Anti-Obesity and Antihyperlipidemic Effects of Musa cavendishii

Obesity is a major risk factor in many health problems. This study explored the anti-obesity and antihyperlipidemic effects of Musa cavendishii Lamb. leaves. Doses of 1200 mg/kg of M. cavendishii methanol extract, or 300 mg/kg of fractions, were given to C57BL/6J mice fed with a high-fat diet (HFD) for 7 weeks. The reduction in fat volume was determined using CT scan and histopathological examination of mice livers. The results showed that the n-hexane and the EtOAc fractions reduced the body fat volume to 2.0% and 2.2% respectively, compared to 20.1% in the HFD group. Also, treated groups showed almost normal liver architecture with no fat vacuoles compared to the HFD group. Moreover, the treated groups showed a reduction in the levels of some obesity-related biochemical parameters, including plasma glucose, cholesterol, and triglycerides levels. Chromatographic fractionation of the bioactive n-hexane extract afforded four known compounds viz., palmitic acid (1), 5-methyl-5-(4,8,12-trimethyl-tridecyl)-dihydro-furan-2-one (2), cycloeucalenol (3), and stigmasterol (4). These compounds were in vitro and in silico studied for their pancreatic lipase (PL) inhibition. Compound 2 showed remarkable PL inhibition (89.8%) compared to orlistat (85.0%) at 200 μM, which was in full agreement with the docking scores (-7.19 and -4.05, respectively).

[1]  S. Jang,et al.  Anti-obesity effects of a mixed extract containing Platycodon grandiflorum, Apium graveolens and green tea in high-fat-diet-induced obese mice. , 2020, Experimental and therapeutic medicine.

[2]  J. Paniagua.  Nutrition, insulin resistance and dysfunctional adipose tissue determine the different components of metabolic syndrome , 2016, World journal of diabetes.

[3]  F. A. Bar,et al.  Alpha-amylase Inhibitory Compounds from Musa cavendishii , 2016 .

[4]  Venkateswara Rao Talluri,et al.  Shape based virtual screening and molecular docking towards designing novel pancreatic lipase inhibitors , 2015, Bioinformation.

[5]  Yunqi Li,et al.  Caffeoylquinic acids competitively inhibit pancreatic lipase through binding to the catalytic triad. , 2015, International journal of biological macromolecules.

[6]  M. Teixeira,et al.  Activity of cycloartane-type triterpenes and sterols isolated from Musa paradisiaca fruit peel against Leishmania infantum chagasi. , 2014, Phytomedicine : international journal of phytotherapy and phytopharmacology.

[7]  N. T. Hoa,et al.  Cytotoxic Steroids from the Stem Barks of Pandanus tectorius , 2014 .

[8]  .. Silvaa,et al.  Activity of cycloartane-type triterpenes and sterols isolated from Musa paradisiaca fruit peel against Leishmania infantum chagasi , 2014 .

[9]  Soonkyu Chung,et al.  Muscadine grape (Vitis rotundifolia) and wine phytochemicals prevented obesity-associated metabolic complications in C57BL/6J mice. , 2012, Journal of agricultural and food chemistry.

[10]  Ute Roessner,et al.  A Historical Overview of Natural Products in Drug Discovery , 2012, Metabolites.

[11]  Shengting Li,et al.  Segmentation and measurement of fat volumes in murine obesity models using X-ray computed tomography. , 2012, Journal of visualized experiments : JoVE.

[12]  J. Kang,et al.  Anti-Obesity Drugs: A Review about Their Effects and Safety , 2012, Diabetes & metabolism journal.

[13]  F. Milagro,et al.  Natural Inhibitors of Pancreatic Lipase as New Players in Obesity Treatment , 2011, Planta medica.

[14]  B. Popkin.  What can public health nutritionists do to curb the epidemic of nutrition-related noncommunicable disease? , 2009, Nutrition reviews.

[15]  R. Goel,et al.  Evaluation of wound healing activity of extracts of plantain banana (Musa sapientum var. paradisiaca) in rats. , 2009, Indian journal of experimental biology.

[16]  L. Pramod,et al.  To study analgesic activity of stem of Musa sapientum Linn. , 2009 .

[17]  N. Vijayalakshmi,et al.  Antioxidant activity of banana flavonoids. , 2008, Fitoterapia.

[18]  N. Azas,et al.  Antimalarial activity of crude extracts from nine African medicinal plants. , 2008, Journal of ethnopharmacology.

[19]  D. Ghosh,et al.  Antihyperglycemic effects of separate and composite extract of root of Musa paradisiaca and leaf of Coccinia indica in streptozotocin-induced diabetic male albino rat. , 2008, African journal of traditional, complementary, and alternative medicines : AJTCAM.

[20]  C. Ragasa,et al.  A triterpene from Musa errans , 2007 .

[21]  D. Ghosh,et al.  Comparative Study on Antihyperglycemic and Antihyperlipidemic Effects of Separate and Composite Extract of Seed of Eugenia Jambolana and Root of Musa Paradisiaca in Streptozotocin-Induced Diabetic Male Albino Rat , 2006 .

[22]  T. An,et al.  Diterpene derivative and chromone from Hypericum Perforatum , 2004, Natural product research.

[23]  J. Ojewole,et al.  Hypoglycemic effect of methanolic extract of Musa paradisiaca (Musaceae) green fruits in normal and diabetic mice. , 2003, Methods and findings in experimental and clinical pharmacology.

[24]  R. Goel,et al.  Role of gastric antioxidant and anti-Helicobactor pylori activities in antiulcerogenic activity of plantain banana (Musa sapientum var. paradisiaca). , 2001, Indian journal of experimental biology.

[25]  R. Wrolstad,et al.  Anthocyanins from banana bracts (Musa X paradisiaca) as potential food colorants , 2001 .

[26]  R. Kasai,et al.  A Hemiterpenoid Glucoside from Musa paradisiaca , 2000 .

[27]  D. Lewis,et al.  A natural flavonoid present in unripe plantain banana pulp (Musa sapientum L. var. paradisiaca) protects the gastric mucosa from aspirin-induced erosions. , 1999, Journal of ethnopharmacology.

[28]  T. Akihisa,et al.  Cycloartane triterpenes from the fruit peel of Musa sapientum , 1998 .

[29]  M. Ali.  Neo-clerodane diterpenoids from Musa balbisiana seeds , 1992 .

[30]  J. Ibu,et al.  The Effect of Plantains (Musa paradisiaca) on DOCA-Induced Hypertension in Rats , 1991 .

[31]  A. Dyer,et al.  The INTERSALT study: relations of body mass index to blood pressure. INTERSALT Co-operative Research Group. , 1989, Journal of human hypertension.

[32]  S. Ghosal.  Steryl glycosides and acyl steryl glycosides from Musa paradisiaca , 1985 .

[33]  A. Das,et al.  A tetracyclic triterpenoid from Musa paradisiaca , 1983 .

[34]  H. Weissbach,et al.  Serotonin, Norepinephrine, and Related Compounds in Bananas. , 1958, Science.