Combined Electro-Optical Imaging for the Time Evolution of White Thrombus Growth in Artificial Capillaries

We present a novel methodology to measure white thrombus volume growth in an artificial microchannel, where whole blood flows. We designed a sensor consisting of a poly-dimethyl-siloxane microchannel and parallel gold electrodes sputtered on the surface of a slide, where induced hemostasis takes place. A novel inversion methodology, based on optical and electrical impedance data simultaneously processed, allows reconstructing the thrombus volume. The advantage of the proposed methodology is to reconstruct the evolution of the thrombus volume as a function of time; this is not possible with the present state of the art optical imaging based on confocal microscopy, which provides the thrombus volume estimation only at the end of the process.

[1]  Uwe Pliquett,et al.  Passive electrical properties of RBC suspensions: changes due to distribution of relaxation times in dependence on the cell volume fraction and medium conductivity , 1998 .

[2]  R. Specogna,et al.  Measurement bench for Impedance Tomography during hemostasis process in whole blood , 2011, 2011 IEEE International Symposium on Medical Measurements and Applications.

[3]  R. Specogna,et al.  Electrical Impedance Spectroscopy on flowing blood to predict white thrombus formation in artificial microchannels , 2012, 2012 IEEE International Instrumentation and Measurement Technology Conference Proceedings.

[4]  H. Fricke,et al.  A Mathematical Treatment of the Electric Conductivity and Capacity of Disperse Systems I. The Electric Conductivity of a Suspension of Homogeneous Spheroids , 1924 .

[5]  A. H. Ar-Rawi,et al.  Novel idea to monitor and measure blood hemoglobin noninvasively , 2010 .

[6]  H. Fricke,et al.  THE ELECTRIC RESISTANCE AND CAPACITY OF BLOOD FOR FREQUENCIES BETWEEN 800 AND 4½ MILLION CYCLES , 1925, The Journal of general physiology.

[7]  H. Schwan,et al.  Dielectric properties and ion mobility in erythrocytes. , 1966, Biophysical journal.

[8]  G. Thurston.  Plasma release-cell layering theory for blood flow. , 1989, Biorheology.

[9]  F G HIRSCH,et al.  The electrical conductivity of blood. I: Relationship to erythrocyte concentration. , 1950, Blood.

[10]  Ruben Specogna,et al.  A Discrete Geometric Approach to Cell Membrane and Electrode Contact Impedance Modeling , 2012, IEEE Transactions on Biomedical Engineering.

[11]  J. Ross Macdonald,et al.  Impedance spectroscopy , 2006, Annals of Biomedical Engineering.

[12]  P. Bettini,et al.  Electroquasistatic Analysis of the Gas Insulated Line for the ITER Neutral Beam Injector , 2009, IEEE Transactions on Magnetics.

[13]  K. Cole,et al.  Dispersion and Absorption in Dielectrics I. Alternating Current Characteristics , 1941 .

[14]  E. Barsoukov,et al.  Impedance spectroscopy : theory, experiment, and applications , 2005 .

[15]  Impedimetric monitoring of cell attachment on interdigitated microelectrodes , 2005 .

[16]  T. M. Nahir,et al.  Impedance Spectroscopy: Theory, Experiment, and Applications, 2nd ed Edited by Evgenij Barsoukov (Texas Instruments Inc.) and J. Ross Macdonald (University of North Carolina, Chapel Hill). John Wiley & Sons, Inc.: Hoboken, NJ. 2005. xvii + 596 pp. $125.00. ISBN 0471-64749-7. , 2005 .

[17]  H. Fricke,et al.  A Mathematical Treatment of the Electric Conductivity and Capacity of Disperse Systems ii. The Capacity of a Suspension of Conducting Spheroids Surrounded by a Non-Conducting Membrane for a Current of Low Frequency , 1925 .

[18]  Rob M. Heethaar,et al.  On the flow dependency of the electrical conductivity of blood , 2004, IEEE Transactions on Biomedical Engineering.