Characterization of a Glycoside Hydrolase Family 27 α-Galactosidase from Pontibacter Reveals Its Novel Salt-Protease Tolerance and Transglycosylation Activity.

α-Galactosidases are of great interest in various applications. A glycoside hydrolase family 27 α-galactosidase was cloned from Pontibacter sp. harbored in a saline soil and expressed in Escherichia coli. The purified recombinant enzyme (rAgaAHJ8) was little or not affected by 3.5-30.0% (w/v) NaCl, 10.0-100.0 mM Pb(CH3COO)2, 10.0-60.0 mM ZnSO4, or 8.3-100.0 mg mL(-1) trypsin and by most metal ions and chemical reagents at 1.0 and 10.0 mM concentrations. The degree of synergy on enzymatic degradation of locust bean gum and guar gum by an endomannanase and rAgaAHJ8 was 1.22-1.54. In the presence of trypsin, the amount of reducing sugars released from soybean milk treated by rAgaAHJ8 was approximately 3.8-fold compared with that treated by a commercial α-galactosidase. rAgaAHJ8 showed transglycosylation activity when using sucrose, raffinose, and 3-methyl-1-butanol as the acceptors. Furthermore, potential factors for salt adaptation of the enzyme were presumed.

[1]  Junjun Li,et al.  Characterization of two glycoside hydrolase family 36 α-galactosidases: novel transglycosylation activity, lead-zinc tolerance, alkaline and multiple pH optima, and low-temperature activity. , 2016, Food chemistry.

[2]  P. Shi,et al.  Biochemical characterization of a novel thermophilic α-galactosidase from Talaromyces leycettanus JCM12802 with significant transglycosylation activity. , 2016, Journal of bioscience and bioengineering.

[3]  Junpei Zhou,et al.  A novel surfactant-, NaCl-, and protease-tolerant β-mannanase from Bacillus sp. HJ14 , 2016, Folia Microbiologica.

[4]  Shane A. Seabrook,et al.  Rational engineering of a mesohalophilic carbonic anhydrase to an extreme halotolerant biocatalyst , 2015, Nature Communications.

[5]  Q. Gao,et al.  Unraveling adaptation of Pontibacter korlensis to radiation and infertility in desert through complete genome and comparative transcriptomic analysis , 2015, Scientific Reports.

[6]  M. Kurakake,et al.  Synthesis of galactosyl glycerol from guar gum by transglycosylation of α-galactosidase from Aspergillus sp. MK14. , 2015, Food chemistry.

[7]  S. Packman,et al.  Ten-year outcome of enzyme replacement therapy with agalsidase beta in patients with Fabry disease , 2015, Journal of Medical Genetics.

[8]  Junpei Zhou,et al.  Molecular and Biochemical Characterization of a Novel Multidomain Xylanase from Arthrobacter sp. GN16 Isolated from the Feces of Grus nigricollis , 2014, Applied Biochemistry and Biotechnology.

[9]  P. Shi,et al.  A New α-Galactosidase from Thermoacidophilic Alicyclobacillus sp. A4 with Wide Acceptor Specificity for Transglycosylation , 2014, Applied Biochemistry and Biotechnology.

[10]  C. Sasikala,et al.  Pontibacter ruber sp. nov. and Pontibacter deserti sp. nov., isolated from the desert. , 2014, International journal of systematic and evolutionary microbiology.

[11]  Z. Shao,et al.  Cloning, expression and characterization of a novel cold-active and halophilic xylanase from Zunongwangia profunda , 2014, Extremophiles.

[12]  Ziduo Liu,et al.  A novel cold-active and salt-tolerant α-amylase from marine bacterium Zunongwangia profunda: molecular cloning, heterologous expression and biochemical characterization , 2013, Extremophiles.

[13]  Jian‐He Xu,et al.  Heterologous expression and characterization of a novel thermo-halotolerant endoglucanase Cel5H from Dictyoglomus thermophilum. , 2013, Bioresource technology.

[14]  M. Fons,et al.  Functional Analysis of Family GH36 α-Galactosidases from Ruminococcus gnavus E1: Insights into the Metabolism of a Plant Oligosaccharide by a Human Gut Symbiont , 2012, Applied and Environmental Microbiology.

[15]  Junpei Zhou,et al.  A novel xylanase with tolerance to ethanol, salt, protease, SDS, heat, and alkali from actinomycete Lechevalieria sp. HJ3 , 2012, Journal of Industrial Microbiology & Biotechnology.

[16]  M. Kurakake,et al.  Enzymatic properties and transglycosylation of α-galactosidase from Penicillium oxalicum SO , 2011 .

[17]  K. Meng,et al.  Properties of a novel α-galactosidase from Streptomyces sp. S27 and its potential for soybean processing , 2010 .

[18]  P. Shi,et al.  An alpha-galactosidase from an acidophilic Bispora sp. MEY-1 strain acts synergistically with beta-mannanase. , 2010, Bioresource technology.

[19]  B. Svensson,et al.  Aspergillus nidulansα‐galactosidase of glycoside hydrolase family 36 catalyses the formation of α‐galacto‐oligosaccharides by transglycosylation , 2010, The FEBS journal.

