Biofabrication of Fe3O4 Nanoparticles from Spirogyra hyalina and Ajuga bracteosa and Their Antibacterial Applications

Iron oxide nanoparticles (NPs) have attracted substantial interest due to their superparamagnetic features, biocompatibility, and nontoxicity. The latest progress in the biological production of Fe3O4 NPs by green methods has improved their quality and biological applications significantly. In this study, the fabrication of iron oxide NPs from Spirogyra hyalina and Ajuga bracteosa was conducted via an easy, environmentally friendly, and cost-effective process. The fabricated Fe3O4 NPs were characterized using various analytical methods to study their unique properties. UV-Vis absorption peaks were observed in algal and plant-based Fe3O4 NPs at 289 nm and 306 nm, respectively. Fourier transform infrared (FTIR) spectroscopy analyzed diverse bioactive phytochemicals present in algal and plant extracts that functioned as stabilizing and capping agents in the fabrication of algal and plant-based Fe3O4 NPs. X-ray diffraction of NPs revealed the crystalline nature of both biofabricated Fe3O4 NPs and their small size. Scanning electron microscopy (SEM) revealed that algae and plant-based Fe3O4 NPs are spherical and rod-shaped, averaging 52 nm and 75 nm in size. Energy dispersive X-ray spectroscopy showed that the green-synthesized Fe3O4 NPs require a high mass percentage of iron and oxygen to ensure their synthesis. The fabricated plant-based Fe3O4 NPs exhibited stronger antioxidant properties than algal-based Fe3O4 NPs. The algal-based NPs showed efficient antibacterial potential against E. coli, while the plant-based Fe3O4 NPs displayed a higher zone of inhibition against S. aureus. Moreover, plant-based Fe3O4 NPs exhibited superior scavenging and antibacterial potential compared to the algal-based Fe3O4 NPs. This might be due to the greater number of phytochemicals in plants that surround the NPs during their green fabrication. Hence, the capping of bioactive agents over iron oxide NPs improves antibacterial applications.

[1]  Jingmao Zhao,et al.  Benzothiazole derivatives-based supramolecular assemblies as efficient corrosion inhibitors for copper in artificial seawater: Formation, interfacial release and protective mechanisms , 2023, Corrosion Science.

[2]  Baomin Fan,et al.  Ambient-stable polyethyleneimine functionalized Ti3C2T nanohybrid corrosion inhibitor for copper in alkaline electrolyte , 2023, Materials Letters.

[3]  F. Zhao,et al.  Designing the anti-biofouling surface of an ultrafiltration membrane with a novel zwitterionic poly(aryl ether oxadiazole) containing benzimidazole , 2022, Applied Surface Science.

[4]  Ying-Ming Pan,et al.  Electrochemically promoted N-heterocyclic carbene polymer-catalyzed cycloaddition of aldehyde with isocyanide acetate , 2022, Science China Chemistry.

[5]  S. Salem,et al.  Environmental Impacts of Ecofriendly Iron Oxide Nanoparticles on Dyes Removal and Antibacterial Activity , 2022, Applied Biochemistry and Biotechnology.

[6]  Henry Fenekansi Kiwumulo,et al.  Green synthesis and characterization of iron-oxide nanoparticles using Moringa oleifera: a potential protocol for use in low and middle income countries , 2022, BMC research notes.

[7]  N. Zhu,et al.  Near-infrared responsive Z-scheme heterojunction with strong stability and ultra-high quantum efficiency constructed by lanthanide-doped glass , 2022, Applied Catalysis B: Environmental.

[8]  Jiawei Lu,et al.  Synthesis and properties of Poly(vinyl alcohol) hydrogels with high strength and toughness , 2022, Polymer Testing.

[9]  Man Liu,et al.  Dysfunction of FadA-cAMP signalling decreases Aspergillus flavus resistance to antimicrobial natural preservative Perillaldehyde and AFB1 biosynthesis. , 2022, Environmental microbiology.

[10]  María Julia Martín,et al.  Biomedical Applications of Iron Oxide Nanoparticles: Current Insights Progress and Perspectives , 2022, Pharmaceutics.

[11]  Xiaokun Fan,et al.  A 4arm-PEG macromolecule crosslinked chitosan hydrogels as antibacterial wound dressing. , 2021, Carbohydrate polymers.

[12]  B. Abbasi,et al.  Plant-Based Green Synthesis of Nanoparticles: Production, Characterization and Applications , 2021, Biomolecules.

