Method optimization by solid-phase microextraction in combination with gas chromatography with mass spectrometry for analysis of beer volatile fraction.

A simple and sensitive method for the analysis of beer volatile compounds was optimised using headspace solid-phase microextraction (SPME) and gas chromatography with mass detection. Headspace SPME using a 75 microm Carboxen-polydimethylsiloxane (CAR-PDMS) fiber provided effective sample enrichment and enabled extraction of a wide variety of compounds. The reproducibility depended on the compounds, with a mean value of 1.4% for alcohols, 3.3% for ethers, 6.7% for aldehydes, 3.4% for acids, 1.7% for aromatic compounds, 2.4% for esters, 7.4% for hydrocarbons, 1.8% for alicyclic compounds, and 3.4% for heterocyclic compounds. The optimised methodology can be used to compare volatile profile from different types of beers and eventually to study the evolution of a particular beer during aging.

[1]  K. Verstrepen,et al.  Evolution of chemical and sensory properties during aging of top-fermented beer. , 2003, Journal of agricultural and food chemistry.

[2]  P. J. Almeida,et al.  Simultaneous determination of E-2-nonenal and beta-damascenone in beer by reversed-phase liquid chromatography with UV detection. , 2004, Journal of chromatography. A.

[3]  G. Aerts,et al.  The Oxygenated Sesquiterpenoid Fraction of Hops in Relation to the Spicy Hop Character of Beer , 2002 .

[4]  Henryk H. Jeleń,et al.  Solid-Phase Microextraction for the Analysis of Some Alcohols and Esters in Beer: Comparison with Static Headspace Method , 1998 .

[5]  P. Veselý,et al.  Analysis of aldehydes in beer using solid-phase microextraction with on-fiber derivatization and gas chromatography/mass spectrometry. , 2003, Journal of agricultural and food chemistry.

[6]  L. Trugo,et al.  Discrimination of different hop varieties using headspace gas chromatographic data , 2003 .

[7]  I. Ferreira,et al.  Solid-phase microextraction in combination with GC/MS for quantification of the major volatile free fatty acids in ewe cheese. , 2002, Analytical chemistry.

[8]  J. Pawliszyn,et al.  Solid phase microextraction with thermal desorption using fused silica optical fibers , 1990 .

[9]  R. Smith,et al.  Determination of sulphur compounds in beer using headspace solid-phase microextraction and gas chromatographic analysis with pulsed flame photometric detection. , 2000, Journal of chromatography. A.

[10]  L. Guido,et al.  The impact of the physiological condition of the pitching yeast on beer flavour stability: an industrial approach. , 2004 .

[11]  Guy Derdelinckx,et al.  The chemistry of beer aging – a critical review , 2006 .

[12]  L Gijs,et al.  3-methylthiopropionaldehyde as precursor of dimethyl trisulfide in aged beers. , 2000, Journal of agricultural and food chemistry.

[13]  K. Siebert,et al.  Quantitative structure-activity relationship modeling of alcohol, ester, aldehyde, and ketone flavor thresholds in beer from molecular features. , 2004, Journal of agricultural and food chemistry.

[14]  Z. Zeng,et al.  Preparation of novel solid-phase microextraction fibers by sol-gel technology for headspace solid-phase microextraction-gas chromatographic analysis of aroma compounds in beer. , 2005, Journal of chromatography. A.

[15]  Janusz Pawliszyn,et al.  Solid phase microextraction : theory and practice , 1997 .

[16]  T. Shibamoto,et al.  Antioxidative activity of volatile chemicals extracted from beer. , 2001, Journal of agricultural and food chemistry.

[17]  T. Kishimoto,et al.  Analysis of hop-derived terpenoids in beer and evaluation of their behavior using the stir bar-sorptive extraction method with GC-MS. , 2005, Journal of agricultural and food chemistry.

[18]  I. Ferreira,et al.  Solid-phase microextraction of volatile compounds in "Terrincho" ewe cheese. Comparison of different fibers. , 2003, Journal of chromatography. A.

[19]  P. Zöllner,et al.  Determination of zearalenone and its metabolites α- and β-zearalenol in beer samples by high-performance liquid chromatography-tandem mass spectrometry. , 2000 .

[20]  Janusz Pawliszyn,et al.  Headspace solid-phase microextraction , 1993 .

[21]  K. Verstrepen,et al.  Influence of the brewing process on furfuryl ethyl ether formation during beer aging. , 2004, Journal of agricultural and food chemistry.

[22]  E. Ibáñez,et al.  Solid‐Phase Microextraction (SPME) of Pyrazines in Model Reaction Systems , 1996 .

[23]  R. A. Murray.  Limitations to the use of solid-phase microextraction for quantitation of mixtures of volatile organic sulfur compounds. , 2001, Analytical chemistry.