Specific covalent binding of a NF‐κB decoy hairpin oligonucleotide targeted to the p50 subunit and induction of apoptosis

The NF‐κB transcriptional factor regulates various functions such as immune responses, cellular growth and development, and is frequently activated in tumor cells. Thus, inhibition of NF‐κB could suppress tumor cell growth. Using a decoy synthetic hairpin‐shaped oligodeoxyribonucleotide (ODN) containing the κB site with an integrated single diphosphoryldisulfide linkage, we demonstrate its covalent binding to the p50 subunit of NF‐κB. Furthermore, this decoy ODN induces apoptosis in a lymphoblastoma cell line. Thus, such chemically modified decoys could be valuable tools for blocking nuclear factors and tumor cell growth.

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