Photoperiod influences endogenous indoleamines in cultured green alga Dunaliella bardawil.

Effect of light intensity and photoperiod on growth, indoleamines and carotenoid production was studied in unicellular green algae D. bardawil. Maximum biomass and carotenoid contents were found when cultures were grown in light (intensity of 2.0 Klux) at a photoperiod of 16/8h light and dark cycle. There was a profound influence of tested photoperiod conditions of light:dark viz. 8:16, 10:14, and 12:12 hr, continuous light on indoleamines (SER and MEL) production as estimated by HPLC and confirmed by mass spectral data obtained from LC-MS-ESI studies. Serotonin level increased from 908 to 1765 pg/g fresh wt with increase in light duration and melatonin level increased from 267 to 584 pg/g fresh wt during increase in dark phase. Carotenoids production was high in continuous light than other tested conditions.

[1]  Gokare A. Ravishankar,et al.  Indoleamines and calcium channels influence morphogenesis in in vitro cultures of Mimosa pudica L. , 2009, Plant signaling & behavior.

[2]  René H Wijffels,et al.  Exploring and exploiting carotenoid accumulation in Dunaliella salina for cell-factory applications. , 2008, Trends in biotechnology.

[3]  Zhi-Wei Ye,et al.  Biosynthesis and regulation of carotenoids in Dunaliella: progresses and prospects. , 2008, Biotechnology advances.

[4]  K. Kang,et al.  Characterization of tryptamine 5-hydroxylase and serotonin synthesis in rice plants , 2007, Plant Cell Reports.

[5]  Vinod Kumar,et al.  Effect of the Carotenoid-Producing Alga, Dunaliella bardawil, on CCl4-Induced Toxicity in Rats , 2007, International journal of toxicology.

[6]  Josep M. Guerrero,et al.  Melatonin is a phytochemical in olive oil , 2007 .

[7]  P. Saxena,et al.  Rapid method for accurate analysis of melatonin, serotonin and auxin in plant samples using liquid chromatography-tandem mass spectrometry. , 2006, Journal of chromatography. A.

[8]  K. Lüning,et al.  Quantification of melatonin in phototrophic organisms , 2006, Journal of pineal research.

[9]  Tetsuya Seki,et al.  Serotonin derivatives, major safflower (Carthamus tinctorius L.) seed antioxidants, inhibit low-density lipoprotein (LDL) oxidation and atherosclerosis in apolipoprotein E-deficient mice. , 2006, Journal of agricultural and food chemistry.

[10]  S. Lele,et al.  Carotenoid production from microalga, Dunaliella salina , 2005 .

[11]  R. Reiter,et al.  Melatonin in walnuts: influence on levels of melatonin and total antioxidant capacity of blood. , 2005, Nutrition.

[12]  Bingren Xiang,et al.  Fingerprint analysis of Psoralea corylifolia L. by HPLC and LC-MS. , 2005, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[13]  M. B. Arnao,et al.  Melatonin acts as a growth‐stimulating compound in some monocot species , 2005, Journal of pineal research.

[14]  Gokare A. Ravishankar,et al.  In vivo antioxidant activity of carotenoids from Dunaliella salina--a green microalga. , 2005, Life sciences.

[15]  R. Hardeland.  The presence and function of melatonin and structurally related indoleamines in a dinoflagellate, and a hypothesis on the evolutionary significance of these tryptophan metabolites in unicellulars , 1993, Experientia.

[16]  R. Hardeland,et al.  Pineal hormone melatonin oscillates also in the dinoflagellateGonyaulax polyedra , 1991, Naturwissenschaften.

[17]  C. Vílchez,et al.  UV-A mediated induction of carotenoid accumulation in Dunaliella bardawil with retention of cell viability , 2005, Applied Microbiology and Biotechnology.

[18]  R. Hardeland,et al.  The vertebrate pineal hormone melatonin is produced by the brown alga Pterygophora californica and mimics dark effects on growth rate in the light , 1996, Planta.

[19]  C. Vílchez,et al.  Carotenoids as protective response against oxidative damage in Dunaliella bardawil. , 2003, Biomolecular engineering.

[20]  Yukui Zhang,et al.  Melatonin in Chinese medicinal herbs. , 2003, Life sciences.

[21]  R. Reiter,et al.  High levels of melatonin in the seeds of edible plants: possible function in germ tissue protection. , 2000, Life sciences.

[22]  P. Saxena,et al.  Tryptophan is a precursor for melatonin and serotonin biosynthesis in in vitro regenerated St. John's wort (Hypericum perforatum L. cv. Anthos) plants , 2000, Plant Cell Reports.

[23]  S. Rani,et al.  Light sensitivity of the photoperiodic response system in higher vertebrates: wavelength and intensity effects. , 1999, Indian journal of experimental biology.

[24]  R. Hardeland.  Melatonin and 5-methoxytryptamine in non-metazoans. , 1999, Reproduction, nutrition, development.

[25]  I. Macháčková,et al.  Melatonin : Occurrence and daily rhythm in Chenopodium rubrum , 1997 .

[26]  V. Kumar.  Melatonin: a master hormone and a candidate for universal panacea. , 1996, Indian journal of experimental biology.

[27]  R. Hardeland,et al.  Photoperiodism and effects of indoleamines in a unicellular alga, Gonyaulax polyedra. , 1991, Science.

[28]  H. Leon Harter,et al.  THE PROBABILITY INTEGRALS OF THE RANGE AND OF THE STUDENTIZED RANGE. PROBABILITY INTEGRAL AND PERCENTAGE POINTS OF THE STUDENTIZED RANGE; CRITICAL VALUES FOR DUNCAN'S NEW MULTIPLE RANGE TEST , 1959 .