Antinociceptive Activity of Stephanolepis hispidus Skin Aqueous Extract Depends Partly on Opioid System Activation

Stephanolepis hispidus is one of the most common filefish species in Brazil. Its skin is traditionally used as a complementary treatment for inflammatory disorders. However, there are very few studies on chemical and pharmacological properties using the skin of this fish. This study was undertaken in order to investigate the effect of aqueous crude extract of S. hispidus skin (SAE) in different nociception models. Here, we report that intraperitoneal administration of SAE inhibited the abdominal constrictions induced by acetic acid in mice. In addition to the effect seen in the abdominal constriction model, SAE was also able to inhibit the hyperalgesia induced by carrageenan and prostaglandin E2 (PGE2) in mice. This potent antinociceptive effect was observed in the hot plate model too, but not in tail-flick test. Naloxone, an opioid receptor antagonist, was able to block the antinociceptive effect of SAE in the abdominal constriction and hot plate models. In addition, SAE did not present cytotoxic or genotoxic effect in human peripheral blood cells. Our results suggest that aqueous crude extract from S. hispidus skin has antinociceptive activity in close relationship with the partial activation of opioid receptors in the nervous system. Moreover, aqueous crude extract from S. hispidus skin does not present toxicity and is therefore endowed with the potential for pharmacological control of pain.

[1]  J. Calixto,et al.  TRPA1 antagonists as potential analgesic drugs. , 2012, Pharmacology & therapeutics.

[2]  J. Besson La complexité des aspects physiopharmacologiques de la douleur , 2012, Drugs.

[3]  J. Barbosa-Filho,et al.  Vasorelaxation, Induced by Dictyota pulchella (Dictyotaceae), a Brown Alga, Is Mediated via Inhibition of Calcium Influx in Rats , 2011, Marine drugs.

[4]  A. Giusti-Paiva,et al.  Anti-inflammatory and antinociceptive effects of the stem bark of Byrsonima intermedia A. Juss. , 2011, Journal of ethnopharmacology.

[5]  A. Kawabata Prostaglandin E2 and pain--an update. , 2011, Biological & pharmaceutical bulletin.

[6]  Peter McLoughlin,et al.  Prebiotics from Marine Macroalgae for Human and Animal Health Applications , 2010, Marine drugs.

[7]  David J Newman,et al.  The odyssey of marine pharmaceuticals: a current pipeline perspective. , 2010, Trends in pharmacological sciences.

[8]  M. Martins,et al.  Antinociceptive activity of aqueous extract of Bowdichia virgilioides in mice. , 2010, Journal of medicinal food.

[9]  N. Gamper,et al.  Understanding inflammatory pain: ion channels contributing to acute and chronic nociception , 2010, Pflügers Archiv - European Journal of Physiology.

[10]  D. Martens,et al.  Towards Commercial Production of Sponge Medicines , 2009, Marine drugs.

[11]  M. S. Alexandre-Moreira,et al.  The Antinociceptive and Anti-Inflammatory Activities of Caulerpin, a Bisindole Alkaloid Isolated from Seaweeds of the Genus Caulerpa , 2009, Marine drugs.

[12]  F. Perazzo,et al.  Genotoxicity testing of Ambelania occidentalis (Apocynaceae) leaf extract in vivo. , 2009, Genetics and molecular research : GMR.

[13]  A. L. Muccillo-Baisch,et al.  Effects of aqueous extract from Stephanolepis hispidus on blood pressure in the normal and in L-NAME-induced hypertensive rats , 2007, Online Brazilian Journal of Nursing.

[14]  J. J. Day,et al.  Fishes of the World, 4th Edition , 2006 .

[15]  A. Begossi,et al.  Healthy Fish: Medicinal and Recommended Species in the Amazon and the Atlantic Forest Coast (Brazil) , 2006 .

[16]  G. Speit,et al.  The comet assay: a sensitive genotoxicity test for the detection of DNA damage and repair. , 2006, Methods in molecular biology.

[17]  M. Martins,et al.  A novel hot-plate test sensitive to hyperalgesic stimuli and non-opioid analgesics. , 2005, Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas.

[18]  H. Castro-Faria-Neto,et al.  Antinociceptive effect of Nidularium procerum: a Bromeliaceae from the Brazilian coastal rain forest. , 2005, Phytomedicine : international journal of phytotherapy and phytopharmacology.

[19]  Xu Zi-rong,et al.  Biomedical Compounds from Marine organisms , 2004, Marine Drugs.

[20]  G. Barlow,et al.  Fishes of the world , 2004, Environmental Biology of Fishes.

[21]  D. Barros,et al.  Fish Balistes capriscus skin extract-induced relaxation in mesenteric arterial bed of rat. , 2003, Journal of ethnopharmacology.

[22]  José Lima de Figueiredo,et al.  Peixes da Zona Econômica exclusiva da região Sudeste-Sul do Brasil , 2002 .

[23]  F. Cunha,et al.  Involvement of resident macrophages and mast cells in the writhing nociceptive response induced by zymosan and acetic acid in mice. , 2000, European journal of pharmacology.

[24]  R. Kerr,et al.  Marine natural products as therapeutic agents , 1999 .

[25]  G. Speit,et al.  The contribution of cytotoxicity to DNA-effects in the single cell gel test (comet assay). , 1997, Toxicology letters.

[26]  J. Besson [The complexity of physiopharmacologic aspects of pain]. , 1997, Drugs.

[27]  M. Zakowski,et al.  Hot plate versus tail flick: evaluation of acute tolerance to continuous morphine infusion in the rat model. , 1995, Journal of pharmacological and toxicological methods.

[28]  H. Kobayashi A comparison between manual microscopic analysis and computerized image analysis in the single cell gel electrophoresis , 1995 .

[29]  B. Weichman,et al.  Pemedolac: a novel and long-acting non-narcotic analgesic. , 1989, The Journal of pharmacology and experimental therapeutics.

[30]  W. B. Scott,et al.  Atlantic fishes of Canada , 1988 .

[31]  W. Colburn,et al.  Pharmacokinetics of recombinant leukocyte A interferon following various routes and modes of administration to the dog. , 1985, Journal of interferon research.

[32]  C. B. Christensen,et al.  Plasma morphine concentrations during chronic oral administration in patients with cancer pain , 1982, PAIN.

[33]  R. Koster,et al.  ACETIC ACID FOR ANALGESIC SCREENING , 1959 .

[34]  Fred E. D'Amour,et al.  A METHOD FOR DETERMINING LOSS OF PAIN SENSATION , 1941 .