ANTIOXIDANT ACTIVITY AND BIOGENIC SYNTHESIS OF SELENIUM NANOPARTICLES USING THE LEAF EXTRACT OF ALOE VERA

Objective: The objective of this present study were to the biogenic synthesis of selenium nanoparticles using Aloe vera extract and check it’s antioxidant potential by ABTS, DPPH and FRAP assays. Methods: In this study we investigated the clove of Aloe vera , which is used for the synthesis of selenium nanoparticles were characterized by using UV-Visible (UV-VIS) spectrophotometer, Transmission electron microscopy (TEM), Fourier transform spectroscopy (FTIR) and Energy dispersive X-Ray spectroscopy (EDAX) and ABTS, DPPH and FRAP assays for checked it’s antioxidant potential. Results: The present study was carried out to synthesis of Selenium nanoparticles using extract of Aloe vera . UV-Vis Spectra at 350 nm with Aloe vera extract and observed as hollow and spherical particles in size ranging 7-48 nm which is found more stable more than two months. EDAX analysis was carried out to check the presoak of Selenium in nanoparticles. Results of EDAX, confirmed its present. TEM and SEAD represented addition evidence of formation of nanoparticles whereas SEAD indicates the particles were crystalline in nature. FT-IR analysis was carried out to identify the possible bio molecules and Aloe vera extract-metal ions interaction responsible for formation and stabilization of selenium nanoparticles. FRAP, ABTS and DPPH assay results sequester that Selenium nanoparticles prepared using Aloe vera extract possess more activity than extract alone. Conclusion: The bio molecules of Aloe vera extract acted as stabilizing as well as capping agent leading to the formation of Selenium nanoparticles. Selenite has been proven to have antioxidant activity and is being used as chemoprevention agent in cancer diagnosis but same time it is toxic also. Elemental Selenium i.e. Selenium nanoparticles are less toxic form of selenium.

[1]  K. Prasad,et al.  Synthesis of water soluble CdS nanoparticles and study of their DNA damage activity , 2017 .

[2]  N. Mondal,et al.  Biosynthesis of silver nanoparticles from Aloe vera leaf extract and antifungal activity against Rhizopus sp. and Aspergillus sp. , 2015, Applied Nanoscience.

[3]  Ashish Ranjan Sharma,et al.  Biomolecule-Mediated Synthesis of Selenium Nanoparticles using Dried Vitis vinifera (Raisin) Extract , 2014, Molecules.

[4]  K. Prasad,et al.  Biosynthesis of Se nanoparticles and its effect on UV-induced DNA damage. , 2013, Colloids and surfaces. B, Biointerfaces.

[5]  K. Sampath,et al.  Green synthesis and characterization of selenium nanoparticles and its augmented cytotoxicity with doxorubicin on cancer cells , 2013, Bioprocess and Biosystems Engineering.

[6]  M. Faramarzi,et al.  Acute and subacute toxicity of novel biogenic selenium nanoparticles in mice , 2013, Pharmaceutical biology.

[7]  Siavash Iravani,et al.  Green synthesis of metal nanoparticles using plants , 2011 .

[8]  S. Ghosh,et al.  Signaling gene cascade in silver nanoparticle induced apoptosis. , 2010, Colloids and surfaces. B, Biointerfaces.

[9]  Mojtaba Shakibaie,et al.  Biosynthesis of selenium nanoparticles using Klebsiella pneumoniae and their recovery by a simple sterilization process , 2010, Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology].

[10]  T. C. Prathna,et al.  Biomimetic Synthesis of Nanoparticles: Science, Technology & Applicability , 2010 .

[11]  Dejian Huang,et al.  The chemistry behind antioxidant capacity assays. , 2005, Journal of agricultural and food chemistry.

[12]  F. Tomás-Barberán,et al.  Antioxidant capacities, phenolic compounds, carotenoids, and vitamin C contents of nectarine, peach, and plum cultivars from California. , 2002, Journal of agricultural and food chemistry.

[13]  R. D. Pinto,et al.  Synthesis of nanocrystalline material by sputtering and laser ablation at low temperatures , 2001 .

[14]  M. B. Arnao,et al.  The hydrophilic and lipophilic contribution to total antioxidant activity , 2001 .

[15]  Christopher B. Murray,et al.  Synthesis and Characterization of Monodisperse Nanocrystals and Close-Packed Nanocrystal Assemblies , 2000 .

[16]  J J Strain,et al.  The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. , 1996, Analytical biochemistry.

[17]  Deepa..  Biogenic Synthesis and Characterization of Selenium Nanoparticles Using the Flower of Bougainvillea spectabilis Willd , 2015 .

[18]  J. Abraham,et al.  Biosynthesis of silver and selenium nanoparticles by Bacillus sp. JAPSK2 and evaluation of antimicrobial activity , 2014 .

[19]  Devaraju Rakshith,et al.  Plants as Green Source towards Synthesis of Nanoparticles , 2013 .

[20]  N. Rajurkar,et al.  Evaluation of phytochemicals , antioxidant activity and elemental content of Adiantum capillus veneris leaves , 2012 .

[21]  B. Joseph,et al.  PHARMACOGNOSTIC AND PHYTOCHEMICAL PROPERTIES OF ALOE VERA LINN -AN OVERVIEW , 2010 .

[22]  T. C. Prathna,et al.  Biomimetic Synthesis of Nanoparticles: Science, Technology , 2010 .

[23]  Priyabrata Mukherjee,et al.  The use of microorganisms for the formation of metal nanoparticles and their application , 2005, Applied Microbiology and Biotechnology.

[24]  C. Berset,et al.  Use of a Free Radical Method to Evaluate Antioxidant Activity , 1995 .