Accordance and concordance of PCR and NASBA followed by oligochromatography for the molecular diagnosis of Trypanosoma brucei and Leishmania

Objective  To evaluate the repeatability and reproducibility of four simplified molecular assays for the diagnosis of Trypanosoma brucei spp. or Leishmania ssp. in a multicentre ring trial with seven participating laboratories.

[1]  G. Schoone,et al.  Simplified molecular detection of Leishmania parasites in various clinical samples from patients with leishmaniasis , 2010, Parasites & Vectors.

[2]  G. Lubega,et al.  Nucleic Acid Sequence-Based Amplification with Oligochromatography for Detection of Trypanosoma brucei in Clinical Samples , 2009, Journal of Clinical Microbiology.

[3]  P. Herdewijn,et al.  A Simplified and Standardized Polymerase Chain Reaction Format for the Diagnosis of Leishmaniasis , 2008, The Journal of infectious diseases.

[4]  G. Lubega,et al.  Detection of Trypanosoma brucei parasites in blood samples using real-time nucleic acid sequence-based amplification. , 2008, Diagnostic microbiology and infectious disease.

[5]  M. Farenhorst,et al.  Comparison between Quantitative Nucleic Acid Sequence-Based Amplification, Real-Time Reverse Transcriptase PCR, and Real-Time PCR for Quantification of Leishmania Parasites , 2007, Journal of Clinical Microbiology.

[6]  D. Verloo,et al.  Validation of a PCR-Oligochromatography Test for Detection of Trypanozoon Parasites in a Multicenter Collaborative Trial , 2007, Journal of Clinical Microbiology.

[7]  P. Herdewijn,et al.  Molecular Dipstick Test for Diagnosis of Sleeping Sickness , 2006, Journal of Clinical Microbiology.

[8]  G. Schoone,et al.  Quantitative Nucleic Acid Sequence-Based Assay as a New Molecular Tool for Detection and Quantification of Leishmania Parasites in Skin Biopsy Samples , 2005, Journal of Clinical Microbiology.

[9]  N. Nagelkerke,et al.  Analysing collaborative trials for qualitative microbiological methods: accordance and concordance. , 2002, International journal of food microbiology.

[10]  Debashis Kushary,et al.  Bootstrap Methods and Their Application , 2000, Technometrics.

[11]  M. Salimans,et al.  Rapid and simple method for purification of nucleic acids , 1990, Journal of clinical microbiology.

[12]  H. van der Voet,et al.  Estimation of accordance and concordance in inter-laboratory trials of analytical methods with qualitative results. , 2004, International journal of food microbiology.