Chemical forms of aluminum in xylem sap of tea plants (Camellia sinensis L.).

To identify the chemical forms of aluminum (Al) transported from roots to shoots of tea plants (C. sinensis L.), 27Al-nuclear magnetic resonance and 19F NMR spectroscopy were used to analyze xylem sap. The concentration of Al in collected xylem sap was 0.29 mM, twice as high as that of F. Catechins were not detected in xylem sap. The concentration of malic acid in xylem sap was higher than that of citric acid, whereas the concentration of oxalic acid was negligible. There were two signals in the 27Al NMR spectra of xylem sap, a larger signal at 11 ppm and a smaller one at -1.5 ppm. The former signal was consistent with the peak for an Al-citrate model solution, suggesting that an Al-citrate complex was present in xylem sap. Although the latter signal at -1.5 ppm was thought to indicate the presence of an Al-F complex (at 1.7 ppm) in xylem sap, there was only one signal at -122 ppm in the 19F NMR spectrum of xylem sap, indicating that the main F complex in xylem sap was F-. These results indicate that Al might be translocated as a complex with citrate, while Al-malate, Al-oxalate and Al-F complexes are not major Al complexes in xylem sap of tea plants.

[1]  J. F. Ma,et al.  Role of organic acids in detoxification of aluminum in higher plants. , 2000, Plant & cell physiology.

[2]  Yuko Yoshida,et al.  Simultaneous analysis of individual catechins and caffeine in green tea , 1996 .

[3]  G. Kerven,et al.  Quantitative 27Al NMR spectroscopic studies of Al (III) complexes with organic acid ligands and their comparison with GEOCHEM predicted values , 2004, Plant and Soil.

[4]  H. Matsumoto,et al.  Localization of aluminium in tea leaves , 1976 .

[5]  Ma.,et al.  Oxalate exudation by taro in response to Al , 1998, Plant physiology.

[6]  M. Hayatsu,et al.  Identification of aluminium forms in tea leaves by 27Al NMR , 1992 .

[7]  G. J. Taylor.  Current views of the aluminum stress response; the physiological basis of tolerance. , 1991 .

[8]  W. Lukiw,et al.  New Evidence for an Active Role of Aluminum in Alzheimer's Disease , 1989, Canadian Journal of Neurological Sciences / Journal Canadien des Sciences Neurologiques.

[9]  G. W. Thomas,et al.  Quantitative determination of aluminum-27 by high-resolution nuclear magnetic resonance spectrometry , 1986 .

[10]  M. Hayatsu,et al.  Aluminium kinetics in the tea plant using 27Al and 19F NMR , 1993 .

[11]  T. Iwashita,et al.  Internal Detoxification Mechanism of Al in Hydrangea (Identification of Al Form in the Leaves) , 1997, Plant physiology.

[12]  B. Pihlar,et al.  The uptake and speciation of various Al species in the Brassica rapa pekinensis. , 2001, Phytochemistry.

[13]  S. Zheng,et al.  Detoxifying aluminium with buckwheat , 1997, Nature.

[14]  E. M. Chenery.  A preliminary study of aluminium and the tea bush , 1955, Plant and Soil.

[15]  E. Delhaize,et al.  Aluminium tolerance in plants and the complexing role of organic acids. , 2001, Trends in plant science.

[16]  O. Talibudeen,et al.  Effect of aluminium on growth of tea (Camellia sinensis) and its uptake of potassium and phosphorus , 1971 .

[17]  B. Pihlar,et al.  Quantitative determination of trace amounts of Al-citrate by anion-exchange FPLC-ETAAS. , 1998, Talanta.

[18]  Toshiaki Tadano,et al.  Distribution and chemical speciation of aluminum in the Al accumulator plant, Melastoma malabathricum L. , 1998, Plant and Soil.

[19]  A. Morita,et al.  Nitrate and oxalate contents of tea plants (Camellia sinensis L.) with special reference to types of green tea and effect of shading , 2002 .

[20]  S. Hiradate,et al.  Form of aluminium for uptake and translocation in buckwheat (Fagopyrum esculentum Moench) , 2000, Planta.

[21]  R. J. Williams,et al.  What is wrong with aluminium? The J.D. Birchall Memorial Lecture. , 1999, Journal of inorganic biochemistry.

[22]  R. Chaney,et al.  The Physiology of Metal Toxicity in Plants , 1978 .

[23]  M. Osaki,et al.  Influence of aluminum and phosphorus on growth and xylem sap composition in Melastoma malabathricum L. , 2001, Plant and Soil.

[24]  D. Perl,et al.  Alzheimer's disease: X-ray spectrometric evidence of aluminum accumulation in neurofibrillary tangle-bearing neurons. , 1980, Science.

[25]  B. Pihlar,et al.  Combination of various analytical techniques for speciation of low molecular weight aluminium complexes in plant sap , 1999 .

[26]  L. Kochian.  Cellular Mechanisms of Aluminum Toxicity and Resistance in Plants , 1995 .