Silicate bioceramics elicit proliferation and odonto-genic differentiation of human dental pulp cells.

This study aimed to investigate the effects of silicates on the proliferation and odontogenic differentiation of human dental pulp cells (hDPCs) in vitro. HDPCs were cultured in the presence of calcium silicate (CS) extracts, while calcium hydroxide (CH) extracts and culture medium without CH or CS were used as the control groups. The calcium and phosphorus ion concentrations in the CS were similar to those in the control groups, but the concentration of silicon ions in the CS extracts was higher than that in the control groups. HDPCs cultured with CS and CH extracts at dilution of 1/128 proliferated significantly more than those cultured with the control treatments. CS extracts promoted cell migration, enhanced the expression of odontogenic marker genes and conspicuously increased odontogenesis-related protein production and the release of cytokines, suggesting that CS bioactive ceramics possess excellent biocompatibility and bioactivity and have the potential for application as pulp-capping agents.

[1]  X. Liu,et al.  Cell migration and osteo/odontogenesis stimulation of iRoot FS as a potential apical barrier material in apexification. , 2020, International endodontic journal.

[2]  W. Shi,et al.  Short lipopeptides specifically inhibit the growth of Propionibacterium acnes with a dual antibacterial and anti‐inflammatory action , 2019, British journal of pharmacology.

[3]  Ya Shen,et al.  Effects of two fast-setting pulp-capping materials on cell viability and osteogenic differentiation in human dental pulp stem cells: An in vitro study. , 2019, Archives of oral biology.

[4]  F. Schwendicke,et al.  Effects of calcium silicate cements on dental pulp cells: A systematic review. , 2018, Journal of dentistry.

[5]  Jiang Chang,et al.  Effects of silicate-based composite material on the proliferation and mineralization behaviors of human dental pulp cells: An in vitro assessment. , 2018, Dental materials journal.

[6]  K. Tanimoto,et al.  Comparative characterization of stem cells from human exfoliated deciduous teeth, dental pulp, and bone marrow-derived mesenchymal stem cells. , 2018, Biochemical and biophysical research communications.

[7]  T. Ishimoto,et al.  Novel evaluation method of dentin repair by direct pulp capping using high-resolution micro-computed tomography , 2018, Clinical Oral Investigations.

[8]  M. Jeon,et al.  Cytokine Expression of Stem Cells Originating from the Apical Complex and Coronal Pulp of Immature Teeth , 2018, Journal of endodontics.

[9]  D. Kaigler,et al.  Dental Pulp Tissue Regeneration Using Dental Pulp Stem Cells Isolated and Expanded in Human Serum , 2017, Journal of endodontics.

[10]  B. A. Rodas-Junco,et al.  Dental Pulp Stem Cells: Current Advances in Isolation, Expansion and Preservation , 2017, Tissue Engineering and Regenerative Medicine.

[11]  M. Warhadpande,et al.  A comparison of human dental pulp response to calcium hydroxide and Biodentine as direct pulp-capping agents , 2017, Journal of conservative dentistry : JCD.

[12]  B. Suh,et al.  Cytotoxicity and biocompatibility of resin-free and resin-modified direct pulp capping materials: A state-of-the-art review. , 2017, Dental materials journal.

[13]  S. Rawlinson,et al.  Strontium (Sr) elicits odontogenic differentiation of human dental pulp stem cells (hDPSCs): A therapeutic role for Sr in dentine repair? , 2016, Acta biomaterialia.

[14]  M. Gandolfi,et al.  Calcium silicate bioactive cements: Biological perspectives and clinical applications. , 2015, Dental Materials.

[15]  C. Kao,et al.  Odontogenic differentiation of human dental pulp cells by calcium silicate materials stimulating via FGFR/ERK signaling pathway. , 2014, Materials science & engineering. C, Materials for biological applications.

[16]  B. Peng,et al.  A comparative study of BioAggregate and ProRoot MTA on adhesion, migration, and attachment of human dental pulp cells. , 2014, Journal of endodontics.

[17]  Jiang Chang,et al.  Silicate bioceramics enhanced vascularization and osteogenesis through stimulating interactions between endothelia cells and bone marrow stromal cells. , 2014, Biomaterials.

[18]  Xiaofeng Chen,et al.  Ionic extraction of a novel nano-sized bioactive glass enhances differentiation and mineralization of human dental pulp cells. , 2014, Journal of endodontics.

[19]  Jiang Chang,et al.  The calcium silicate/alginate composite: preparation and evaluation of its behavior as bioactive injectable hydrogels. , 2013, Acta biomaterialia.

[20]  J. Chang,et al.  Bioactive silicate materials stimulate angiogenesis in fibroblast and endothelial cell co-culture system through paracrine effect. , 2013, Acta biomaterialia.

