Urban vitality is an important indicator of urban development capacity. Streets’ metrics can depict intro-urban fabrics and physiognomy in detail, and thus street vitality affected by street metrics is a concrete manifestation of urban vitality. However, few studies have evaluated dynamic vitality or explored how it is influenced by land use. To bridge this gap, we fully evaluated street dynamic vitality and explored how to enhance the street dynamic vitality by changing the distribution and combination of land use. Specifically, we examined the street dynamic vitality and land use diversity in the main urban zone of Xining city in China using mobile communication and point of interest data, adopted optimized K-means clustering to identify street dynamic vitality types, evaluated the classification result based on vitality intensity and vitality stability, and explored the link between land use and dynamic vitality. Since vitality intensity limitations were found in describing street dynamic vitality, it was necessary to introduce vitality stability. We also found a positive correlation between the vitality intensity and land use density, there were outstanding traffic facilities in high-intensity vitality streets, and improving the abundance and uniformity of land use was beneficial to increase vitality stability. Overall, describing street vitality from a dynamic perspective can improve resource utilization efficiency and rationally plan layouts.