Rational designed mutagenesis of levansucrase from Bacillus licheniformis 8-37-0-1 for product specificity study

[1]  D. Sheu,et al.  Neokestose suppresses the growth of human melanoma A2058 cells via inhibition of the nuclear factor‑κB signaling pathway. , 2017, Molecular medicine reports.

[2]  L. Hernández,et al.  Review of Levan polysaccharide: From a century of past experiences to future prospects. , 2016, Biotechnology advances.

[3]  M. Costas,et al.  Size product modulation by enzyme concentration reveals two distinct levan elongation mechanisms in Bacillus subtilis levansucrase. , 2016, Glycobiology.

[4]  T. Alamäe,et al.  Levansucrases of a Pseudomonas syringae pathovar as catalysts for the synthesis of potentially prebiotic oligo- and polysaccharides. , 2015, New biotechnology.

[5]  F. Plou,et al.  Levan versus fructooligosaccharide synthesis using the levansucrase from Zymomonas mobilis: effect of reaction conditions , 2015 .

[6]  L. Caputi,et al.  The crystal structure of Erwinia amylovora levansucrase provides a snapshot of the products of sucrose hydrolysis trapped into the active site. , 2015, Journal of structural biology.

[7]  D. Sheu,et al.  Antineoplastic effect of a novel chemopreventive agent, neokestose, on the Caco-2 cell line via inhibition of expression of nuclear factor-κB and cyclooxygenase-2. , 2015, Molecular medicine reports.

[8]  Michael J E Sternberg,et al.  The Phyre2 web portal for protein modeling, prediction and analysis , 2015, Nature Protocols.

[9]  F. Plou,et al.  Heterologous overproduction of β-fructofuranosidase from yeast Xanthophyllomyces dendrorhous, an enzyme producing prebiotic sugars , 2015, Applied Microbiology and Biotechnology.

[10]  Lili Lu,et al.  A recombinant levansucrase from Bacillus licheniformis 8-37-0-1 catalyzes versatile transfructosylation reactions , 2014 .

[11]  Pedro M. Coutinho,et al.  The carbohydrate-active enzymes database (CAZy) in 2013 , 2013, Nucleic Acids Res..

[12]  J. Mah,et al.  Antipathogenic activity and preservative effect of levan (β-2,6-fructan), a multifunctional polysaccharide , 2014 .

[13]  L. Caputi,et al.  Biomolecular characterization of the levansucrase of Erwinia amylovora, a promising biocatalyst for the synthesis of fructooligosaccharides. , 2013, Journal of agricultural and food chemistry.

[14]  H. Belghith,et al.  Partial purification of a Bacillus licheniformis levansucrase producing levan with antitumor activity. , 2012, International journal of biological macromolecules.

[15]  A. Gamal-Eldeen,et al.  Antitumor and antioxidant activities of levan and its derivative from the isolate Bacillus subtilis NRC1aza. , 2012, Carbohydrate polymers.

[16]  T. Alamäe,et al.  Levansucrases from Pseudomonas syringae pv. tomato and P. chlororaphis subsp. aurantiaca: substrate specificity, polymerizing properties and usage of different acceptors for fructosylation. , 2011, Journal of biotechnology.

[17]  D. Jahn,et al.  Polysaccharide Synthesis of the Levansucrase SacB from Bacillus megaterium Is Controlled by Distinct Surface Motifs* , 2011, The Journal of Biological Chemistry.

[18]  Juan Lu,et al.  Isolation, structural characterization and immunological activity of an exopolysaccharide produced by Bacillus licheniformis 8-37-0-1. , 2010, Bioresource technology.

[19]  M. Sabater-Molina,et al.  Dietary fructooligosaccharides and potential benefits on health , 2009, Journal of Physiology and Biochemistry.

[20]  E. Rudiño-Piñera,et al.  Selected mutations in Bacillus subtilis levansucrase semi-conserved regions affecting its biochemical properties. , 2008, Protein engineering, design & selection : PEDS.

[21]  G. Visser,et al.  Glycosyltransferase-catalyzed synthesis of bioactive oligosaccharides. , 2008, Biotechnology advances.

[22]  R. Vogel,et al.  Genetic and functional characterization of Lactobacillus panis levansucrase , 2008, Archives of Microbiology.

[23]  K. Fütterer,et al.  Donor substrate recognition in the raffinose-bound E342A mutant of fructosyltransferase Bacillus subtilis levansucrase , 2008, BMC Structural Biology.

[24]  D. Jahn,et al.  Insights into polymer versus oligosaccharide synthesis: mutagenesis and mechanistic studies of a novel levansucrase from Bacillus megaterium. , 2007, The Biochemical journal.

[25]  L. Dijkhuizen,et al.  The levansucrase and inulosucrase enzymes of Lactobacillus reuteri 121 catalyse processive and non-processive transglycosylation reactions. , 2006, Microbiology.

[26]  C. Shieh,et al.  selective production and characterization of levan by Bacillus subtilis (Natto) Takahashi. , 2005, Journal of agricultural and food chemistry.

[27]  T. Pons,et al.  Crystal structure of levansucrase from the Gram-negative bacterium Gluconacetobacter diazotrophicus. , 2005, The Biochemical journal.

[28]  R. Vogel,et al.  Molecular and functional characterization of a levansucrase from the sourdough isolate Lactobacillus sanfranciscensis TMW 1.392 , 2005, Applied Microbiology and Biotechnology.

[29]  K. Fütterer,et al.  Structural framework of fructosyl transfer in Bacillus subtilis levansucrase , 2003, Nature Structural Biology.

[30]  Ji Young Yang,et al.  Enzymatic synthesis of fructosyl oligosaccharides by levansucrase from Microbacterium laevaniformans ATCC 15953 , 2003 .

[31]  L. Dijkhuizen,et al.  Kinetic properties of an inulosucrase from Lactobacillus reuteri 121 , 2003, FEBS letters.

[32]  U. Viesturs,et al.  Fructooligosaccharide and levan producing activity of Zymomonas mobilis extracellular levansucrase , 2002 .

[33]  G. Gibson,et al.  The effects of the novel bifidogenic trisaccharide, neokestose, on the human colonic microbiota , 2002 .

[34]  K. Okamoto,et al.  Identification of functionally important amino acid residues in Zymomonas mobilis levansucrase. , 2002, Journal of biochemistry.

[35]  L. Hernández,et al.  Fructo-oligosaccharides production by the Gluconacetobacter diazotrophicus levansucrase expressed in the methylotrophic yeast Pichia pastoris. , 2001, Enzyme and microbial technology.

[36]  W. Hartmeier,et al.  Metabolization of beta-(2,6)-linked fructose-oligosaccharides by different bifidobacteria. , 2000, FEMS microbiology letters.

[37]  R. Chambert,et al.  Polymerase and hydrolase activities of Bacillus subtilis levansucrase can be separately modulated by site-directed mutagenesis. , 1991, The Biochemical journal.

[38]  Toshio Tanaka,et al.  The mode of synthesis of levan by Bacillus subtilis levansucrase. , 1985 .

[39]  S. Oi,et al.  The molecular structure of low and high molecular weight levans synthesized by levansucrase. , 1980, Journal of biochemistry.

[40]  M. M. Bradford A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.