Micro-optical fiber probe for use in an intravascular Raman endoscope.

We believe that we have developed the narrowest optical-fiber Raman probe ever reported, 600 microm in total diameter, that can be inserted into coronary arteries. The selection of suitable optical fibers, filters, and a processing method is discussed. Custom-made filters attached to the front end of a probe eliminate the background Raman signals of the optical fiber itself. The experimental evaluation of various optical fibers is carried out for the selection of suitable fibers. Measurement of the Raman spectra of an atherosclerotic lesion of a rabbit artery in vitro demonstrates the excellent performance of the micro-Raman probe.

[1]  E. Halpern,et al.  Quantification of Macrophage Content in Atherosclerotic Plaques by Optical Coherence Tomography , 2003, Circulation.

[2]  K. P. J. Williams,et al.  Remote sampling using a fibre-optic probe in fourier transform Raman spectroscopy , 1990 .

[3]  Katsuo Aizawa,et al.  Change in chemical composition of lipids accumulated in atheromas of rabbits following photodynamic therapy , 1997, Lasers in surgery and medicine.

[4]  Hideo Tashiro,et al.  Fluorescence Backgroundless Ti: Sapphire Laser Using Acousto-Optical Tunable Filter for Raman Spectroscopic Measurements , 2002 .

[5]  S. Wada,et al.  Biomedical applications of a new portable Raman imaging probe , 2001 .

[6]  Michael S Feld,et al.  Optical fiber probe for biomedical Raman spectroscopy. , 2004, Applied optics.

[7]  B. Wilson,et al.  Development of an In Vivo Raman Spectroscopic System for Diagnostic Applications , 1997 .

[8]  R. Richards-Kortum,et al.  Near-Infrared Raman Spectroscopy for in vivo Detection of Cervical Precancers , 2001, Photochemistry and photobiology.

[9]  H. Crispin,et al.  Intravascular observation and surgery using the flexible fibrescope. , 1973, Lancet.

[10]  A F van der Steen,et al.  Intravascular ultrasound combined with Raman spectroscopy to localize and quantify cholesterol and calcium salts in atherosclerotic coronary arteries. , 2000, Arteriosclerosis, thrombosis, and vascular biology.

[11]  N. Ramanujam,et al.  Development of a Fiber Optic Probe to Measure NIR Raman Spectra of Cervical Tissue In Vivo , 1998, Photochemistry and photobiology.

[12]  I. R. Lewis,et al.  Raman Spectrometry with Fiber-Optic Sampling , 1996 .

[13]  S. Angel,et al.  Comparative Study of Some Fiber-Optic Remote Raman Probe Designs. Part I: Model for Liquids and Transparent Solids , 1996 .

[14]  Hideo Tashiro,et al.  Noninvasive Measurement of Oxygenation of Hemoglobin by Direct Transmission of Near-Infrared Energy (700–1000 nm) from an Electronically Tuned Ti: Sapphire Laser Driven by a Dual Radio-Frequency Driving Method , 2000 .

[15]  H Tashiro,et al.  Excitation wavelength-dependent changes in Raman spectra of whole blood and hemoglobin: comparison of the spectra with 514.5-, 720-, and 1064-nm excitation. , 2001, Journal of biomedical optics.

[16]  Lawrence R. Procell,et al.  Nonintrusive Analysis of Chemical Agent Identification Sets Using a Portable Fiber-Optic Raman Spectrometer , 1999 .

[17]  R. Dasari,et al.  Prospects for in vivo Raman spectroscopy , 2000 .

[18]  J. Rolland,et al.  Letter: diogenes syndrome. , 1975, Lancet.

[19]  Brian C. Wilson,et al.  In vivo Near-infrared Raman Spectroscopy: Demonstration of Feasibility During Clinical Gastrointestinal Endoscopy¶ , 2000, Photochemistry and photobiology.

[20]  Hideo Tashiro,et al.  Qualitative analysis of trace Raman bands of non-resonant species in near-infrared excited pre-resonance Raman spectra of hemoglobin by partial least square regression-Raman excitation profile method , 2004 .

[21]  S. Achenbach,et al.  Detection of Calcified and Noncalcified Coronary Atherosclerotic Plaque by Contrast-Enhanced, Submillimeter Multidetector Spiral Computed Tomography: A Segment-Based Comparison With Intravascular Ultrasound , 2003, Circulation.

[22]  Michael L. Wach,et al.  In vivo determination of the molecular composition of artery wall by intravascular Raman spectroscopy. , 2000, Analytical chemistry.

[23]  M. Takano,et al.  Changes in coronary plaque color and morphology by lipid-lowering therapy with atorvastatin: serial evaluation by coronary angioscopy. , 2003, Journal of the American College of Cardiology.

[24]  R. Rava,et al.  Quantitative histochemical analysis of human artery using Raman spectroscopy. , 1992, Journal of photochemistry and photobiology. B, Biology.

[25]  Brian C. Wilson,et al.  Study of Fiber-Optic Probes for in vivo Medical Raman Spectroscopy , 1999 .

[26]  C J de Lima,et al.  Development of catheters with low fiber background signals for Raman spectroscopic diagnosis applications. , 2000, Artificial organs.

[27]  J. Ma,et al.  Fiber Raman background study and its application in setting up optical fiber Raman probes. , 1996, Applied optics.