Comparison between Conventional Convective Heating and Microwave Heating: An FTIR Spectroscopy Study of the Effects of Microwave Oven Cooking of Bovine Breast Meat

The effects of microwave energy and conventional convective heating on bovine meat were studied in the mid-infrared region by FTIR spectroscopy, to highlight the differences between the two cooking methods. Samples of 100 g of bovine breast meat were cooked using three treatments: heating in a conventional electric oven at the temperature of 165°C for 16 min, heating in a microwave oven at 800 W for 95 sec, and heating in the same microwave oven at 650 W for 160 sec. Significant decreases in intensity of vibration bands of CH2 methylene group at 1921 and 1853 cm-1 and of the carbonyl band at 1742 cm-1 were observed after microwave heating with respect to heating in a conventional oven, showing that Maillard reaction occurs partially using microwave oven. Spectral analysis in the amide I region after microwave cooking at 800 W for 95 sec showed that an increase in intensity occurred in the region from 1665 to 1690 cm-1 which can be attributed to β-turns, characteristic of disorder processes in the protein. Further analysis after microwave cooking at 650 W for 160 sec evidenced major increase in intensity of β-turns content and the appearance of significant increases of β-sheet component at 1635 cm-1 and 1695 cm-1 that can be attributed to aggregated β-sheets structures.

[1]  L. Maillard,et al.  Action des acides amines sur les sucres : formation des melanoidines par voie methodique , 1912 .

[2]  J. Hodge Dehydrated Foods, Chemistry of Browning Reactions in Model Systems , 1953 .

[3]  L. Fulton,et al.  Roasting and braising beef roasts in microwave ovens. , 1983, Journal of the American Dietetic Association.

[4]  H. Mantsch,et al.  New insight into protein secondary structure from resolution-enhanced infrared spectra. , 1988, Biochimica et biophysica acta.

[5]  M. Namiki Chemistry of Maillard reactions: recent studies on the browning reaction mechanism and the development of antioxidants and mutagens. , 1988, Advances in food research.

[6]  F. R. Voort,et al.  Factors Affecting Differences in Milk Fat Test Obtained by Babcock, Rose-Gottlieb, and Infrared Methods and in Protein Test from Infrared Milk Analysis , 1988 .

[7]  H. Yoshida,et al.  Microwave Heating Effects on Relative Stabilities of Tocopherols in Oils , 1991 .

[8]  D. Michael P. Mingos,et al.  Applications of Microwave Dielectric Heating Effects to Synthetic Problems in Chemistry , 1991 .

[9]  D. Mingos,et al.  Applications of Microwave Dielectric Heating Effects to Synthetic Problems in Chemistry , 1991 .

[10]  F. R. van de Voort,et al.  Fourier transform infrared spectroscopy applied to food analysis , 1992 .

[11]  P. Griffiths,et al.  Effects of Sample Dilution and Particle Size/Morphology on Diffuse Reflection Spectra of Carbohydrate Systems in the Near- and Mid-Infrared. Part II: Durum Wheat , 1993 .

[12]  H. Yoshida,et al.  Microwave Heating Affects Composition and Oxidative Stability of Sesame (Sesamum indicum) Oil , 1994 .

[13]  Charles R. Buffler,et al.  Microwave Cooking and Processing: Engineering Fundamentals for the Food Scientist , 1995 .

[14]  H. Mantsch,et al.  The use and misuse of FTIR spectroscopy in the determination of protein structure. , 1995, Critical reviews in biochemistry and molecular biology.

[15]  M. C. Pérez-Camino,et al.  Microwave and Conventional Heating Effects on Some Physical and Chemical Parameters of Edible Fats , 1997 .

[16]  M. Dikeman,et al.  Tenderness and collagen composition of beef semitendinosus roasts cooked by conventional convective cooking and modeled, multi-stage, convective cooking. , 2000, Meat science.

[17]  R. C. Richter,et al.  Microwave-enhanced chemistry. , 2001, Analytical chemistry.

[18]  V. D. Pawar,et al.  Effect of fat/whey protein concentrate levels and cooking methods on textural characteristics of chevon patties , 2002 .

[19]  T. Ohlsson,et al.  COOKING | Domestic Use of Microwave Ovens , 2003 .

[20]  Paul Dumas,et al.  THE USE OF SYNCHROTRON INFRARED MICROSPECTROSCOPY IN BIOLOGICAL AND BIOMEDICAL INVESTIGATIONS , 2003 .

[21]  L. E. Jeremiah,et al.  Cooking influences on the palatability of roasts from the beef hip , 2003 .

[22]  B. Stuart Infrared Spectroscopy , 2004, Analytical Techniques in Forensic Science.

[23]  Manish Kumar,et al.  The storage stability and textural, physico‐chemical and sensory quality of low‐fat ground pork patties with Carrageenan as fat replacer , 2004 .

[24]  S. Tamminga,et al.  Probing Equivocal Effects of Heat Processing of Legume Seeds on Performance of Ruminants - A Review - , 2004 .

[25]  M. S. Herzallah Influence of Microwaving and Conventional Heating of Milk on Cholesterol Contents and Cholesterol Oxides Formation , 2005 .

[26]  E. Tornberg,et al.  Effects of heat on meat proteins - Implications on structure and quality of meat products. , 2005, Meat science.

[27]  A. Barth Infrared spectroscopy of proteins. , 2007, Biochimica et biophysica acta.

[28]  J. Kong,et al.  Fourier transform infrared spectroscopic analysis of protein secondary structures. , 2007, Acta biochimica et biophysica Sinica.

[29]  S. Yoo,et al.  Effect of Long Low Temperature-Short High Temperature Cooking Cycle on Physicochemical Properties of Beef , 2010 .

[30]  S. Magazù,et al.  Inspections of Mobile Phone Microwaves Effects on Proteins Secondary Structure by Means of Fourier Transform Infrared Spectroscopy , 2010 .

[31]  Electromagnetic Fields Effects on the Secondary Structure of Lysozyme and Bioprotective Effectiveness of Trehalose , 2012 .

[32]  S. Condello,et al.  Modulation of heat shock protein response in SH-SY5Y by mobile phone microwaves. , 2012, World journal of biological chemistry.