Investigation on hydrogen production from paper sludge without inoculation and its enhancement by Clostridium thermocellum.

The feasibility and performance of hydrogen production from paper sludge without inoculation was investigated under thermophilic conditions. The maximum hydrogen production reached 64.32 mM with 7.4% PS. The dynamic changes in bacterial community structures during hydrogen production were investigated by analyzing 16S rDNA gene sequences using high throughput sequencing technology. The results showed that microbial community was dominated by order Clostridiales and Thermoanaerobacterales. Genus Thermoanaerobacterium and Ruminiclostridium played a leading role in the fermentation process, which was responsible for the hydrolysis of PS and hydrogen production. Effect of inoculation with Clostridium thermocellum on hydrogen production from PS was also studied. The results showed that C. thermocellum supplement significantly increased hydrogen yield and holocellulose degradation rate by 96.80% and 32.95%, respectively. In addition, inoculation of C. thermocellum enhanced VFA generation and shortened the lag phase of hydrogen production. The present study lays the foundation on the valorization of waste lignocellulose.

[1]  R. Ferrari,et al.  Inhibitory effects of substrate and soluble end products on biohydrogen production of the alkalithermophile Caloramator celer: Kinetic, metabolic and transcription analyses , 2014 .

[2]  N. Ren,et al.  Characteristics of rumen microorganisms involved in anaerobic degradation of cellulose at various pH values , 2017 .

[3]  Weiyun Zhu,et al.  Characterising the bacterial microbiota across the gastrointestinal tracts of dairy cattle: membership and potential function , 2015, Scientific Reports.

[4]  J. Rintala,et al.  Thermophilic anaerobic digestion of pulp and paper mill primary sludge and co-digestion of primary and secondary sludge. , 2012, Water research.

[5]  Jingwei Ma,et al.  A simple methodology for rate-limiting step determination for anaerobic digestion of complex substrates and effect of microbial community ratio. , 2013, Bioresource technology.

[6]  Ming-Jun Zhu,et al.  High-yield biohydrogen production from non-detoxified sugarcane bagasse: Fermentation strategy and mechanism , 2018 .

[7]  Ming-jun Zhu,et al.  A novel anaerobic co-culture system for bio-hydrogen production from sugarcane bagasse. , 2013, Bioresource technology.

[8]  N. Ren,et al.  Inhibitory effects of acetate and ethanol on biohydrogen production of Ethanoligenens harbinese B49 , 2012 .

[9]  Tinghong Chang,et al.  Thermophilic, lignocellulolytic bacteria for ethanol production: current state and perspectives , 2011, Applied Microbiology and Biotechnology.

[10]  Huanzi Zhong,et al.  Thermophilic microbial cellulose decomposition and methanogenesis pathways recharacterized by metatranscriptomic and metagenomic analysis , 2014, Scientific Reports.

[11]  A. Tawfik,et al.  Dry anaerobic co-digestion of organic fraction of municipal waste with paperboard mill sludge and gelatin solid waste for enhancement of hydrogen production. , 2015, Bioresource technology.

[12]  Robert C. Edgar,et al.  UPARSE: highly accurate OTU sequences from microbial amplicon reads , 2013, Nature Methods.

[13]  A. E. Greenberg,et al.  Standard methods for the examination of water and wastewater : supplement to the sixteenth edition , 1988 .

[14]  Samir Kumar Khanal,et al.  Kinetic study of biological hydrogen production by anaerobic fermentation , 2006 .

[15]  Tong Zhang,et al.  Biohydrogen production from starch in wastewater under thermophilic condition. , 2003, Journal of environmental management.

[16]  F. Widdel.  The Genus Desulfotomaculum , 2006 .

[17]  K. Haga,et al.  Effect of fermentation temperature on hydrogen production from cow waste slurry by using anaerobic microflora within the slurry , 2007, Applied Microbiology and Biotechnology.

[18]  Lei Liang,et al.  Enhanced biohydrogen production from sugarcane bagasse by Clostridium thermocellum supplemented with CaCO3. , 2015, Bioresource technology.

[19]  Ming-Jun Zhu,et al.  Evaluation of spent mushroom compost as a lignocellulosic substrate for hydrogen production by Clostridium thermocellum , 2017 .

[20]  R. Sparling,et al.  Hydrogen, ethanol and cellulase production from pulp and paper primary sludge by fermentation with Clostridium thermocellum , 2015 .

[21]  Aijie Wang,et al.  Direct hydrogen production from lignocellulose by the newly isolated Thermoanaerobacterium thermosaccharolyticum strain DD32 , 2015 .