[13]  M. Qadir,et al.  Biologically synthesized iron nanoparticles (FeNPs) from Phoenix dactylifera have anti-bacterial activities , 2021, Scientific Reports.

[14]  Sathish Dyawanapelly,et al.  Bionanofactories for Green Synthesis of Silver Nanoparticles: Toward Antimicrobial Applications , 2021, International journal of molecular sciences.

[15]  B. Mirza,et al.  Antibacterial, Antihemolytic, Cytotoxic, Anticancer, and Antileishmanial Effects of Ajuga bracteosa Transgenic Plants , 2021, Plants.

[16]  S. Sasmal,et al.  A Review of Green Synthesis of Metal Nanoparticles Using Algae , 2021, Frontiers in Microbiology.

[17]  Shengyan Pu,et al.  Enhanced heterogeneous activation of peroxymonosulfate by boosting internal electron transfer in a bimetallic Fe3O4-MnO2 nanocomposite , 2021, Chinese Chemical Letters.

[18]  Taeho Lee,et al.  Nano zero-valent iron improves anammox activity by promoting the activity of quorum sensing system. , 2021, Water research.

[19]  X. Lou,et al.  Accelerate gas diffusion-weighted MRI for lung morphometry with deep learning , 2021, European Radiology.

[20]  A. Lisitsyn,et al.  Do Iron Oxide Nanoparticles Have Significant Antibacterial Properties? , 2021, Antibiotics.

[21]  Á. González-Delgado,et al.  Environmental Sustainability Evaluation of Iron Oxide Nanoparticles Synthesized via Green Synthesis and the Coprecipitation Method: A Comparative Life Cycle Assessment Study , 2021, ACS omega.

[22]  K. Saravanakumar,et al.  Antimicrobial and Wound Healing Properties of FeO Fabricated Chitosan/PVA Nanocomposite Sponge , 2021, Antibiotics.

[23]  M. El-sheekh,et al.  Green synthesis, characterization applications of iron oxide nanoparticles for antialgal and wastewater bioremediation using three brown algae , 2021, International journal of phytoremediation.

[24]  A. Arumugam,et al.  One-pot green synthesis of iron oxide nanoparticles from Bauhinia tomentosa: Characterization and application towards synthesis of 1, 3 diolein , 2021, Scientific Reports.

[25]  Wei Zhong,et al.  Multifunctional Ag(I)/CAAA-Amidphos Complex-Catalyzed Asymmetric [3 + 2] Cycloaddition of α-Substituted Acrylamides , 2021 .

[26]  Jiachao Yao,et al.  Improvement of Alcaligenes sp.TB performance by Fe-Pd/multi-walled carbon nanotubes: Enriched denitrification pathways and accelerated electron transport. , 2021, Bioresource technology.

[27]  M. Ashaduzzaman,et al.  Green synthesis of iron oxide nanoparticle using Carica papaya leaf extract: application for photocatalytic degradation of remazol yellow RR dye and antibacterial activity , 2020, Heliyon.

[28]  Alap Ali Zahid,et al.  Phytochemical-assisted biosynthesis of silver nanoparticles from Ajuga bracteosa for biomedical applications , 2020, Materials Research Express.

[29]  Alibakhsh Kasaeian,et al.  Role of Nanofluids in Drug Delivery and Biomedical Technology: Methods and Applications , 2020, Nanotechnology, science and applications.

[30]  E. M. Castanheira,et al.  Shape Anisotropic Iron Oxide-Based Magnetic Nanoparticles: Synthesis and Biomedical Applications , 2020, International journal of molecular sciences.

[31]  S. G. Hassan,et al.  Green synthesis of iron oxide nanorods using Withania coagulans extract improved photocatalytic degradation and antimicrobial activity. , 2020, Journal of photochemistry and photobiology. B, Biology.

[32]  M. Iqbal,et al.  Green synthesis of iron oxide nanoparticles using pomegranate seeds extract and photocatalytic activity evaluation for the degradation of textile dye , 2019, Journal of Materials Research and Technology.

[33]  Lakshmi Kalyani Ruddaraju,et al.  Antibacterial efficacy of green synthesized α-Fe2O3 nanoparticles using Sida cordifolia plant extract , 2019, Heliyon.

[34]  G. Benelli.  Green Synthesis of Nanomaterials , 2019, Nanomaterials.

[35]  F. Ezema,et al.  Biogenic synthesis of iron oxide nanorods using Moringa oleifera leaf extract for antibacterial applications , 2019, Applied Nanoscience.

