Evaluation of Genetic Diversity of QPM, Provit-A, and Elite Maize Inbreds Resistant to Downy Mildew Disease Using Simple Sequence Repeats

Functional maize tends to be more susceptible to major maize diseases, particularly Downy mildew. Among the functional maize are Quality Protein Maize (QPM) and Provit A maize. The presence of higher amino acid and beta carotene in functional maize might have caused these types of maize more susceptible to Downy mildew disease. The objective of the research was to identify the heterotic pairs among maize inbreds i.e. QPM, Provit A, and local maize varieties resistant to Downy mildew disease using Simple Sequence Repeats (SSR) marker. The research was conducted from April to July 2017 at the Molecular Biology Laboratory of Indonesian Cereals Research Institute. A total of five QPM inbreds, 15 Provit A inbreds, and 11 Downy mildew resistant local varieties of maize were used in the experiment using 34 SSR markers. Results indicated that among 34 SSR locus analysed, variation of allele lengths ranged from 74 bp to 500 bp. A total of 125 alleles ranging from two to nine alleles per locus with an average of 3.68 alleles were generated. The data indicated wide genetic variations among characters. DNA band profile showed that nc130 marker produced the highest PIC (over 0.83) and allele value (8.00). Genetic distance analysis found a total of 21 heterotic genotypes with genetic distance exceeds 0.65.

[1]  B. Daryono,et al.  Penanda Molekuler Inter Simple Sequence Repeat untuk Menentukan Ketahanan Tanaman Jagung terhadap Penyakit Bulai , 2017 .

[2]  A. Muis,et al.  Skrining Ketahanan Galur S1 Jagung terhadap Penyakit Bulai dan Pembentukan Galur S2 Tahan Penyakit Bulai , 2016 .

[3]  M. B. Pabendon,et al.  Keragaman Genetik Inbrida Jagung QPM dan Provit-A Berdasarkan Marka SSRs (Simple Sequence Repeats) , 2016 .

[4]  L. N. Tandzi,et al.  Molecular Characterization of Selected Maize ( zea mays l.) Inbred Lines , 2015 .

[5]  Shelton M. Charles,et al.  Genetic variation of selected quality protein maize inbred lines , 2015 .

[6]  I. Haș,et al.  Correction: Romanian Maize (Zea mays) Inbred Lines as a Source of Genetic Diversity in SE Europe, and Their Potential in Future Breeding Efforts , 2014, PLoS ONE.

[7]  J. A. Garzón-Tiznado,et al.  Microsatellite-based genetic diversity among accessions of maize landraces from Sinaloa in México. , 2013, Hereditas.

[8]  Yunbi Xu,et al.  Genetic Diversity and Molecular Evolution of Chinese Waxy Maize Germplasm , 2013, PloS one.

[9]  P. Grudloyma,et al.  Mapping of QTL affecting resistance against sorghum downy mildew (Peronosclerospora sorghi) in maize (Zea mays L) , 2013 .

[10]  G. Bronner,et al.  Positional cloning of a candidate gene for resistance to the sunflower downy mildew, Plasmopara halstedii race 300 , 2013, Theoretical and Applied Genetics.

[11]  P. Grudloyma,et al.  Detection and integration of gene mapping of downy mildew resistance in maize inbred lines though linkage and association , 2012, Euphytica.

[12]  Kyong-Cheul Park,et al.  Analysis of genetic mapping in a waxy/dent maize RIL population using SSR and SNP markers , 2012, Genes & Genomics.

[13]  M. Warburton,et al.  Genetic diversity of maize inbred lines in relation to downy mildew , 2004, Euphytica.

[14]  A. Nikolic,et al.  Genetic diversity of maize inbred lines as inferred from SSR markers , 2015 .

[15]  P. H. Zaidi,et al.  Downy mildew resistance in maize (Zea mays L.) across Peronosclerospora species in lowland tropical Asia , 2013 .

[16]  N. Choisne,et al.  Positional cloning of disease resistance genes in grapevine. , 2011 .