Preliminary Study on Biosensor-Type Time-Temperature Integrator for Intelligent Food Packaging

A glucose biosensor was utilized as a platform for the time-temperature integrator (TTI), a device for intelligent food packaging. The TTI system is composed of glucose oxidase, glucose, a pH indicator, and a three-electrode potentiostat, which produces an electrical signal as well as color development. The reaction kinetics of these response variables were analyzed under isothermal conditions. The reaction rates of the electrical current and color changes were 0.0360 ± 0.0020 (95% confidence limit), 0.0566 ± 0.0026, 0.0716 ± 0.0024, 0.1073 ± 0.0028 µA/min, and 0.0187 ± 0.0005, 0.0293 ± 0.0018, 0.0363 ± 0.0012, 0.0540 ± 0.0019 1/min, at 5, 15, 25, and 35 °C, respectively. The Arrhenius activation energy of the current reaction (Eacurrent) was 25.0 ± 1.6 kJ/mol and the Eacolor of the color reactions was 24.2 ± 0.6 kJ/mol. The similarity of these Ea shows agreement in the prediction of food qualities between the electrical signal and color development. Consequently, the function of the new time-temperature integrator system could be extended to that of a biosensor compatible with any electrical utilization equipment.

[1]  Seung-Won Jung,et al.  A feasibility study of application of laccase-based time-temperature indicator to kimchi quality control on fermentation process , 2014, Journal of the Korean Society for Applied Biological Chemistry.

[2]  David Hillier,et al.  Radio frequency identification and food retailing in the UK , 2005 .

[3]  Ann Van Loey,et al.  Intrinsic time temperature integrators for heat treatment of milk , 2002 .

[4]  Yun Xiang,et al.  Quantum-dot/aptamer-based ultrasensitive multi-analyte electrochemical biosensor. , 2006, Journal of the American Chemical Society.

[5]  R. V. Van Duyne,et al.  A nanoscale optical biosensor: sensitivity and selectivity of an approach based on the localized surface plasmon resonance spectroscopy of triangular silver nanoparticles. , 2002, Journal of the American Chemical Society.

[6]  K. Koutsoumanis,et al.  Applicability of a microbial Time Temperature Indicator (TTI) for monitoring spoilage of modified atmosphere packed minced meat. , 2009, International journal of food microbiology.

[7]  Fernanda A. R. Oliveira,et al.  Smart packaging technologies for fruits and vegetables , 2008 .

[8]  Application of mixture rule to determine arrhenius activation energy of time temperature integrator using mixture of laccase from Pleurotus ostreatus and PEGylated laccase from Trametes versicolor , 2013, Journal of the Korean Society for Applied Biological Chemistry.

[9]  Theodore P. Labuza,et al.  Predictive Microbiology for Monitoring Spoilage of Dairy Products with Time-Temperature Integrators , 1991 .

[10]  D. Burk,et al.  The Determination of Enzyme Dissociation Constants , 1934 .

[11]  Time-Temperature Indicator using Phospholipid-Phospholipase System and Application to Storage of Frozen Pork , 1994 .

[12]  D. J. Porter,et al.  7 Flavoprotein Oxidases , 1975 .

[13]  G. Kohring,et al.  Production, purification and characterization of glucose oxidase from a newly isolated strain of Penicillium pinophilum , 1997, Applied Microbiology and Biotechnology.

[14]  Thomas H. Shellhammer,et al.  Monitoring Chemical and Microbial Changes of Cottage Cheese using a Full‐history Time‐temperature Indicator , 1991 .

[15]  A. Abakarov,et al.  TIME‐TEMPERATURE INDICATOR TO MONITOR COLD CHAIN DISTRIBUTION OF FRESH SALMON ( SALMO SALAR ) , 2012 .

[16]  S. Isobe,et al.  Prediction of pathogen growth on iceberg lettuce under real temperature history during distribution from farm to table. , 2005, International journal of food microbiology.

[17]  Sun Yan,et al.  Development and characterization of a new amylase type time–temperature indicator , 2008 .

[18]  Mauro Gamberi,et al.  Traceability of food products: General framework and experimental evidence , 2007 .

[19]  G. Dryhurst,et al.  Redox Chemistry and Interfacial Behavior of Biological Molecules , 1988, Springer US.

[20]  H. Park,et al.  Adjustment of Arrhenius activation energy of laccase-based time–temperature integrator (TTI) using sodium azide , 2013 .

[21]  Y. Galagan,et al.  Fadable ink for time-temperature control of food freshness: Novel new time-temperature indicator , 2008 .

[22]  Martin M. F. Choi,et al.  Development and analytical application of an uric acid biosensor using an uricase-immobilized eggshell membrane. , 2007, Biosensors & bioelectronics.

[23]  Enzyme-electropolymer-based amperometric biosensors: an innovative platform for time-temperature integrators. , 2005 .

[24]  M. Dixon-Woods,et al.  Enzymes. 3rd ed , 1979 .

[25]  S. Jung,et al.  Application of a Prototype of Microbial Time Temperature Indicator (TTI) to the Prediction of Ground Beef Qualities during Storage , 2012 .

[26]  Petros Taoukis,et al.  Kinetic modelling of vitamin C loss in frozen green vegetables under variable storage conditions , 2003 .

[27]  Jiming Hu,et al.  Detection of hepatitis B virus by piezoelectric biosensor. , 2002, Journal of pharmaceutical and biomedical analysis.

[28]  Juming Tang,et al.  Enzyme-electropolymer-based amperometric biosensors: an innovative platform for time-temperature integrators. , 2005, Journal of agricultural and food chemistry.

[29]  R. Karlsson,et al.  Kinetic analysis of monoclonal antibody-antigen interactions with a new biosensor based analytical system. , 1991, Journal of immunological methods.

[30]  M. Hertog,et al.  Applicability of an enzymatic time temperature integrator as a quality indicator for mushrooms in the distribution chain , 2006 .

[31]  P. S. Taoukis,et al.  19 – Modelling the use of time-temperature indicators in distribution and stock rotation , 2001 .

[32]  Theofania Tsironi,et al.  Shelf life modelling of osmotically treated chilled gilthead seabream fillets , 2009 .

[33]  Carmen Pin,et al.  Modelling spoilage of fresh turbot and evaluation of a time-temperature integrator (TTI) label under fluctuating temperature. , 2008, International journal of food microbiology.

[34]  Paul Tobback,et al.  Active and intelligent food packaging: legal aspects and safety concerns , 2008 .

[35]  Min Jung Kim,et al.  Application of fuzzy reasoning to prediction of beef sirloin quality using time temperature integrators (TTIs) , 2012 .

[36]  M. Ellouze,et al.  Applicability of biological time temperature integrators as quality and safety indicators for meat products. , 2010, International journal of food microbiology.

[37]  G. Nychas,et al.  Field evaluation of the application of time temperature integrators for monitoring fish quality in the chill chain. , 2005, International journal of food microbiology.