Phase resolving microscopy by multi‐plane diffraction detection

An easily applicable quantitative phase‐contrast microscopy is presented. A bright‐field microscope with a coherent illumination is used. Only a series of diffraction images is taken in different distances from the sample. For reconstruction, a conjugate gradient technique as a new variant of phase retrieval technique is developed. The technique is experimentally investigated in detail. A phase accuracy up to 0.07 rad or a height accuracy of 13 nm in transmission for a binary test sample is reached, respectively.

[1]  Thomas J. Flynn,et al.  TWO-DIMENSIONAL PHASE UNWRAPPING WITH MINIMUM WEIGHTED DISCONTINUITY , 1997 .

[2]  James C. Wyant,et al.  White light interferometry , 2002, SPIE Defense + Commercial Sensing.

[3]  Sharon V. King,et al.  Algorithms for extracting true phase from rotationally-diverse and phase-shifted DIC images , 2006, SPIE BiOS.

[4]  G. Pedrini,et al.  Complete wavefront reconstruction using sequential intensity measurements of a volume speckle field. , 2006, Applied optics.

[5]  R. Gerchberg A practical algorithm for the determination of phase from image and diffraction plane pictures , 1972 .

[6]  E. Cuche,et al.  Characterization of microlenses by digital holographic microscopy. , 2006, Applied optics.

[7]  D. Gabor Microscopy by reconstructed wave-fronts , 1949, Proceedings of the Royal Society of London. Series A. Mathematical and Physical Sciences.

[8]  B Y Gu,et al.  Gerchberg-Saxton and Yang-Gu algorithms for phase retrieval in a nonunitary transform system: a comparison. , 1994, Applied optics.

[9]  P. Scott,et al.  Phase retrieval and twin-image elimination for in-line Fresnel holograms , 1987 .

[10]  F. A. Seiler,et al.  Numerical Recipes in C: The Art of Scientific Computing , 1989 .

[11]  Wolfgang Osten,et al.  Image reconstruction for in-line holography with the Yang-Gu algorithm. , 2003, Applied optics.

[12]  G. Pedrini,et al.  Wave-front reconstruction from a sequence of interferograms recorded at different planes. , 2005, Optics letters.

[13]  Daniel Carl,et al.  Parameter-optimized digital holographic microscope for high-resolution living-cell analysis. , 2004, Applied optics.

[14]  Lenseless coherent imaging by multi-plane interference detection , 2005 .

[15]  Chun-Min Lo,et al.  High-resolution quantitative phase-contrast microscopy by digital holography. , 2005, Optics express.