High resolution melting curve analysis technology and application

High-resolution melting curve(HRM) is a new genetic analysis technology based on single nucleotide polymorphism in the formation of different forms of melting curves.It has a very high sensitivity,which can detect single nucleotide differences.Meanwhile,it has a great deal of other advantages such as low cost,high throughput,rapid,accuracy and not subject to the limitations of testing sites which realizes closed tube operation.HRM analysis technology is playing an important role in the fields of mutation scanning,single nucleotidepolymorphism analysis,methylation studies,genotyping,sequence matching and other aspects.The development of this method will surely have a far-reaching impact on the study of human genes.