In search of the minimal Escherichia coli genome.

Recent plans announced for the systematic cataloging of the minimal Escherichia coli gene set, the phenotypes of all mutations, the expression levels of every transcript and gene product, and the interactions of all genetic loci or their gene products point the way towards a new frontier in the biology of model organisms. Powerful tools for this endeavor are emerging, and efforts to organize the E. coli community are under way. The anticipated benefit is a functional model of the bacterial cell.

[1]  T Horiuchi,et al.  Functional genomics of Escherichia coli in Japan. , 2000, Research in microbiology.

[2]  E. Rubin,et al.  Comprehensive identification of conditionally essential genes in mycobacteria , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[3]  Ronald W. Davis,et al.  Functional profiling of the Saccharomyces cerevisiae genome , 2002, Nature.

[4]  Andrés Moya,et al.  Extreme genome reduction in Buchnera spp.: Toward the minimal genome needed for symbiotic life , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[5]  F. Blattner,et al.  Functional Genomics: Expression Analysis ofEscherichia coli Growing on Minimal and Rich Media , 1999, Journal of bacteriology.

[6]  C. Hoogland,et al.  New perspectives in the Escherichia coli proteome investigation , 2001, Proteomics.

[7]  Jun Hyoung Lee,et al.  Minimization of the Escherichia coli genome using a Tn5-targeted Cre/loxP excision system , 2002, Nature Biotechnology.

[8]  O. White,et al.  Global transposon mutagenesis and a minimal Mycoplasma genome. , 1999, Science.

[9]  J. Barbé,et al.  nrdD and nrdG genes are essential for strict anaerobic growth of Escherichia coli. , 1996, Biochemical and biophysical research communications.

[10]  B. Bochner,et al.  Phenotype microarrays for high-throughput phenotypic testing and assay of gene function. , 2001, Genome research.

[11]  K. Gerdes,et al.  The Escherichia coli relBE genes belong to a new toxin–antitoxin gene family , 1998, Molecular microbiology.

[12]  G. W. Hatfield,et al.  Global gene expression profiling in Escherichia coli K12. The effects of integration host factor. , 2000, The Journal of biological chemistry.

[13]  J. Mekalanos,et al.  TnAraOut, A transposon-based approach to identify and characterize essential bacterial genes , 2000, Nature Biotechnology.

[14]  J. Mekalanos,et al.  Systematic identification of essential genes by in vitro mariner mutagenesis. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[15]  Constance Holden.  Alliance Launched to Model E. coli , 2002, Science.

[16]  Ronald J Moore,et al.  Global analysis of the Deinococcus radiodurans proteome by using accurate mass tags , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[17]  J. Mekalanos,et al.  A genome-scale analysis for identification of genes required for growth or survival of Haemophilus influenzae , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[18]  Guy Plunkett,et al.  Engineering a reduced Escherichia coli genome. , 2002, Genome research.

[19]  Martin Rosenberg,et al.  Identification of Critical Staphylococcal Genes Using Conditional Phenotypes Generated by Antisense RNA , 2001, Science.

[20]  Howard Xu,et al.  A genome‐wide strategy for the identification of essential genes in Staphylococcus aureus , 2002, Molecular microbiology.

[21]  Fuchs Ja,et al.  A mutant of Escherichia coli defective in ribonucleosidediphosphate reductase. 2. Characterization of the enzymatic defect. , 1973 .