Genetic Diversity of Walnut Revealed by AFLP and RAPD Markers

AFLP and RAPD methods were used to investigate the genetic diversity of walnuts in western Sichuan plateau and Qinba mountainous regions. 35 samples were collected from 8 different regions, and 32 RAPD primers and 28 AFLP primer combinations were identified with polymorphism bands among the entire. 324 and 2155 fragments were respectively produced by RAPD and AFLP makers, and 86.1 % of RAPD bands and 57.2% of AFLP bands showed polymorphic with the size of 180~2000 bp and 50~1800 bp, respectively. The average amplified were 10.1 fragments per primer by RAPD and 76.9 fragments per pair primer by AFLP. The more polymorphic for genetic resource in Western Sichuan Plateau was observed by both RAPD and AFLP. The high number of alleles and the high expected genetic diversity detected with RAPD and AFLP markers indicate that western China has an important genetic diversity pool and abundant genetic variance of walnuts.

[1]  Wu Chun-lin,et al.  Studies on germplasm of Juglans by EST-SSR markers. , 2011 .

[2]  L. Qing-zhong.  ISSR Analysis of Genetic Diversity among Seedling Walnut (Juglans spp.)Populations , 2011 .

[3]  M. Martínez,et al.  Walnut (Juglans regia L.): genetic resources, chemistry, by-products. , 2010, Journal of the science of food and agriculture.

[4]  Yang Li-xue.  Effect of water extracts of larch on growth of Manchurian walnut seedlings , 2005, Journal of Forestry Research.

[5]  Li,et al.  Identification of AFLP Makers Linked to Early-Bearing Gene in Walnut , 2007 .

[6]  Yinong Xu,et al.  Walnut Staminate Flowers Can Be Explored as a Supplementary Plant Oil Source , 2006 .

[7]  S. Bughrara,et al.  Genetic diversity in colonial bentgrass (Agrostis capillaris L.) revealed by EcoRI-MseI and PstI-MseI AFLP markers. , 2006, Genome.

[8]  Di Hong.  Genetic Diversity Analysis of Chinese Main Potato Cultivars by RAPD and AFLP Makers , 2006 .

[9]  H. Özkan,et al.  DNA Polymorphism and Assessment of Genetic Relationships in Walnut Genotypes Based on AFLP and SAMPL Markers , 2005 .

[10]  S. Vilanova,et al.  Genetic diversity of loquat germplasm (Eriobotrya japonica (Thunb) Lindl) assessed by SSR markers. , 2005, Genome.

[11]  C. Jing.  Extraction of DNA for AFLP amplification reaction in teuder walnut leaves , 2004 .

[12]  Wang Guo-an.  Method of DNA Extraction and RAPD Reaction System in Walnut , 2003 .

[13]  B. Fornari,et al.  GENETIC LINKAGE MAPPING IN AN INTRASPECIFIC CROSS OF WALNUT (Juglans regia L.) USING MOLECULAR MARKERS , 2001 .

[14]  S. Fineschi,et al.  ISOZYME AND ORGANELLAR DNA ANALYSIS OF GENETIC DIVERSITY IN NATURAL/NATURALISED EUROPEAN AND ASIATIC WALNUT (Juglans regia L.) POPULATIONS , 2001 .

[15]  V. Storme,et al.  Improved AFLP analysis of tree species , 2000 .

[16]  L. Jiarui,et al.  A study on the genetic relationship among species in Juglans L. using RAPD markers. , 2000 .

[17]  P. Vos,et al.  AFLP: a new technique for DNA fingerprinting. , 1995, Nucleic acids research.