In this study, the standard spectral ratio and the horizontal-to-vertical spectral ratio techniques are applied in order to study their effectiveness in investigating and quantifying the influence of geological conditions on strong ground motion. For this purpose, an accelerogram data set recorded at the Euroseistest array in the Mygdonia graben (lake Volvi area) near Thessaloniki, Greece, during the period April 1994 to June 1996 is used. Both experimental techniques show similar spectral ratio shapes with comparable fundamental resonant frequencies, which are well correlated with the well known geotechnical-geological conditions. Namely, the resonant frequency at the center of the valley is shifted to lower values, less than 1 Hz, while at the edge it is shifted to higher values, greater than 2 Hz. The horizontal-to-vertical spectral ratio technique is an effective method to estimate some basic characteristics of local site effects using a single accelerograph station. It reveals the fundamental resonant frequency of alluvial deposits by using only a single strong motion station, while the absolute level of the horizontal-to-vertical spectral ratio method tends to underestimate the amplification level compared to the standard spectral ratio technique.