Highly sensitive olfactory biosensors for the detection of volatile organic compounds by surface plasmon resonance imaging.

Nowadays, monitoring of volatile organic compounds (VOCs) is very important in various domains. In this work, we aimed to develop sensitive olfactory biosensors using odorant binding proteins (OBPs) as sensing materials. Three rat OBP3 derivatives with customized binding properties were designed and immobilized on the same chip for the detection of VOCs in solution by surface plasmon resonance imaging (SPRi). We demonstrated that the proteins kept their binding properties after the immobilization under optimized conditions. The obtained olfactory biosensors exhibited very low limits of detection in both concentration (200 pM of β-ionone) and in molecular weight of VOCs (100 g/mol for hexanal). Such a performance obtained with SPRi in solution is especially remarkable. We hypothesized that the binding of VOCs to the active sites of OBPs induced a local conformational change in the proteins. This change would give rise to a variation of refractive index, to which SPRi is extremely sensitive. In addition, the olfactory biosensors showed a high selectivity especially at relatively low VOC concentrations. With optimized regeneration procedures, they also showed very good repeatability not only from measurement to measurement but also from chip to chip with a lifespan up to almost two months. These olfactory biosensors are particularly interesting for trace detection of VOCs in solution.

[1]  Qingjun Liu,et al.  Extracellular potentials recording in intact olfactory epithelium by microelectrode array for a bioelectronic nose. , 2010, Biosensors & bioelectronics.

[2]  David Perahia,et al.  Odorant Binding and Conformational Dynamics in the Odorant-binding Protein* , 2006, Journal of Biological Chemistry.

[3]  T. Livache,et al.  SPR imaging based electronic tongue via landscape images for complex mixture analysis. , 2014, Talanta.

[4]  S. d'Auria,et al.  Odorant detection via Solidly Mounted Resonator biosensor , 2012, 2012 IEEE International Ultrasonics Symposium.

[5]  N. Jaffrezic‐Renault,et al.  Study of Langmuir and Langmuir-Blodgett films of odorant-binding protein/amphiphile for odorant biosensors. , 2005, Langmuir : the ACS journal of surfaces and colloids.

[6]  E. Peyrin,et al.  Linear Chain Formation of Split-Aptamer Dimers on Surfaces Triggered by Adenosine. , 2017, Langmuir : the ACS journal of surfaces and colloids.

[7]  Kea-Tiong Tang,et al.  Towards a Chemiresistive Sensor-Integrated Electronic Nose: A Review , 2013, Sensors.

[8]  T. Livache,et al.  Electronic tongue generating continuous recognition patterns for protein analysis. , 2014, Journal of visualized experiments : JoVE.

[9]  J. Homola.  Surface plasmon resonance sensors for detection of chemical and biological species. , 2008, Chemical reviews.

[10]  Tomasz Wasilewski,et al.  Bioelectronic nose: Current status and perspectives. , 2017, Biosensors & bioelectronics.

[11]  Gibum Kim,et al.  SPR microscopy and its applications to high-throughput analyses of biomolecular binding events and their kinetics. , 2007, Biomaterials.

[12]  Chunsheng Wu,et al.  Recent advances in olfactory receptor-based biosensors. , 2013, Biosensors & bioelectronics.

[13]  F. Shimizu,et al.  Surface plasmon resonance biosensor for enzymatic detection of small analytes , 2017, Nanotechnology.

[14]  C. Nespoulous,et al.  A single lysyl residue defines the binding specificity of a human odorant‐binding protein for aldehydes , 2006, FEBS letters.

[15]  Rita Meunier-Prest,et al.  Electrochemical detection of the 2-isobutyl-3-methoxypyrazine model odorant based on odorant-binding proteins: the proof of concept. , 2015, Bioelectrochemistry.

[16]  Jing Jiang,et al.  Nanoplasmonic monitoring of odorants binding to olfactory proteins from honeybee as biosensor for chemical detection , 2015 .

