Study Potential of Indigenous Pseudomonas aeruginosa and Bacillus subtilis in Bioremediation of Diesel-Contaminated Water

Petroleum products which are used in a wide variety of industries as energy sources and raw materials have become a major concern in pollution of terrestrial and marine environments. The purpose of this study was to assess the potential of indigenous microbial isolates for degradation of diesel fuel. Two most proficient bacterial strains among five isolated strains from polluted soil of an industrial refinery were studied. The isolates then were identified as Pseudomonas aeruginosa and Bacillus subtilis using biochemical tests and 16S rRNA gene sequence analyses. P. aeruginosa showed higher biodegradation efficiency than B. subtilis in shaking flask containing diesel-contaminated water. P. aeruginosa and B. subtilis degraded about 87 and 75% of total hydrocarbons, respectively, in flasks containing 2% diesel and 98% water. The biodegradation efficiency of the isolates decreased as diesel contamination increased from 2 to 5%. The isolates showed significantly higher efficiency on degradation of short-chain hydrocarbons in 20 days, i.e., by using P. aeruginosa, removal efficiency of C10 hydrocarbons was near 90%, while about 69% of C20+ hydrocarbons and 47% of aromatic hydrocarbons were removed. Therefore, the isolates showed high capability in biodegradation of diesel contamination of the refinery.

[1]  Zheng Lu,et al.  Aerobic degradation of methyl tert-butyl ether by a Proteobacteria strain in a closed culture system. , 2007, Journal of environmental sciences.

[2]  S. Mukherji,et al.  Biodegradation rate of diesel range n-alkanes by bacterial cultures exiguobacterium aurantiacum and burkholderia cepacia , 2008 .

[3]  R. Surampalli,et al.  Control of petroleum-hydrocarbon contaminated groundwater by intrinsic and enhanced bioremediation. , 2010, Journal of environmental sciences.

[4]  J. Shao,et al.  Enhanced Biodegradation of Diesel Oil in Seawater Supplemented with Nutrients , 2006 .

[5]  W. Shieh,et al.  Biodegradation of dispersed diesel fuel under high salinity conditions , 2000 .

[6]  P. Ndegwa,et al.  Bioremediation of oil-contaminated soil using Candida catenulata and food waste. , 2008, Environmental pollution.

[7]  I. Head.  Bioremediation: towards a credible technology. , 1998, Microbiology.

[8]  G. Emtiazi,et al.  Biodegradation of Petroleum and Aromatic Hydrocarbons by Bacteria Isolated from Petroleum-Contaminated Soil , 2010 .

[9]  F. Schinner,et al.  Bioremediation (Natural Attenuation and Biostimulation) of Diesel-Oil-Contaminated Soil in an Alpine Glacier Skiing Area , 2001, Applied and Environmental Microbiology.

[10]  Sanket J. Joshi,et al.  Isolation and Characterization of Hydrocarbon Degrading Bacterial Isolate from Oil Contaminated Sites , 2013 .

[11]  V. Matei,et al.  Research on the Influence of Diesel Oil on the Bacterial Stems of Bacillus Subtilis and Pseudomonas Aeruginosa , 2011 .

[12]  B. Okeke,et al.  Comparative bioremediation of soils contaminated with diesel oil by natural attenuation, biostimulation and bioaugmentation. , 2005, Bioresource technology.

[13]  R. Kahawita,et al.  Modelling the fate of pollutant in overland flow , 2000 .

[14]  Cuiqing Ma,et al.  Degradation of n-alkanes and polycyclic aromatic hydrocarbons in petroleum by a newly isolated Pseudomonas aeruginosa DQ8. , 2011, Bioresource technology.

[15]  Y. Y. Hu,et al.  Investigation on bioremediation of oil-polluted wetland at Liaodong Bay in northeast China , 2006, Applied Microbiology and Biotechnology.

[16]  J. Aislabie,et al.  Bioremediation of hydrocarbon-contaminated polar soils , 2006, Extremophiles.

[17]  M. Hasanuzzaman,et al.  Verification of Degradation of n-Alkanes in Diesel Oil by Pseudomonas aeruginosa Strain WatG in Soil Microcosms , 2006, Current Microbiology.

[18]  Kerstin E. Scherr,et al.  Isolation and identification of biosurfactant producing and crude oil degrading Pseudomonas aeruginosa strains , 2012 .

[19]  S. Varjani,et al.  Biodegradation of petroleum hydrocarbons by oleophilic strain of Pseudomonas aeruginosa NCIM 5514. , 2016, Bioresource technology.

[20]  T. Jesionowski,et al.  Biodegradation of alkyl derivatives of aromatic hydrocarbons and cell surface properties of a strain of Pseudomonas stutzeri. , 2013, Chemosphere.

[21]  Yushu Ma,et al.  Phenanthrene biodegradation by an indigenousPseudomonas sp. ZJF08 with TX100 as surfactant , 2008, Annals of Microbiology.

[22]  S. Govindwar,et al.  Diesel and Kerosene Degradation by Pseudomonas desmolyticum NCIM 2112 and Nocardia hydrocarbonoxydans NCIM 2386 , 2008, Current Microbiology.

[23]  J. Gu,et al.  Degradation of indole by enrichment culture and Pseudomonas aeruginosa Gs isolated from mangrove sediment , 2005 .

[24]  A. Mukherjee,et al.  Crude petroleum-oil biodegradation efficiency of Bacillus subtilis and Pseudomonas aeruginosa strains isolated from a petroleum-oil contaminated soil from North-East India. , 2007, Bioresource technology.

[25]  J. Ramos,et al.  Survival in soils of an herbicide-resistant Pseudomonas putida strain bearing a recombinant TOL plasmid , 1991, Applied and environmental microbiology.

[26]  N. Desnoues,et al.  Alkane biodegradation in Pseudomonas aeruginosa strains isolated from a polluted zone: identification of alkB and alkB-related genes. , 2002, Research in microbiology.

[27]  V. L. Cardoso,et al.  Biodegradation of effluent contaminated with diesel fuel and gasoline. , 2007, Journal of hazardous materials.

[28]  Jo‐Shu Chang,et al.  Rhamnolipid production by indigenous Pseudomonas aeruginosa J4 originating from petrochemical wastewater , 2005 .

[29]  N. El-Gendy,et al.  Kinetic Modeling of the Bioremediation of Diesel Oil Polluted Seawater Using Pseudomonas aeruginosa NH1 , 2015 .

[30]  T. Vogel,et al.  Characterization of a soil bacterial consortium capable of degrading diesel fuel , 1999 .

[31]  M. Ilori,et al.  Differential degradation of crude oil (Bonny Light) by four Pseudomonas strains. , 2009, Journal of environmental sciences.

[32]  Sheng-Shung Cheng,et al.  Application of biosurfactants, rhamnolipid, and surfactin, for enhanced biodegradation of diesel-contaminated water and soil. , 2008, Journal of hazardous materials.