[36]  M. Kawachi,et al.  Identification of 13 Spirogyra species (Zygnemataceae) by traits of sexual reproduction induced under laboratory culture conditions , 2019, Scientific Reports.

[37]  Bipin Chapagain,et al.  Himalayan honey loaded iron oxide nanoparticles: synthesis, characterization and study of antioxidant and antimicrobial activities , 2019, International journal of nanomedicine.

[38]  Aarti R. Deshmukh,et al.  Ultrasound Assisted Green Synthesis of Silver and Iron Oxide Nanoparticles Using Fenugreek Seed Extract and Their Enhanced Antibacterial and Antioxidant Activities , 2019, BioMed research international.

[39]  A. Pugazhendhi,et al.  Biosynthesis of iron oxide nanoparticles using leaf extract of Ruellia tuberosa: Antimicrobial properties and their applications in photocatalytic degradation. , 2019, Journal of photochemistry and photobiology. B, Biology.

[40]  A. Sazili,et al.  Rapid Green Synthesis and Characterization of Silver Nanoparticles Arbitrated by Curcumin in an Alkaline Medium , 2019, Molecules.

[41]  Y. Yıldız,et al.  Green synthesis and characterization of iron oxide nanoparticles using Ficus carica (common fig) dried fruit extract. , 2019, Journal of bioscience and bioengineering.

[42]  Seyed Mohammadali Dadfar,et al.  Iron oxide nanoparticles: Diagnostic, therapeutic and theranostic applications , 2019, Advanced drug delivery reviews.

[43]  V. Kumar,et al.  Green Synthesis and Characterization of Iron Oxide Nanoparticles Using Phyllanthus Niruri Extract , 2018 .

[44]  Alberto Carlos Botazzo Delbem,et al.  Iron Oxide Nanoparticles for Biomedical Applications: A Perspective on Synthesis, Drugs, Antimicrobial Activity, and Toxicity , 2018, Antibiotics.

[45]  Sanjay Singh,et al.  Recent advances and future prospects of iron oxide nanoparticles in biomedicine and diagnostics , 2018, 3 Biotech.

[46]  H. Naegeli,et al.  Critical review of the safety assessment of titanium dioxide additives in food , 2018, Journal of Nanobiotechnology.

[47]  Lazhen Shen,et al.  Fe3O4 Nanoparticles in Targeted Drug/Gene Delivery Systems , 2018, Materials.

[48]  A. Izadi,et al.  Evaluation of antifungal effect of iron-oxide nanoparticles against different Candida species , 2017 .

[49]  A. Putra.  Biofiltration Efficiency of Algae Spirogyra hyalina to Reduce Salinity of Sea Water , 2017 .

[50]  L. Shao,et al.  The antimicrobial activity of nanoparticles: present situation and prospects for the future , 2017, International journal of nanomedicine.

[51]  B. Mirza,et al.  Evaluation of Ajuga bracteosa for antioxidant, anti-inflammatory, analgesic, antidepressant and anticoagulant activities , 2016, BMC Complementary and Alternative Medicine.

[52]  V. Subha,et al.  Green synthesis of iron oxide nanoparticles from Mimosa pudica root extract , 2016 .

[53]  K. Shameli,et al.  Green Synthesis of Magnetite (Fe3O4) Nanoparticles Using Seaweed (Kappaphycus alvarezii) Extract , 2016, Nanoscale Research Letters.

[54]  Sutiman Bambang Sumitro,et al.  Bioethanol production from algae Spirogyra hyalina using Zymomonas mobilis , 2016 .

[55]  Y. R. Lee,et al.  Green fabrication of ferromagnetic Fe3O4 nanoparticles and their novel catalytic applications for the synthesis of biologically interesting benzoxazinone and benzthioxazinone derivatives , 2014 .

[56]  T. Balaji,et al.  Biogenic synthesis of Fe3O4 magnetic nanoparticles using plantain peel extract , 2013 .

[57]  J. Kumar,et al.  Removal of heavy metals by biosorption using freshwater alga Spirogyra hyalina. , 2012, Journal of environmental biology.

[58]  Jianguo Tian,et al.  Nonlinear optical and optical limiting properties of graphene oxide–Fe3O4 hybrid material , 2011 .

[59]  Yuhan Sun,et al.  Transformation of ferrihydrite in the presence or absence of trace Fe(II): The effect of preparation procedures of ferrihydrite , 2009 .