Experimental Technique in Protein Spectroscopy

It is difficult to formulate any scientific problem without analyzing procedures and methods applied for its solution. Yet, the introduction of a chapter on experimental technique into this scientific monograph may detract from its style, disturb its harmony. And a book on protein spectroscopy would be incomplete without an analysis of experimental techniques for proteins, i.e., methods applied to gain the scientific knowledge discussed in previous chapters. It does not appear essential to discuss the design of spectroscopic instruments, but rather the requirements imposed upon their important parameters by experimentation with protein spectroscopy. It is, therefore, necessary though in brief, to analyze the problems of calibration and sources of errors and artifacts. Protein preparations often have a high level of turbidity, considerable overlapping of spectral components, and other peculiarities which require special approaches. A high methodical level is necessary in fluorescent spectroscopy, especially in spectroscopy with time resolution.