[17]  M. Benetti,et al.  Detection of odorant molecules via surface acoustic wave biosensor array based on odorant-binding proteins. , 2013, Biosensors & bioelectronics.

[18]  Qingjun Liu,et al.  Impedance spectroscopy analysis of human odorant binding proteins immobilized on nanopore arrays for biochemical detection. , 2016, Biosensors & bioelectronics.

[19]  Mohammad Yusuf Mulla,et al.  Capacitance-modulated transistor detects odorant binding protein chiral interactions , 2015, Nature Communications.

[20]  Kyoungsook Park,et al.  Detection of Bax protein conformational change using a surface plasmon resonance imaging-based antibody chip. , 2005, Biochemical and biophysical research communications.

[21]  Krishna C. Persaud,et al.  Structure and biotechnological applications of odorant-binding proteins , 2013, Applied Microbiology and Biotechnology.

[22]  Changjun Hou,et al.  Development of Cell-Based Olfactory Biosensors , 2012 .

[23]  Qingjun Liu,et al.  Olfactory biosensor based on odorant-binding proteins of Bactrocera dorsalis with electrochemical impedance sensing for pest management , 2017 .

[24]  Tai Hyun Park,et al.  Cell-based microfluidic platform for mimicking human olfactory system. , 2015, Biosensors & bioelectronics.

[25]  T. Livache,et al.  Real time monitoring of thrombin interactions with its aptamers: insights into the sandwich complex formation. , 2013, Biosensors & bioelectronics.

[26]  Josep Samitier,et al.  Advances in the production, immobilization, and electrical characterization of olfactory receptors for olfactory nanobiosensor development , 2006 .

[27]  Qingjun Liu,et al.  Fat taste detection with odorant-binding proteins (OBPs) on screen-printed electrodes modified by reduced graphene oxide , 2017 .

[28]  T. Livache,et al.  Continuous evolution profiles for electronic-tongue-based analysis. , 2012, Angewandte Chemie.

[29]  Xinping Huang,et al.  Label-free imaging, detection, and mass measurement of single viruses by surface plasmon resonance , 2010, Proceedings of the National Academy of Sciences.

[30]  Wojciech Kamysz,et al.  Advances in olfaction-inspired biomaterials applied to bioelectronic noses , 2018 .

[31]  J. B. Pitner,et al.  Using receptor conformational change to detect low molecular weight analytes by surface plasmon resonance. , 2001, Analytical chemistry.

[32]  P Bergonzo,et al.  Grafting odorant binding proteins on diamond bio-MEMS. , 2014, Biosensors & bioelectronics.

[33]  Stefan Schütz,et al.  Electronic Olfactory Sensor Based on A. mellifera Odorant‐Binding Protein 14 on a Reduced Graphene Oxide Field‐Effect Transistor , 2015, Angewandte Chemie.

[34]  H. Sota,et al.  Detection of conformational changes in an immobilized protein using surface plasmon resonance. , 1998, Analytical chemistry.

[35]  L. Briand,et al.  Odorant‐Binding Proteins and Xenobiotic Metabolizing Enzymes: Implications in Olfactory Perireceptor Events , 2013, Anatomical record.

[36]  H. Breer,et al.  Odorants of different chemical classes interact with distinct odorant binding protein subtypes. , 2002, Chemical senses.

[37]  Stefan Schütz,et al.  Fine discrimination of volatile compounds by graphene-immobilized odorant-binding proteins , 2018 .

[38]  C. Nespoulous,et al.  Odorant binding and conformational changes of a rat odorant-binding protein. , 2004, Chemical senses.

[39]  Dynamics of conformational Ca2+-switches in signaling networks detected by a planar plasmonic device. , 2012, Analytical chemistry.

[40]  Josep Samitier,et al.  A novel detection strategy for odorant molecules based on controlled bioengineering of rat olfactory receptor I7. , 2007, Biosensors & bioelectronics.