROSCOPIC, TOPOGRAPHIC AND CLINICAL ANATOMY , 1944 .