Chasing lipids in health and diseases by coherent anti-Stokes Raman scattering microscopy.

The integration of near IR picosecond pulse excitation, collinear beam geometry, epi-detection, and laser-scanning has produced a coherent anti-Stokes Raman scattering (CARS) microscope with a detection sensitivity of 10(5) vibrational oscillators, sub-micron 3D resolution, and video-rate acquisition speed. The incorporation of spectral detection and other imaging modalities has added versatility to the CARS microscope. These advances allowed sensitive interrogation of biological samples, particularly lipids that have a high density of CH(2) groups. With initial applications to membrane domains, lipid bodies, demyelinating diseases, obesity, and cardiovascular diseases, CARS microscopy is poised to become a powerful bio-imaging tool with the availability of a multifunctional, affordable, easy-to-operate CARS microscope, and the development of CARS endoscopy for in vivo diagnosis.

[1]  T. T. Le,et al.  Label-free Imaging of Arterial Cells and Extracellular Matrix Using a Multimodal CARS Microscope. , 2008, Optics communications.

[2]  Catharina de Lange Davies,et al.  Characterization of vulnerable plaques by multiphoton microscopy. , 2007, Journal of biomedical optics.

[3]  Juleon M. Schins,et al.  Imaging the Thermodynamic State of Lipid Membranes with Multiplex CARS Microscopy , 2002 .

[4]  Riyi Shi,et al.  Coherent anti-stokes Raman scattering imaging of axonal myelin in live spinal tissues. , 2005, Biophysical journal.

[5]  Mischa Bonn,et al.  Quantitative multiplex CARS spectroscopy in congested spectral regions. , 2006, The journal of physical chemistry. B.

[6]  Eric O Potma,et al.  Direct visualization of lipid phase segregation in single lipid bilayers with coherent anti-Stokes Raman scattering microscopy. , 2005, ChemPhysChem.

[7]  Andreas Volkmer,et al.  Theoretical and experimental characterization of coherent anti-Stokes Raman scattering microscopy , 2002 .

[8]  Andreas Volkmer,et al.  An Epi-Detected Coherent Anti-Stokes Raman Scattering (E-CARS) Microscope with High Spectral Resolution and High Sensitivity , 2001 .

[9]  Ji-Xin Cheng,et al.  Coherent anti-Stokes Raman scattering imaging with a laser source delivered by a photonic crystal fiber. , 2006, Optics letters.

[10]  Tak W. Kee,et al.  Simple approach to one-laser, broadband coherent anti-Stokes Raman scattering microscopy. , 2004, Optics letters.

[11]  Nanosecond microscopy with spectroscopic resolution , 2005, physics/0512226.

[12]  Ji‐Xin Cheng,et al.  Imaging receptor-mediated endocytosis with a polymeric nanoparticle-based coherent anti-stokes Raman scattering probe. , 2007, The journal of physical chemistry. B.

[13]  B. Bergk,et al.  Magnetic-field- and temperature-dependent Fermi surface of CeBiPt , 2006 .

[14]  Ji-Xin Cheng,et al.  Coherent Anti-Stokes Raman Scattering Microscopy , 2007, 2008 Conference on Lasers and Electro-Optics and 2008 Conference on Quantum Electronics and Laser Science.

[15]  Li Li,et al.  Coexisting stripe- and patch-shaped domains in giant unilamellar vesicles. , 2006, Biochemistry.

[16]  X. Xie,et al.  Nonperturbative chemical imaging of organelle transport in living cells with coherent anti-stokes Raman scattering microscopy. , 2006, Biophysical journal.

[17]  Yaochun Shen,et al.  Introduction to Nonlinear Laser Spectroscopy , 1984 .

[18]  T. B. Huff,et al.  In vivo coherent anti‐Stokes Raman scattering imaging of sciatic nerve tissue , 2007, Journal of microscopy.

[19]  Heung-Shik Park,et al.  Three-dimensional imaging of chemical bond orientation in liquid crystals by coherent anti- Stokes Raman scattering microscopy. , 2007, Optics express.

[20]  Stephen R. Leone,et al.  Chemical Imaging of Photoresists with Coherent Anti-Stokes Raman Scattering (CARS) Microscopy , 2004 .

[21]  Ji-Xin Cheng,et al.  Label-free molecular imaging of atherosclerotic lesions using multimodal nonlinear optical microscopy. , 2007, Journal of biomedical optics.

[22]  X. Xie,et al.  Coherent Anti-Stokes Raman Scattering Microscopy: Instrumentation, Theory, and Applications , 2004 .

[23]  Kinam Park,et al.  In situ visualization of paclitaxel distribution and release by coherent anti-Stokes Raman scattering microscopy. , 2006, Analytical chemistry.

[24]  M D Duncan,et al.  Scanning coherent anti-Stokes Raman microscope. , 1982, Optics letters.

[25]  Monika Ritsch-Marte,et al.  Selective imaging of saturated and unsaturated lipids by wide-field CARS-microscopy. , 2008, Optics express.

[26]  H. Hamaguchi,et al.  Vibrationally resonant imaging of a single living cell by supercontinuum-based multiplex coherent anti-Stokes Raman scattering microspectroscopy. , 2005, Optics express.

[27]  X. Xie,et al.  Living Cells as Test Tubes , 2006, Science.

[28]  W. R. Wiley,et al.  Three-Dimensional Vibrational Imaging by Coherent Anti-Stokes Raman Scattering , 1999 .

