Diagnostic implications of mycetoma derived from Madurella pseudomycetomatis isolates from Mexico

At the dermatology service of the General Hospital of Mexico City, Mexico, two patients, father and son, with black‐grain mycetoma were seen. The grains were isolated, and the cultured fungi were identified as Madurella mycetomatis based on morphology. Using the M. mycetomatis specific PCR, amplicons of a different size than that of the M. mycetomatis type strain were obtained.

[1]  A. Verbon,et al.  Pyomelanin Secretion in Madurella mycetomatis Interferes with Spectrophotometric Endpoint Reading Using the Sensititre YeastOne alamarBlue Assay but Not with Visual Endpoint Reading , 2019, Antimicrobial Agents and Chemotherapy.

[2]  María de Guadalupe Chávez-López,et al.  Diagnosis and Management of Fungal Neglected Tropical Diseases In Community Settings—Mycetoma and Sporotrichosis , 2019, Tropical medicine and infectious disease.

[3]  A. Fahal,et al.  VNTR confirms the heterogeneity of Madurella mycetomatis and is a promising typing tool for this mycetoma causing agent. , 2018, Medical mycology.

[4]  A. Fahal,et al.  Closing the mycetoma knowledge gap. , 2018, Medical mycology.

[5]  T. Darré,et al.  Mycetoma in the Togolese: An Update from a Single-Center Experience , 2018, Mycopathologia.

[6]  S. de Hoog,et al.  Nigrograna mackinnonii, Not Trematosphaeria grisea (syn., Madurella grisea), Is the Main Agent of Black Grain Eumycetoma in Latin America , 2017, Journal of Clinical Microbiology.

[7]  E. A. Kouotou,et al.  Mycétome : apport de l’histopathologie au diagnostic chez un commerçant camerounais, et possibilité d’une contamination urbaine , 2017 .

[8]  V. Garg,et al.  Mycetoma: An Update , 2017, Indian journal of dermatology.

[9]  Nicholas H Oberlies,et al.  Fungal Identification Using Molecular Tools: A Primer for the Natural Products Research Community , 2017, Journal of natural products.

[10]  G. González,et al.  Molecular identification of unusual Mycetoma agents isolated from patients in Venezuela , 2017, Mycoses.

[11]  A. Fahal,et al.  Hand Mycetoma: The Mycetoma Research Centre Experience and Literature Review , 2016, PLoS neglected tropical diseases.

[12]  G. S. de Hoog,et al.  DNA barcoding of fungi causing infections in humans and animals. , 2016, Fungal biology.

[13]  D. Stevens,et al.  Revision of agents of black-grain eumycetoma in the order Pleosporales , 2014, Persoonia.

[14]  S. Bassiri-Jahromi.  Mycetoma in Iran: Causative Agents and Geographic Distribution , 2014, Indian journal of dermatology.

[15]  A. Bonifaz,et al.  Mycetoma: Experience of 482 Cases in a Single Center in Mexico , 2014, PLoS neglected tropical diseases.

[16]  M. Goodfellow,et al.  Merits and Pitfalls of Currently Used Diagnostic Tools in Mycetoma , 2014, PLoS neglected tropical diseases.

[17]  W. Sande.  Global burden of human mycetoma: a systematic review and meta-analysis. , 2013 .

[18]  A. Fahal,et al.  Predictors of cure, amputation and follow-up dropout among patients with mycetoma seen at the Mycetoma Research Centre, University of Khartoum, Sudan. , 2012, Transactions of the Royal Society of Tropical Medicine and Hygiene.

[19]  M. Hare,et al.  Identifying and reducing AFLP genotyping error: an example of tradeoffs when comparing population structure in broadcast spawning versus brooding oysters , 2012, Heredity.

[20]  G. S. de Hoog,et al.  New Species of Madurella, Causative Agents of Black-Grain Mycetoma , 2011, Journal of Clinical Microbiology.

[21]  E. Zijlstra,et al.  The safety and efficacy of itraconazole for the treatment of patients with eumycetoma due to Madurella mycetomatis. , 2011, Transactions of the Royal Society of Tropical Medicine and Hygiene.

[22]  A. van Belkum,et al.  In Vitro Susceptibility of Madurella mycetomatis to Posaconazole and Terbinafine , 2011, Antimicrobial Agents and Chemotherapy.

[23]  A. van Belkum,et al.  Madurella mycetomatis Is Not Susceptible to the Echinocandin Class of Antifungal Agents , 2010, Antimicrobial Agents and Chemotherapy.

[24]  F. Hao,et al.  Phenotypic and Molecular Characterization of Madurella pseudomycetomatis sp. nov., a Novel Opportunistic Fungus Possibly Causing Black-Grain Mycetoma , 2009, Journal of Clinical Microbiology.

[25]  D. Mathur,et al.  Incidence and changing pattern of mycetoma in western Rajasthan. , 2008, Indian journal of pathology & microbiology.

[26]  P. J. Donnelly,et al.  Comparison of Two Highly Discriminatory Molecular Fingerprinting Assays for Analysis of Multiple Aspergillus fumigatus Isolates from Patients with Invasive Aspergillosis , 2007, Journal of Clinical Microbiology.

[27]  A. van Belkum,et al.  In vitro susceptibility of Madurella mycetomatis, prime agent of Madura foot, to tea tree oil and artemisinin. , 2007, The Journal of antimicrobial chemotherapy.

[28]  M. Hare,et al.  A genomic scan for divergent selection in a secondary contact zone between Atlantic and Gulf of Mexico oysters, Crassostrea virginica , 2006, Molecular ecology.

[29]  A. van Belkum,et al.  Testing of the In Vitro Susceptibilities of Madurella mycetomatis to Six Antifungal Agents by Using the Sensititre System in Comparison with a Viability-Based 2,3-Bis(2-Methoxy-4-Nitro-5-Sulfophenyl)-5- [(Phenylamino)Carbonyl]-2H-Tetrazolium Hydroxide (XTT) Assay and a Modified NCCLS Method , 2005, Antimicrobial Agents and Chemotherapy.

[30]  A. van Belkum,et al.  In Vitro Susceptibilities of Madurella mycetomatis to Itraconazole and Amphotericin B Assessed by a Modified NCCLS Method and a Viability-Based 2,3-Bis(2-Methoxy-4-Nitro-5- Sulfophenyl)-5-[(Phenylamino)Carbonyl]-2H- Tetrazolium Hydroxide (XTT) Assay , 2004, Antimicrobial Agents and Chemotherapy.

[31]  A. van Belkum,et al.  Development of a Species-Specific PCR-Restriction Fragment Length Polymorphism Analysis Procedure for Identification ofMadurella mycetomatis , 1999, Journal of Clinical Microbiology.