Modulation of Connexin43 Expression:

Modulation of Cx43 Expression. Introduction: Gap junctions connect cardiac myocytes allowing propagation of action potentials. They contain intercellular channels formed by multiple different connexin proteins. The arrangement and type of gap junctions and the types, function, and interaction of connexin proteins determine intercellular resistance and can thereby influence conduction velocity and the potential for reentrant arrhythmias. Our goal was to develop genetically manipulable models to test the effects of altering expression of a major cardiac connexin (connexin43) on intercellular coupling and expression of other connexin proteins.

[1]  J. Saffitz,et al.  Expression of Multiple Gap Junction Proteins in Human Fetal and Infant Hearts , 1994, Pediatric Research.

[2]  J. Burt,et al.  Selective block of gap junction channel expression with connexin-specific antisense oligodeoxynucleotides. , 1994, The American journal of physiology.

[3]  J. Saffitz,et al.  Distinct gap junction protein phenotypes in cardiac tissues with disparate conduction properties. , 1994, Journal of the American College of Cardiology.

[4]  M. Spach.  Changes in the topology of gap junctions as an adaptive structural response of the myocardium. , 1994, Circulation.

[5]  J. Saffitz,et al.  Molecular cloning of two human cardiac gap junction proteins, connexin40 and connexin45. , 1994, Journal of molecular and cellular cardiology.

[6]  P. Brink,et al.  Connexin37 forms high conductance gap junction channels with subconductance state activity and selective dye and ionic permeabilities. , 1994, Biophysical journal.

[7]  A. Moreno,et al.  Human connexin43 gap junction channels. Regulation of unitary conductances by phosphorylation. , 1994, Circulation research.

[8]  P. S. Chen,et al.  The mechanism of termination of reentrant activity in ventricular fibrillation. , 1994, Circulation research.

[9]  T. Steinberg,et al.  Connexin43 and connexin45 form gap junctions with different molecular permeabilities in osteoblastic cells. , 1994, The EMBO journal.

[10]  C. Naus,et al.  Transfection of C6 glioma cells with connexin32: the effects of expression of a nonendogenous gap junction protein. , 1994, Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research.

[11]  D. Ganten,et al.  Gap junction protein connexin40 is preferentially expressed in vascular endothelium and conductive bundles of rat myocardium and is increased under hypertensive conditions. , 1993, Circulation research.

[12]  B. A. French,et al.  The Advent of Adenovirus Gene Therapy for Cardiovascular Disease , 1993, Circulation.

[13]  W H Lamers,et al.  Immunohistochemical delineation of the conduction system. I: The sinoatrial node. , 1993, Circulation research.

[14]  P. Poole‐Wilson,et al.  Reduced content of connexin43 gap junctions in ventricular myocardium from hypertrophied and ischemic human hearts. , 1993, Circulation.

[15]  J. Saffitz,et al.  Multiple connexins colocalize in canine ventricular myocyte gap junctions. , 1993, Circulation research.

[16]  R. Sager,et al.  Specificity of gap junction communication among human mammary cells and connexin transfectants in culture , 1993, The Journal of cell biology.

[17]  P. Corr,et al.  Inhibition of gap junctional conductance by long-chain acylcarnitines and their preferential accumulation in junctional sarcolemma during hypoxia. , 1993, Circulation research.

[18]  R. Veenstra,et al.  Molecular cloning and functional expression of human connexin37, an endothelial cell gap junction protein. , 1993, The Journal of clinical investigation.

[19]  A. Samarel,et al.  Formation of fetal rat cardiac cell clones by retroviral transformation: retention of select myocyte characteristics. , 1993, Journal of molecular and cellular cardiology.

[20]  P. Brink,et al.  Chick connexin-56, a novel lens gap junction protein. Molecular cloning and functional expression. , 1993, The Journal of biological chemistry.

[21]  R. Veenstra,et al.  Multiple connexins confer distinct regulatory and conductance properties of gap junctions in developing heart. , 1992, Circulation research.

[22]  Li Shaowei,et al.  Analysis of myosin light chains in myocardium from Keshan disease patients , 1992 .

[23]  A. Samarel,et al.  Growth factor modulation of myocyte protein and DNA synthesis , 1992 .

[24]  E. Hertzberg,et al.  Gap junction distribution is altered between cardiac myocytes infected with Trypanosoma cruzi. , 1992, Circulation research.

[25]  C. Kempf,et al.  Electrical coupling between cells of the insect Aedes albopictus. , 1992, The Journal of physiology.

[26]  J. Saffitz,et al.  Cardiac myocytes express multiple gap junction proteins. , 1992, Circulation research.

[27]  M. Bennett,et al.  Gap junctions formed by connexins 26 and 32 alone and in combination are differently affected by applied voltage. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[28]  L. Leinwand,et al.  Functional analysis of human cardiac gap junction channel mutants. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[29]  J E Saffitz,et al.  Remodeling of ventricular conduction pathways in healed canine infarct border zones. , 1991, The Journal of clinical investigation.

[30]  C. Naus,et al.  Transfection of C6 glioma cells with connexin 43 cDNA: analysis of expression, intercellular coupling, and cell proliferation. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[31]  A. Wilde,et al.  Ventricular tachycardia in the infarcted, Langendorff-perfused human heart: role of the arrangement of surviving cardiac fibers. , 1990, Journal of the American College of Cardiology.

[32]  J E Saffitz,et al.  Alterations of intercellular junctions induced by hypoxia in canine myocardium. , 1990, The American journal of physiology.

[33]  D. Spray,et al.  Expression of gap junction channels in communication-incompetent cells after stable transfection with cDNA encoding connexin 32. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[34]  R. Erickson,et al.  Abnormal development and dye coupling produced by antisense RNA to gap junction protein in mouse preimplantation embryos. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[35]  D. Paul,et al.  Antisera directed against connexin43 peptides react with a 43-kD protein localized to gap junctions in myocardium and other tissues , 1989, The Journal of cell biology.

[36]  E. Unanue,et al.  Expression of membrane interleukin 1 by fibroblasts transfected with murine pro-interleukin 1 alpha cDNA. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[37]  D C Spray,et al.  Inotropic agents modulate gap junctional conductance between cardiac myocytes. , 1988, The American journal of physiology.

[38]  J M de Bakker,et al.  Reentry as a cause of ventricular tachycardia in patients with chronic ischemic heart disease: electrophysiologic and anatomic correlation. , 1988, Circulation.

[39]  D. Paul,et al.  Connexin43: a protein from rat heart homologous to a gap junction protein from liver , 1987, The Journal of cell biology.

[40]  P. Chomczyński,et al.  Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. , 1987, Analytical biochemistry.

[41]  A H Harken,et al.  Recurrent sustained ventricular tachycardia: structure and ultrastructure of subendocardial regions in which tachycardia originates. , 1983, Circulation.

[42]  M. M. Bradford.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.

[43]  M. Bennett,et al.  Erratum: Gap junctions formed by connexins 26 and 32 alone and in combination are differently affected by applied voltage (Proc. Natl. Acad. Sci. USA (October 1, 1991) 88 (8410-8414)) , 1992 .

[44]  W. D. De Mello,et al.  Influence of cyclic nucleotides on junctional permeability in atrial muscle. , 1987, Journal of Molecular and Cellular Cardiology.