Production of conjugated linoleic acids by Lactobacillus plantarum strains isolated from naturally fermented Chinese pickles

Naturally fermented pickles harbour many lactic acid bacteria (LAB). Forty-three LAB strains with conjugated linoleic acid (CLA)-producing ability were isolated from three naturally fermented pickle brines. Of these isolates, lp15 identified as Lactobacillus plantarum by API 50 CHL system and full-length 16S rDNA sequence analysis exhibited the highest CLA-producing ability (26.1% conversion) at 48 h in de Man Rogosa Sharpe (MRS) broth in the presence of 100 μg/ml of linoleic acid (LA). Compared to other strains, L. plantarum strain lp15 showed the highest tolerance upon increased levels of LA in the medium, i.e., up to 600 μg/ml. This strain converted about 25% of LA into CLA isomers [predominantly cis-9, trans-11 CLA (9-CLA) and trans-10, cis-12 CLA (10-CLA)], of which 75% was 9-CLA. Interestingly, though the conversion rate of LA into CLA by lp15 remained stable between 100 to 600 μg/ml LA levels in the medium, it dropped sharply at 1000 μg/ml. Taken together, the lp15 strain displayed relatively high LA tolerance with higher conversion rate, which implies that this strain is a valuable candidate for enhancing the CLA content in food-sources like pickles.

[1]  G. He,et al.  Construction of the yeast whole-cell Rhizopus oryzae lipase biocatalyst with high activity , 2011, Journal of Zhejiang University SCIENCE B.

[2]  G. He,et al.  A food-grade industrial arming yeast expressing β-1,3-1,4-glucanase with enhanced thermal stability , 2010, Journal of Zhejiang University SCIENCE B.

[3]  F. Spener,et al.  Conjugated linoleic acids as functional food: an insight into their health benefits , 2009, Nutrition & metabolism.

[4]  M. M. Rahman,et al.  Effect of CLA isomers and their mixture on aging C57Bl/6J mice , 2009, European journal of nutrition.

[5]  D. Gong,et al.  Identification of lactic acid bacterial strains with high conjugated linoleic acid-producing ability from natural sauerkraut fermentations. , 2009, Journal of food science.

[6]  G. Kaban,et al.  Identification of lactic acid bacteria and Gram-positive catalase-positive cocci isolated from naturally fermented sausage (sucuk). , 2008, Journal of food science.

[7]  R. Kreider,et al.  Conjugated Linoleic Acids , 2008, Current sports medicine reports.

[8]  R. McLeod,et al.  Conjugated linoleic acid and atherosclerosis: studies in animal models. , 2008, Biochemistry and cell biology = Biochimie et biologie cellulaire.

[9]  H. Park,et al.  Synthesis of conjugated linoleic acid by human-derived Bifidobacterium breve LMC 017: utilization as a functional starter culture for milk fermentation. , 2008, Journal of agricultural and food chemistry.

[10]  R. Wall,et al.  Microbial conjugated linoleic acid production - a novel probiotic trait? , 2008 .

[11]  K. Erickson,et al.  Conjugated linoleic acid isomers and cancer. , 2007, The Journal of nutrition.

[12]  H. P. Fleming,et al.  DNA Fingerprinting of Lactic Acid Bacteria in Sauerkraut Fermentations , 2007, Applied and Environmental Microbiology.

[13]  S. González,et al.  Conjugated linoleic acid conversion by dairy bacteria cultured in MRS broth and buffalo milk , 2007, Letters in applied microbiology.

[14]  E. Drosinos,et al.  Phenotypic and technological diversity of lactic acid bacteria and staphylococci isolated from traditionally fermented sausages in southern Greece. , 2007, Food microbiology.

[15]  M. Deng,et al.  Purification and characterization of a membrane-bound linoleic acid isomerase from Clostridium sporogenes , 2007 .

[16]  G. Fitzgerald,et al.  Rapid Screening Method for Analyzing the Conjugated Linoleic Acid Production Capabilities of Bacterial Cultures , 2007, Applied and Environmental Microbiology.

[17]  Manuel Hernández,et al.  Identification and differentiation of Lactobacillus, Streptococcus and Bifidobacterium species in fermented milk products with bifidobacteria. , 2007, Microbiological research.

[18]  G. Fernandes,et al.  Biological effects of conjugated linoleic acids in health and disease. , 2006, The Journal of nutritional biochemistry.

[19]  S. Kishino,et al.  Production of conjugated fatty acids by lactic acid bacteria. , 2005, Journal of bioscience and bioengineering.

[20]  D. Bauman,et al.  Short communication: effect of production variables on the cis-9, trans-11 conjugated linoleic acid content of cows' milk. , 2005, Journal of dairy science.

[21]  T. Yanagita,et al.  Conjugated fatty acids in food and their health benefits. , 2005, Journal of bioscience and bioengineering.

