Mucin 1 and interleukin-11 protein expression and inflammatory reactions in the intestinal mucosa of necrotizing enterocolitis children after surgery

BACKGROUND Necrotizing enterocolitis (NEC) of the newborn is a frequently occurring clinical disease in infants. The mortality rate of NEC in premature infants is as high as 50%, and the morbidity rate is on the rise. NEC has already caused serious impacts on newborn survival and poses serious threats to both children and families. AIM To investigate the expression and significance of mucin 1 (MUC1) and interleukin-11 (IL-11) in the intestinal mucosa of infants with neonatal NEC after surgery. METHODS Forty-eight postoperative intestinal mucosal specimens from children with NEC (NEC group) and twenty-two intestinal mucosal specimens from children with congenital intestinal atresia (control group) were collected in our hospital. Immunohistochemical staining and Western blot analysis were used to examine the protein expression of MUC-1 and IL-11 in the two groups. The serum levels of tumor necrosis factor-α (TNF-α) and IL-1β in the two groups were measured by enzyme-linked immunosorbent assay, and the relationship between MUC-1 and IL-11 protein expression and serum TNF-α and IL-1β levels was analyzed by the linear correlation method. RESULTS The protein expression of MUC-1 and IL-11 in the NEC group was significantly lower than that in the control group, and the difference was statistically significant (P < 0.05). The levels of serum TNF-α and IL-1β in the NEC group were significantly higher than those in the control group (P < 0.05). The protein expression of MUC-1 and IL-11 in the NEC group negatively correlated with serum TNF-α and IL-1β levels (P < 0.05). There was a significant negative correlation between the protein expression of MUC-1 and IL-11 and the levels of serum TNF-α and IL-1β in the NEC group. CONCLUSION The protein expression of MUC1 and IL-11 in the intestinal mucosa of children with NEC is significantly downregulated after surgery. This downregulation may be involved in the pathogenesis of this disease and has a certain correlation with inflammatory response factors in children with NEC.

[1]  G. Besner,et al.  Hope on the horizon: promising novel therapies for necrotizing enterocolitis , 2020, Pediatric Research.

[2]  P. Meier,et al.  Strategies to increase the use of mother’s own milk for infants at risk of necrotizing enterocolitis , 2020, Pediatric Research.

[3]  Terri Marin,et al.  Neonatal Microbiome and Its Relationship to Necrotizing Enterocolitis , 2020, The Journal of perinatal & neonatal nursing.

[4]  A. Maheshwari.  Role of Platelets in Neonatal Necrotizing Enterocolitis , 2020, Pediatric Research.

[5]  T. O'Shea,et al.  Genetic and epigenetic factors and early life inflammation as predictors of neurodevelopmental outcomes. , 2020, Seminars in fetal & neonatal medicine.

[6]  Hala M. Sakhr,et al.  Lactate and intestinal fatty acid binding protein as essential biomarkers in neonates with necrotizing enterocolitis: ultrasonographic and surgical considerations. , 2020, Pediatrics and neonatology.

[7]  G. Besner,et al.  Stem Cell Therapy for Preventing Neonatal Diseases in the 21st Century: Current Understanding and Challenges , 2019, Pediatric Research.

[8]  V. Rimoldi,et al.  Metagenomic analysis of intestinal mucosa revealed a specific eukaryotic gut virome signature in early-diagnosed inflammatory bowel disease , 2018, Gut microbes.

[9]  G. Besner,et al.  Human Breast Milk-Derived Extracellular Vesicles in the Protection Against Experimental Necrotizing Enterocolitis. , 2020, Journal of pediatric surgery.

[10]  A. Al-ezzy.  Immunopathological and Modulatory Effects of Cag A+ Genotype on Gastric Mucosa, Inflammatory Response, Pepsinogens, and Gastrin-17 Secretion in Iraqi Patients infected with H. pylori , 2018, Open access Macedonian journal of medical sciences.

[11]  I. Mahmutovic Persson,et al.  IL-1β mediates lung neutrophilia and IL-33 expression in a mouse model of viral-induced asthma exacerbation , 2018, Respiratory Research.

[12]  D. Kenny,et al.  Birth delivery method affects expression of immune genes in lung and jejunum tissue of neonatal beef calves , 2017, BMC Veterinary Research.

[13]  W. Lamers,et al.  Arginase 1 deletion in myeloid cells affects the inflammatory response in allergic asthma, but not lung mechanics, in female mice , 2017, BMC Pulmonary Medicine.

[14]  William L. Smith,et al.  The Anti-inflammatory Effect of Personalized Omega-3 Fatty Acid Dosing for Reducing Prostaglandin E2 in the Colonic Mucosa Is Attenuated in Obesity , 2017, Cancer Prevention Research.

[15]  C. Simopoulos,et al.  Immune and Inflammatory Responses of the Intestinal Mucosa following Extended Liver Radiofrequency Ablation , 2017, Gastroenterology research and practice.

[16]  C. Gianfrani,et al.  Cytokine production profile in intestinal mucosa of paediatric inflammatory bowel disease , 2017, PloS one.

[17]  G. Corner,et al.  MUC2 mucin deficiency alters inflammatory and metabolic pathways in the mouse intestinal mucosa , 2017, Oncotarget.

[18]  M. Drillich,et al.  Endometrial mRNA expression of selected pro-inflammatory factors and mucins in repeat breeder cows with and without subclinical endometritis. , 2017, Theriogenology.

[19]  E. Iżycka-Świeszewska,et al.  Indoleamine 2,3-dioxygenase and regulatory t cells in intestinal mucosa in children with inflammatory bowel disease. , 2017, Journal of biological regulators and homeostatic agents.