[29]  Stephen T. C. Wong,et al.  Chemically-selective imaging of brain structures with CARS microscopy. , 2007, Optics express.

[30]  Conor L Evans,et al.  Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopy. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[31]  P. Libby,et al.  Stabilization of atherosclerotic plaques: New mechanisms and clinical targets , 2002, Nature Medicine.

[32]  Eric O. Potma,et al.  Detection of single lipid bilayers with coherent anti‐Stokes Raman scattering (CARS) microscopy , 2003 .

[33]  Annika Enejder,et al.  Monitoring of lipid storage in Caenorhabditis elegans using coherent anti-Stokes Raman scattering (CARS) microscopy , 2007, Proceedings of the National Academy of Sciences.

[34]  Label-free coherent anti-stokes Raman scattering imaging of coexisting lipid domains in single bilayers. , 2008, The journal of physical chemistry. B.

[35]  D. Brasaemle,et al.  A proposed model of fat packaging by exchangeable lipid droplet proteins , 2006, FEBS letters.

[36]  Riyi Shi,et al.  Characterization of photodamage in coherent anti-Stokes Raman scattering microscopy. , 2006, Optics express.

[37]  P. Molinoff,et al.  Basic Neurochemistry: Molecular, Cellular and Medical Aspects , 1989 .

[38]  Mischa Bonn,et al.  Direct extraction of Raman line-shapes from congested CARS spectra. , 2006, Optics express.

[39]  R. Shi,et al.  Coherent anti‐stokes Raman scattering imaging of myelin degradation reveals a calcium‐dependent pathway in lyso‐PtdCho‐induced demyelination , 2007, Journal of neuroscience research.

[40]  Gengfeng Zheng,et al.  Laser-scanning coherent anti-Stokes Raman scattering microscopy and applications to cell biology. , 2002, Biophysical journal.

[41]  X. Xie,et al.  Vibrational imaging of lipid droplets in live fibroblast cells with coherent anti-Stokes Raman scattering microscopy Published, JLR Papers in Press, August 16, 2003. DOI 10.1194/jlr.D300022-JLR200 , 2003, Journal of Lipid Research.

[42]  H. Hamaguchi,et al.  Ultrabroadband (>2500cm−1) multiplex coherent anti-Stokes Raman scattering microspectroscopy using a supercontinuum generated from a photonic crystal fiber , 2005 .

[43]  S. Kawata,et al.  Tip-enhanced coherent anti-stokes Raman scattering for vibrational nanoimaging. , 2004, Physical review letters.

[44]  Vladislav Yakovlev,et al.  Enhancing red-shifted white-light continuum generation in optical fibers for applications in nonlinear Raman microscopy. , 2005, Optics express.

[45]  S. Waxman.  The Axon : structure, function, and pathophysiology , 1995 .

[46]  Andreas Volkmer,et al.  Vibrational Imaging with High Sensitivity via Epidetected Coherent Anti-Stokes Raman Scattering Microscopy , 2001 .

[47]  Andreas Volkmer,et al.  Multiplex Coherent Anti-Stokes Raman Scattering Microspectroscopy and Study of Lipid Vesicles , 2002 .

[48]  M. Edidin.  The state of lipid rafts: from model membranes to cells. , 2003, Annual review of biophysics and biomolecular structure.

[49]  E. Kang,et al.  Paclitaxel distribution in poly(ethylene glycol)/poly(lactide-co-glycolic acid) blends and its release visualized by coherent anti-Stokes Raman scattering microscopy. , 2007, Journal of controlled release : official journal of the Controlled Release Society.

[50]  R. Shi,et al.  Second harmonic and sum frequency generation imaging of fibrous astroglial filaments in ex vivo spinal tissues. , 2007, Biophysical journal.

[51]  Juleon M. Schins,et al.  Direct measurement of chain order in single phospholipid mono- and bilayers with multiplex CARS , 2004 .

[52]  Ji‐Xin Cheng,et al.  Increasing the imaging depth of coherent anti-Stokes Raman scattering microscopy with a miniature microscope objective. , 2007, Optics letters.

[53]  Monika Ritsch-Marte,et al.  CARS microscopy in a wide‐field geometry with nanosecond pulses , 2006 .

[54]  Ji-Xin Cheng,et al.  Nonlinear Optical Imaging to Evaluate the Impact of Obesity on Mammary Gland and Tumor Stroma , 2007, Molecular imaging.

[55]  M. Welte.  Proteins under new management: lipid droplets deliver. , 2007, Trends in cell biology.

[56]  Feruz Ganikhanov,et al.  Broadly tunable dual-wavelength light source for coherent anti-Stokes Raman scattering microscopy. , 2006, Optics letters.

[57]  K. Peiponen,et al.  Phase retrieval in nonlinear optical spectroscopy by the maximum-entropy method: an application to the |χ (3) | spectra of polysilane , 1996 .

[58]  J. Thøgersen,et al.  Coherent anti-Stokes Raman scattering microscopy with a photonic crystal fiber based light source , 2003, Conference on Lasers and Electro-Optics, 2003. CLEO '03..

[59]  Li Li,et al.  Quantitative coherent anti-Stokes Raman scattering imaging of lipid distribution in coexisting domains. , 2005, Biophysical journal.

[60]  P. Bozza,et al.  Leukocyte lipid bodies regulation and function: contribution to allergy and host defense. , 2007, Pharmacology & therapeutics.

[61]  X. Xie,et al.  Towards CARS Endoscopy. , 2006, Optics express.