[22]  B. Martín,et al.  Microbial Quality and Direct PCR Identification of Lactic Acid Bacteria and Nonpathogenic Staphylococci from Artisanal Low-Acid Sausages , 2005, Applied and Environmental Microbiology.

[23]  T. Yanagita,et al.  Conjugated linoleic acid prevents the development of essential hypertension in spontaneously hypertensive rats. , 2004, Biochemical and biophysical research communications.

[24]  S. Seseña,et al.  Polyphasic study of the genetic diversity of lactobacilli associated with ‘Almagro’ eggplants spontaneous fermentation, based on combined numerical analysis of randomly amplified polymorphic DNA and pulsed‐field gel electrophoresis patterns , 2004, Journal of applied microbiology.

[25]  S. Gilliland,et al.  Production of free conjugated linoleic acid by Lactobacillus acidophilus and Lactobacillus casei of human intestinal origin. , 2003, Journal of dairy science.

[26]  M. Coakley,et al.  Conjugated linoleic acid biosynthesis by human‐derived Bifidobacterium species , 2003, Journal of applied microbiology.

[27]  T. Iwashita,et al.  Structural Analysis of Conjugated Linoleic Acid Produced by Lactobacillus plantarum, and Factors Affecting Isomer Production , 2003, Bioscience, biotechnology, and biochemistry.

[28]  J. Martin,et al.  Conjugated linoleic acids: all the same or to everyone its own function? , 2002, Reproduction, nutrition, development.

[29]  J. A. Kelsey,et al.  Analysis of variation in cis-9, trans-11 conjugated linoleic acid (CLA) in milk fat of dairy cows. , 2002, Journal of dairy science.

[30]  M. Cook,et al.  The biologically active isomers of conjugated linoleic acid. , 2001, Progress in lipid research.

[31]  D E Bauman,et al.  Effect of dietary lipid source on conjugated linoleic acid concentrations in milk fat. , 2001, Journal of dairy science.

[32]  R. Liu,et al.  Effect of Linoleic Acid Concentration on Conjugated Linoleic Acid Production by Butyrivibrio fibrisolvensA38 , 2000, Applied and Environmental Microbiology.

[33]  D. Schingoethe,et al.  Influence of dietary fish oil on conjugated linoleic acid and other fatty acids in milk fat from lactating dairy cows. , 2000, Journal of dairy science.

[34]  D. Palmquist,et al.  Short communication: influence of dietary tallow and fish oil on milk fat composition. , 2000, Journal of dairy science.

[35]  K. Yuen,et al.  Identification by 16S ribosomal RNA gene sequencing of an Enterobacteriaceae species from a bone marrow transplant recipient , 2000, Molecular pathology : MP.

[36]  H. Macdonald.  Conjugated Linoleic Acid and Disease Prevention: A Review of Current Knowledge , 2000, Journal of the American College of Nutrition.

[37]  T. Lin,et al.  Conjugated linoleic acid concentration as affected by lactic cultures and added linoleic acid , 1999 .

[38]  Yeonhwa Park,et al.  Evidence that the trans-10,cis-12 isomer of conjugated linoleic acid induces body composition changes in mice , 1999, Lipids.

[39]  Björck,et al.  Production of conjugated linoleic acid by dairy starter cultures , 1998, Journal of applied microbiology.

[40]  M. Pariza,et al.  Anticarcinogens from fried ground beef: heat-altered derivatives of linoleic acid. , 1987, Carcinogenesis.

[41]  Robert Johnson,et al.  Structural Analysis , 2020, Multiphysics Modeling with Application to Biomedical Engineering.

[42]  H. Schöneberger,et al.  [Phenotypic and technological influences of the Lupinus mutabilis (Tarwi) seed on its methionine availability and sulfur content]. , 1983, Archivos latinoamericanos de nutricion.

[43]  M. Portillo,et al.  Conjugated linoleic acid isomers: Differences in metabolism and biological effects , 2009, BioFactors.

[44]  B. G. Ribeiro,et al.  Conjugated linoleic acid (CLA): effect modulation of body composition and lipid profile. , 2009, Nutricion hospitalaria.

[45]  S. Y. Park,et al.  Technical note: Improved extraction method with hexane for gas chromatographic analysis of conjugated linoleic acids. , 2006, Journal of dairy science.

[46]  K. Yuen,et al.  Identification by 16 S ribosomal RNA gene sequencing of an Enterobacteriaceae species from a bone marrow transplant recipient , 2000 .

[47]  S. Surzycki.  Preparation of Genomic DNA from Bacteria , 2000 .

[48]  B. Watkins,et al.  Biochemical and Molecular Roles of Nutrients Dietary Conjugated Linoleic Acid Influences the Immune Response of Young and Old C 57 BL / 6 NCrlBR Mice . 1 , 2 , 1999 .

[49]  B. Watkins,et al.  Dietary conjugated linoleic acid influences the immune response of young and old C57BL/6NCrlBR mice. , 1999, The Journal of nutrition.