Bioactivities of extracts from Eugenia uniflora L. branches were investigated using in vitro and vivo tests. Dried and powdered from E. uniflora branches were exhaustivel y extracted with hexane, ethyl acetate and ethanol by static maceration obtaining the hexane (HE), ethyl acetate (EAE) and ethanol (EE) extracts. Phytochemical scr eening and total phenolic and flavonoid contents were determin ed. The antioxidant activity was assessed by 2,2-di phenyl-1pycrilhydrazin (DPPH) and iron reducing power tests . For evaluate the antinociceptive activity, acetic acid-induced writhing, formalin and hot plate assays were perfor med, while the anti-inflammatory activity by paw ed ema method. Flavonoids, tannins, coumarins, terpenes and sterol s were revealed in the extracts. HE, EAE and EE pre sented expressive levels of total phenolic and flavonoids and a promising antioxidant effect. Doses of HE, EA E or EE (50, 100 or 200 mg/kg) decreased the number of writhes a nd the phases of paw licking time. The analgesic ef fect on central level was confirmed by the increase in reac tion time on hot plate. EAE and EE (200 and 400 mg/ kg) and HE (400 mg/kg) showed a reduction of paw edema after 4 h of carrageenan application. Therefore, E. uniflo ra presented important biological properties that may be associated with the chemical constituents and co rrob rate the medicinal uses of this species.