Optical coherence tomography (OCT) reveals depth-resolved dynamics during functional brain activation

Optical intrinsic signal imaging (OISI) provides two-dimensional, depth-integrated activation maps of brain activity. Optical coherence tomography (OCT) provides depth-resolved, cross-sectional images of functional brain activation. Co-registered OCT and OISI imaging was performed simultaneously on the rat somatosensory cortex through a thinned skull during forepaw electrical stimulation. Fractional signal change measurements made by OCT revealed a functional signal that correlates well with that of the intrinsic hemodynamic signals and provides depth-resolved, layer-specific dynamics in the functional activation patterns indicating retrograde vessel dilation. OCT is a promising a new technology which provides complementary information to OISI for functional neuroimaging.

[1]  A. Dale,et al.  Coupling of Total Hemoglobin Concentration, Oxygenation, and Neural Activity in Rat Somatosensory Cortex , 2003, Neuron.

[2]  Ruikang K. Wang,et al.  Three dimensional optical angiography. , 2007, Optics express.

[3]  Brian J Bacskai,et al.  Four-dimensional multiphoton imaging of brain entry, amyloid binding, and clearance of an amyloid-β ligand in transgenic mice , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[4]  Ruikang K. Wang,et al.  Mapping of cerebro-vascular blood perfusion in mice with skin and skull intact by Optical Micro-AngioGraphy at 1.3 mum wavelength. , 2007, Optics express.

[5]  J G Fujimoto,et al.  Depth-resolved imaging of functional activation in the rat cerebral cortex using optical coherence tomography. , 2006, Optics letters.

[6]  James S. Schwaber,et al.  Scattered-Light Imaging in Vivo Tracks Fast and Slow Processes of Neurophysiological Activation , 2001, NeuroImage.

[7]  Anders M. Dale,et al.  Spatial extent of oxygen metabolism and hemodynamic changes during functional activation of the rat somatosensory cortex , 2005, NeuroImage.

[8]  K. Svoboda,et al.  Principles of Two-Photon Excitation Microscopy and Its Applications to Neuroscience , 2006, Neuron.

[9]  S. Charpak,et al.  Two-photon imaging of capillary blood flow in olfactory bulb glomeruli. , 2009, Methods in molecular biology.

[10]  David M. Rector,et al.  Spatio-temporal mapping of rat whisker barrels with fast scattered light signals , 2005, NeuroImage.

[11]  M. V. van Gemert,et al.  Noninvasive imaging of in vivo blood flow velocity using optical Doppler tomography. , 1997, Optics letters.

[12]  A. Toga,et al.  Linear and Nonlinear Relationships between Neuronal Activity, Oxygen Metabolism, and Hemodynamic Responses , 2004, Neuron.

[13]  J. Taylor,et al.  Optophysiology: depth-resolved probing of retinal physiology with functional ultrahigh-resolution optical coherence tomography. , 2006, Proceedings of the National Academy of Sciences of the United States of America.

[14]  Joseph M. Schmitt,et al.  Optical coherence tomography (OCT): a review , 1999 .

[15]  J. Duker,et al.  In vivo measurement of retinal physiology with high-speed ultrahigh-resolution optical coherence tomography. , 2006, Optics letters.

[16]  H. Rylander Iii,et al.  Detection of neural activity using phase-sensitive optical low-coherence reflectometry. , 2004, Optics express.

[17]  Anders M. Dale,et al.  Depth-resolved optical imaging and microscopy of vascular compartment dynamics during somatosensory stimulation , 2007, NeuroImage.

[18]  D. Ts'o,et al.  Functional organization of primate visual cortex revealed by high resolution optical imaging. , 1990, Science.

[19]  A. Grinvald,et al.  Vascular imprints of neuronal activity: relationships between the dynamics of cortical blood flow, oxygenation, and volume changes following sensory stimulation. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[20]  H. Seung,et al.  Noncontact measurement of nerve displacement during action potential with a dual-beam low-coherence interferometer. , 2004, Optics letters.

