Lipase B from Candida antarctica — the wide applicable biocatalyst in obtaining pharmaceutical compounds

Lipases are commonly applied in the pharmaceutical and chemical industry, especially in immobilized form. The use of immobilized lipases facilitates the design of reactors and control of reactions, for example, fast stopping the reaction. The immobilization procedure should increase the stability of the lipase and its activity, as well as be simple and efficient. Lipase B from Candida antarctica (CAL-B) is an enzyme from the lipase group, isolated from the Candida antarctica species. CAL-B has the highest activity in non-polar organic solvents, such as hexane and toluene, and the lowest in polar solvents, e.g. acetonitrile. Due to its hydrolytic properties, this enzyme degrades triglycerides of fatty acids to free fatty acids (FFA) and glycerol. Described lipase is often immobilized, in the aim to increase enantioselective and lipolytic activity. The kinetic and dynamic resolution with the application of lipase is one of the ways in obtaining an enantiopure form of the drugs, which usually are more effective and safer for the patient. The CAL-B could be also applied in the kinetic resolution of compounds being building blocks, derivates of drugs or conjugated forms. Furthermore, the CAL-B is used in the reactions in receiving of organic compounds, which are the natural origin, especially vegetable. Based on the presented data, it can be concluded, that CAL-B is an enzyme with a wide application in the biosynthesis of compounds with therapeutic activity.

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