Tenascin expression in human chronic liver disease and in hepatocellular carcinoma.

Tenascin is an oligomeric glycoprotein of the extracellular matrix synthesized during embryonic development. It is prominently expressed in a variety of tumors. The role of tenascin in liver tissue is, however, unknown. We used immunocytochemistry to define the localization of tenascin and compare this with the localization of non-collagenous proteins, such as laminin and fibronectin, in normal human liver and pathological liver from patients with chronic hepatitis, liver cirrhosis and hepatocellular carcinoma. In normal liver, tenascin expression was localized along the sinusoidal and vascular wall. In fibrotic liver, tenascin was also observed in the region between the hepatic parenchyma and the fibrosing portal tracts, especially in areas of piecemeal necrosis in chronic hepatitis. Immuno-EM study of liver tissue in chronic hepatitis strongly suggested the synthesis and secretion of tenascin by fat-storing cells into the space of Disse. In hepatocellular carcinoma, tenascin was expressed in both the capsule and lobular septa, but not in the sinusoidal walls of the tumors. These results led us to postulate a close relationship between the occurrence of this protein and disease processes such as fibrosis and cancer invasion.

[1]  P. Amenta,et al.  The extracellular matrix in hepatic regeneration. Localization of collagen types I, III, IV, laminin, and fibronectin. , 1991, Laboratory investigation; a journal of technical methods and pathology.

[2]  P. van Eyken,et al.  Expression of the novel extracellular matrix component tenascin in normal and diseased human liver. An immunohistochemical study. , 1990, Journal of hepatology.

[3]  E. Wisse,et al.  Subcellular abnormalities of liver sinusoidal lesions in human hepatocellular carcinoma. , 1990, Journal of submicroscopic cytology and pathology.

[4]  M. Schachner,et al.  J1/tenascin-related molecules are not responsible for the segmented pattern of neural crest cells or motor axons in the chick embryo. , 1989, Development.

[5]  M. Colombo,et al.  Distribution of basement membrane components in human hepatocellular carcinoma , 1989, Cancer.

[6]  W. Halfter,et al.  Induction of tenascin in healing wounds , 1988, The Journal of cell biology.

[7]  M. Kusakabe,et al.  Epithelial induction of stromal tenascin in the mouse mammary gland: from embryogenesis to carcinogenesis. , 1988, Developmental biology.

[8]  W. Halfter,et al.  The distribution of fibronectin and tenascin along migratory pathways of the neural crest in the trunk of amphibian embryos. , 1988, Development.

[9]  Matthias Chiquet,et al.  Tenascin interferes with fibronectin action , 1988, Cell.

[10]  G M Edelman,et al.  A cDNA clone for cytotactin contains sequences similar to epidermal growth factor-like repeats and segments of fibronectin and fibrinogen. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[11]  I. Thesleff,et al.  Tenascin is associated with chondrogenic and osteogenic differentiation in vivo and promotes chondrogenesis in vitro , 1987, The Journal of cell biology.

[12]  I. Thesleff,et al.  Changes in the distribution of tenascin during tooth development. , 1987, Development.

[13]  H. Erickson,et al.  Hexabrachion proteins in embryonic chicken tissues and human tumors , 1987, The Journal of cell biology.

[14]  R. Chiquet‐Ehrismann,et al.  Epithelial-mesenchymal interactions in the developing kidney lead to expression of tenascin in the mesenchyme , 1987, The Journal of cell biology.

[15]  R. Chiquet‐Ehrismann,et al.  Tenascin: an extracellular matrix protein involved in tissue interactions during fetal development and oncogenesis , 1986, Cell.

[16]  A. Guillouzo,et al.  Cell types involved in collagen and fibronectin production in normal and fibrotic human liver , 1986, Hepatology.

[17]  G. Edelman,et al.  Cytotactin, an extracellular matrix protein of neural and non-neural tissues that mediates glia-neuron interaction. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[18]  R. Timpl,et al.  The J1 glycoprotein—a novel nervous system cell adhesion molecule of the L2/HNK-1 family , 1985, Nature.

[19]  L. Liotta,et al.  Basement Membrane Production by Hepatocytes in Chronic Liver Disease , 1984, Hepatology.

[20]  H. Erickson,et al.  A six-armed oligomer isolated from cell surface fibronectin preparations , 1984, Nature.

[21]  M. Chiquet,et al.  Chick myotendinous antigen. I. A monoclonal antibody as a marker for tendon and muscle morphogenesis , 1984, The Journal of cell biology.

[22]  D. Bigner,et al.  Human glioma-mesenchymal extracellular matrix antigen defined by monoclonal antibody. , 1983, Cancer research.

[23]  W. Carter,et al.  A new cell surface, detergent-insoluble glycoprotein matrix of human and hamster fibroblasts. The role of disulfide bonds in stabilization of the matrix. , 1981, The Journal of biological chemistry.

[24]  B. Gusterson,et al.  The distribution of immuno-reactive tenascin in the epithelial-mesenchymal junctional areas of benign and malignant squamous epithelia , 1990, Virchows Archiv. B, Cell pathology including molecular pathology.

[25]  K. Hata.  Characteristics of the sinusoidal wall in human hepatocellular carcinoma. Ultrastructural and immunohistochemical study. , 1990 .

[26]  G. Biagini,et al.  Liver fibrosis and extracellular matrix. , 1989, Journal of hepatology.

[27]  P. Nakane,et al.  Studies on translocation of immunoglobulins across intestinal epithelium. II. Immunoelectron-microscopic localization of immunoglobulins and secretory component in human intestinal mucosa. , 1976, Gastroenterology.