[21]  Uma Maheswari Rajagopalan,et al.  Functional optical coherence tomography reveals localized layer-specific activations in cat primary visual cortex in vivo. , 2007, Optics letters.

[22]  D. Kleinfeld,et al.  Fluctuations and stimulus-induced changes in blood flow observed in individual capillaries in layers 2 through 4 of rat neocortex. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[23]  R. Frostig,et al.  Optical imaging of neuronal activity. , 1988, Physiological reviews.

[24]  M. Tanifuji,et al.  Implementation of optical coherence tomography (OCT) in visualization of functional structures of cat visual cortex , 2002 .

[25]  Elizabeth M C Hillman,et al.  Optical brain imaging in vivo: techniques and applications from animal to man. , 2007, Journal of biomedical optics.

[26]  Arthur W Toga,et al.  Spatiotemporal Evolution of Functional Hemodynamic Changes and Their Relationship to Neuronal Activity , 2005, Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism.

[27]  A. Ngai,et al.  Pial arteriole dilation during somatosensory stimulation is not mediated by an increase in CSF metabolites. , 2002, American journal of physiology. Heart and circulatory physiology.

[28]  H. Kadono,et al.  Novel functional imaging technique from brain surface with optical coherence tomography enabling visualization of depth resolved functional structure in vivo , 2003, Journal of Neuroscience Methods.

[29]  D. Ts'o,et al.  Cortical functional architecture and local coupling between neuronal activity and the microcirculation revealed by in vivo high-resolution optical imaging of intrinsic signals. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[30]  B. Bouma,et al.  Improved signal-to-noise ratio in spectral-domain compared with time-domain optical coherence tomography. , 2003, Optics letters.

[31]  A. Grinvald,et al.  Optical mapping of electrical activity in rat somatosensory and visual cortex , 1985, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[32]  David A Boas,et al.  Laminar optical tomography: demonstration of millimeter-scale depth-resolved imaging in turbid media. , 2004, Optics letters.

[33]  J. George,et al.  Rapid optical coherence tomography and recording functional scattering changes from activated frog retina. , 2005, Applied optics.

[34]  J. Fujimoto,et al.  Optical Coherence Tomography , 1991 .

[35]  A. Dale,et al.  Coupling of the cortical hemodynamic response to cortical and thalamic neuronal activity. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[36]  David A Boas,et al.  Diffuse optical imaging of the whole head. , 2006, Journal of biomedical optics.

[37]  Maristela L Onozato,et al.  High-resolution optical coherence tomography imaging of the living kidney , 2008, Laboratory Investigation.

[38]  Changhuei Yang,et al.  Sensitivity advantage of swept source and Fourier domain optical coherence tomography. , 2003, Optics express.

[39]  A. Villringer,et al.  Non-invasive optical spectroscopy and imaging of human brain function , 1997, Trends in Neurosciences.

[40]  C. Iadecola,et al.  Neurovascular coupling in the normal brain and in hypertension, stroke, and Alzheimer disease. , 2006, Journal of applied physiology.

[41]  I Hartl,et al.  Ultrahigh resolution real time OCT imaging using a compact femtosecond Nd:Glass laser and nonlinear fiber. , 2003, Optics express.

[42]  Toshio Yanagida,et al.  In vivo imaging of the rat cerebral microvessels with optical coherence tomography. , 2004, Clinical hemorheology and microcirculation.

[43]  S. Boppart,et al.  Functional optical coherence tomography for detecting neural activity through scattering changes. , 2003, Optics letters.

[44]  G. Gratton,et al.  Shedding light on brain function: the event-related optical signal , 2001, Trends in Cognitive Sciences.

[45]  T. Ebner,et al.  Local and propagated vascular responses evoked by focal synaptic activity in cerebellar cortex. , 1997, Journal of neurophysiology.

[46]  A. Fercher,et al.  Performance of fourier domain vs. time domain optical coherence tomography. , 2003, Optics express.