[22]  Shubin Wu,et al.  Hydrogen-methane production from pulp & paper sludge and food waste by mesophilic–thermophilic anaerobic co-digestion , 2013 .

[23]  Jiajie Zhang,et al.  PEAR: a fast and accurate Illumina Paired-End reAd mergeR , 2013, Bioinform..

[24]  B. Henrissat,et al.  Genomic Evaluation of Thermoanaerobacter spp. for the Construction of Designer Co-Cultures to Improve Lignocellulosic Biofuel Production , 2013, PloS one.

[25]  Amie D. Sluiter,et al.  Determination of Structural Carbohydrates and Lignin in Biomass , 2004 .

[26]  F. Girio,et al.  Conversion of recycled paper sludge to ethanol by SHF and SSF using Pichia stipitis , 2008 .

[27]  Hang-Sik Shin,et al.  Hydrogen fermentation of food waste without inoculum addition , 2009 .

[28]  Yuan Lu,et al.  Characteristics of hydrogen and methane production from cornstalks by an augmented two- or three-stage anaerobic fermentation process. , 2009, Bioresource technology.

[29]  Xiaoqian Ma,et al.  The thermal behaviour of the co-combustion between paper sludge and rice straw. , 2013, Bioresource technology.

[30]  S. Campanaro,et al.  Bioaugmentation with hydrolytic microbes to improve the anaerobic biodegradability of lignocellulosic agricultural residues. , 2017, Bioresource technology.

[31]  Andreas Poullikkas,et al.  A comparative overview of hydrogen production processes , 2017 .

[32]  J. Wiegel,et al.  Clostridium thermobutyricum: growth studies and stimulation of butyrate formation by acetate supplementation. , 2002, Microbiological research.

[33]  Irini Angelidaki,et al.  Thermophilic fermentative hydrogen production by the newly isolated Thermoanaerobacterium thermosaccharolyticum PSU-2 , 2008 .

[34]  Zsófia Kádár,et al.  Yields from glucose, xylose, and paper sludge hydrolysate during hydrogen production by the extreme thermophile Caldicellulosiruptor saccharolyticus , 2004, Applied biochemistry and biotechnology.

[35]  T. Meyer,et al.  Anaerobic digestion of pulp and paper mill wastewater and sludge. , 2014, Water research.

[36]  Richard J. Giannone,et al.  Profile of Secreted Hydrolases, Associated Proteins, and SlpA in Thermoanaerobacterium saccharolyticum during the Degradation of Hemicellulose , 2014, Applied and Environmental Microbiology.

[37]  F. Widdel,et al.  Growth with hydrogen, and further physiological characteristics of Desulfotomaculum species , 1985, Archives of Microbiology.

[38]  T. Beppu,et al.  Clostridium clariflavum sp. nov. and Clostridium caenicola sp. nov., moderately thermophilic, cellulose-/cellobiose-digesting bacteria isolated from methanogenic sludge. , 2009, International journal of systematic and evolutionary microbiology.

[39]  Orhan Ince,et al.  Bioaugmentation with Clostridium thermocellum to enhance the anaerobic biodegradation of lignocellulosic agricultural residues. , 2018, Bioresource technology.

[40]  Shiwen Fang,et al.  Thermogravimetric analysis of the co-pyrolysis of paper sludge and municipal solid waste , 2015 .

[41]  C. Hansen,et al.  Optimization of anaerobic hydrogen and methane production from dairy processing waste using a two-stage digestion in induced bed reactors (IBR) , 2015 .

[42]  Debabrata Das,et al.  Biohydrogen production by dark fermentation , 2013 .

[43]  Sheng-Shung Cheng,et al.  Bioaugmentation Strategies to Improve Cellulolytic and Hydrogen Producing Characteristics in CSTR Intermittent Fed with Vegetable Kitchen Waste and Napiergrass , 2012 .

[44]  C. Cavinato,et al.  Changes in microbial community during hydrogen and methane production in two-stage thermophilic anaerobic co-digestion process from biowaste. , 2016, Waste management.

[45]  J. Blanchard,et al.  Untangling the Genetic Basis of Fibrolytic Specialization by Lachnospiraceae and Ruminococcaceae in Diverse Gut Communities , 2013 .

[46]  E. Bonch‐Osmolovskaya,et al.  Mobilitalea sibirica gen. nov., sp. nov., a halotolerant polysaccharide-degrading bacterium. , 2014, International journal of systematic and evolutionary microbiology.