[21]  M. Gandolfi,et al.  Chemical-physical properties of TheraCal, a novel light-curable MTA-like material for pulp capping. , 2012, International endodontic journal.

[22]  M. Shie,et al.  Effects of altering the Si/Ca molar ratio of a calcium silicate cement on in vitro cell attachment. , 2012, International endodontic journal.

[23]  Paul A. Rosenberg,et al.  Repair and regeneration in endodontics. , 2011, International endodontic journal.

[24]  Jiang Chang,et al.  Effect of tricalcium silicate on the proliferation and odontogenic differentiation of human dental pulp cells. , 2011, Journal of endodontics.

[25]  P. Dummer,et al.  Properties and applications of calcium hydroxide in endodontics and dental traumatology. , 2011, International endodontic journal.

[26]  V. D’antò,et al.  Effect of mineral trioxide aggregate on mesenchymal stem cells. , 2010, Journal of endodontics.

[27]  Changsheng Liu,et al.  An in vitro investigation of the mechanical-chemical and biological properties of calcium phosphate/calcium silicate/bismutite cement for dental pulp capping. , 2010, Journal of biomedical materials research. Part B, Applied biomaterials.

[28]  Hai Zhang,et al.  Effects of mineral trioxide aggregate on human dental pulp cells after pulp-capping procedures. , 2010, Journal of endodontics.

[29]  M. Torabinejad,et al.  Mineral trioxide aggregate: a comprehensive literature review--Part III: Clinical applications, drawbacks, and mechanism of action. , 2010, Journal of endodontics.

[30]  J. Chung,et al.  Dental stem cell therapy with calcium hydroxide in dental pulp capping. , 2010, Tissue Engineering. Part A.

[31]  M. Torabinejad,et al.  Mineral trioxide aggregate: a comprehensive literature review--part II: leakage and biocompatibility investigations. , 2010, Journal of endodontics.

[32]  J. Hebling,et al.  CYTOTOXICITY AND BIOCOMPATIBILITY OF DIRECT AND INDIRECT PULP CAPPING MATERIALS , 2009, Journal of applied oral science : revista FOB.

[33]  G. T. Huang,et al.  Apexification: the beginning of its end. , 2009, International endodontic journal.

[34]  Jiang Chang,et al.  Effects of Wollastonite on Proliferation and Differentiation of Human Bone Marrow-derived Stromal Cells in PHBV/Wollastonite Composite Scaffolds , 2009, Journal of biomaterials applications.

[35]  A. Kulkarni,et al.  DSPP effects on in vivo bone mineralization. , 2008, Bone.

[36]  H. Duncan,et al.  Histological, ultrastructural and quantitative investigations on the response of healthy human pulps to experimental capping with mineral trioxide aggregate: a randomized controlled trial , 2007, BDJ.

[37]  E. Scheller,et al.  Wnt/beta-catenin inhibits dental pulp stem cell differentiation. , 2008, Journal of dental research.

[38]  J. K. Leach,et al.  Proangiogenic Potential of a Collagen/Bioactive Glass Substrate , 2008, Pharmaceutical Research.

[39]  A. George,et al.  Dentin Matrix Protein 1 Regulates Dentin Sialophosphoprotein Gene Transcription during Early Odontoblast Differentiation* , 2006, Journal of Biological Chemistry.

[40]  M. Itoh,et al.  FGF-2 potently induces both proliferation and DSP expression in collagen type I gel cultures of adult incisor immature pulp cells. , 2004, Biochemical and biophysical research communications.

[41]  Bingshuang Zou,et al.  Differential expression patterns of the dentin matrix proteins during mineralized tissue formation. , 2004, Bone.

[42]  M. Ghanbariha,et al.  Mineral trioxide aggregate (MTA) and calcium hydroxide as pulp-capping agents in human teeth: a preliminary report. , 2003, International endodontic journal.

[43]  A. George,et al.  Differentiation of embryonic mesenchymal cells to odontoblast-like cells by overexpression of dentin matrix protein 1 , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[44]  M. Rafter.  Vital pulp therapy--a review. , 2001, Journal of the Irish Dental Association.

[45]  I. About,et al.  Human dentin production in vitro. , 2000, Experimental cell research.

[46]  T. Kawamoto,et al.  Differential effects of various growth factors and cytokines on the syntheses of DNA, type I collagen, laminin, fibronectin, osteonectin/secreted protein, acidic and rich in cysteine (SPARC), and alkaline phosphatase by human pulp cells in culture , 1998, Journal of cellular physiology.

[47]  D. Simmons,et al.  Dentin matrix protein-1, a candidate gene for dentinogenesis imperfecta. , 1996, Connective tissue research.

[48]  D. Tziafas.  Basic mechanisms of cytodifferentiation and dentinogenesis during dental pulp repair. , 1995, The International journal of developmental biology.