[20]  K. Meng,et al.  A new α-galactosidase from symbiotic Flavobacterium sp. TN17 reveals four residues essential for α-galactosidase activity of gastrointestinal bacteria , 2010, Applied Microbiology and Biotechnology.

[21]  K. Shivam,et al.  Purification and characterization of a thermostable α-galactosidase with transglycosylation activity from Aspergillus parasiticus MTCC-2796 , 2010 .

[22]  Z. Ogel,et al.  Cloning and heterologous expression of the extracellular alpha-galactosidase from Aspergillus fumigatus in Aspergillus sojae under the control of gpdA promoter , 2010 .

[23]  G. Gibson,et al.  A novel α-galactosidase from Βifidobacterium bifidum with transgalactosylating properties: gene molecular cloning and heterologous expression , 2009, Applied Microbiology and Biotechnology.

[24]  V. Křen,et al.  α-Galactosidases and their applications in biotransformations , 2009 .

[25]  S. Kuzmanova,et al.  Omjer reakcija transgalaktozilacije i hidrolize različitih β-galaktozidaza što kataliziraju sintezu alkil-β-galaktozida u monofaznim alkoholnim medijima , 2008 .

[26]  H. Kumagai,et al.  Cloning and characterization of a novel alpha-galactosidase from Bifidobacterium breve 203 capable of synthesizing Gal-alpha-1,4 linkage. , 2008, FEMS microbiology letters.

[27]  C. Dutta,et al.  Molecular signature of hypersaline adaptation: insights from genome and proteome composition of halophilic prokaryotes , 2008, Genome Biology.

[28]  K. Meng,et al.  Purification and characterization of a novel protease-resistant α-galactosidase from Rhizopus sp. F78 ACCC 30795 , 2007 .

[29]  B. Patel,et al.  Crystal structure of AmyA lacks acidic surface and provide insights into protein stability at poly‐extreme condition , 2006, FEBS letters.

[30]  J. Sussman,et al.  Three-dimensional structure of a halotolerant algal carbonic anhydrase predicts halotolerance of a mammalian homolog. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[31]  B. Luh,et al.  Industrial production of soy sauce , 1995, Journal of Industrial Microbiology.

[32]  V. E. Luebke,et al.  Purification, cloning, and properties of α-galactosidase from Saccharopolyspora erythraea and its use as a reporter system , 2005, Applied Microbiology and Biotechnology.

[33]  A. N. Savel'ev,et al.  Crystal structure of alpha-galactosidase from Trichoderma reesei and its complex with galactose: implications for catalytic mechanism. , 2004, Journal of molecular biology.

[34]  P. Punt,et al.  Cloning and expression of a member of the Aspergillus niger gene family encoding α-galactosidase , 1992, Molecular and General Genetics MGG.

[35]  S. Ghazi,et al.  Improvement of the nutritive value of soybean meal by protease and a-galactosidase treatment in broiler cockerels and broiler chicks , 2003, British poultry science.

[36]  David S. Wishart,et al.  VADAR: a web server for quantitative evaluation of protein structure quality , 2003, Nucleic Acids Res..

[37]  Z. Fujimoto,et al.  Crystal Structure of Rice α-Galactosidase Complexed with D-Galactose* , 2003, Journal of Biological Chemistry.

[38]  A. N. Savel'ev,et al.  Enzymatic properties of α-galactosidase from Trichoderma reesei in the hydrolysis of galactooligosaccharides , 2002 .

[39]  K. Sakka,et al.  α-Galactosidase Aga27A, an Enzymatic Component of the Clostridium josui Cellulosome , 2002, Journal of bacteriology.

[40]  F. Schinner,et al.  Potential of halotolerant and halophilic microorganisms for biotechnology , 2001, Extremophiles.

[41]  H. Gilbert,et al.  alpha-Galactosidase A from Pseudomonas fluorescens subsp. cellulosa: cloning, high level expression and its role in galactomannan hydrolysis. , 2000, FEMS microbiology letters.

[42]  H. Gilbert,et al.  A comparison of enzyme-aided bleaching of softwood paper pulp using combinations of xylanase, mannanase and α-galactosidase , 2000, Applied Microbiology and Biotechnology.

[43]  D. Madern,et al.  Halophilic adaptation of enzymes , 2000, Extremophiles.

[44]  D. Madern,et al.  Halophilic adaptation: novel solvent protein interactions observed in the 2.9 and 2.6 A resolution structures of the wild type and a mutant of malate dehydrogenase from Haloarcula marismortui. , 2000, Biochemistry.

[45]  A. N. Savel'ev,et al.  Transglycosylation activity of alpha-D-galactosidase from Trichoderma reesei. An investigation of the active site. , 1997, Carbohydrate research.

[46]  K Schulten,et al.  VMD: visual molecular dynamics. , 1996, Journal of molecular graphics.

[47]  M. Goto,et al.  Transgalactosylation Catalyzed by α-Galactosidase from Candida guilliermondii H-404 , 1995 .

[48]  A. Zhu,et al.  Cloning and functional expression of a cDNA encoding coffee bean α-galactosidase , 1994 .

[49]  A. Böck,et al.  Production of thermostable, recombinant α-galactosidase suitable for raffinose elimination from sugar beet syrup , 1988 .